Vintage Molecular Biology
Summary: The module covers the historical evolution of molecular biological methods, which are the basis of today's modern methods of genome analysis. Contents of the module are molecular biological analyses of genes and genomes using BAC clones from high-density filters, creation of subclone libraries, Southern blot analyses with radio-labelled DNA probes, non-automated Sanger sequencing and polymerase chain reaction in water baths of different temperature using bacterially expressed Taq polymerase isolated by column chromatography.