Electron Microscopy Equipment

Our facility has all the equipment to guarantee excellent sample preparation and analysis.

These includes:

© A. Vogelsang

• Automated freeze substitution unit (Leica EM AFS2) for progressive lowering of temperature (PLT) embedding, freeze substitution and pipetting robot
• Reichert Knifemaker (Leica)
• Two ultramicrotomes
  • EM UC6 with IC90 E
  • Reichert Ultracut S
• High pressure freezer (Wohlwend HPF Compact 03)
• Motic Zoom- Stereomicroscope SMZ- 168
• Axiolab DRB kt Zeiss + Camera
• 80 kv Transmission Electron Microscope (Zeiss 906 E)

Services:

Sample preparation for electron microscopy

© A. Vogelsang

• Update into CFCI database- storage > 5 years
• Chemical fixation
• High pressure freezing (different carrier sizes)
  • CO2 Incubator (Teco 20) for culturing cells
• Freeze substitution (different cocktails)
• embedding of cells and tissue into epoxy (Epon araldite, LR White, Durcupan…)
  • flatt embedding
  • block embedding
• Embedding on Aclar
• Ultramicrotomy at room temperature
  • Ultrathin, semi thin, serial sections and Toluidin sectioning
• Poststaining and Goldstaining

Imaging:

© CFCI

• Data acquisition in transmission electron microscopy (TEM)
• Electron tomography (3D imaging)

Imaging Processing Software:

• Amira
• Arivis
• IMOT
• Fiji/ Image J

Facility:

• EM based fresh Chemicals and supplies for users
• Support and training in the methods listed in ,,Services”
• Support and training in special methods ( e.g Grid preparation..)
• Support and training in our electron microscope
• Access to all our equipment and EM Rooms
• Longterm storage into our Database
• Special access to our own Light microscopy Facility (if necessary)
  • Please look at Light microscopy Facility homepage