Confocal Laser Scanning Microscopy
In a confocal laser scanning microscope (CLSM), a focused laser scans the sample point by point. The emission light then passed through a spatially adjustable pinhole, whereby all the light which is not in the focal plane is cut off. Behind the emission filters located detectors (usually photo-multiplying tubes
In a confocal laser scanning microscope (CLSM), a focused laser scans the sample point by point. The emission light then passed through a spatially adjustable pinhole, whereby all the light which is not in the focal plane is cut off. Behind the emission filters located detectors (usually photo-multiplying tubes - PMT's) collect and amplify the light coming from each point. That's why there is NO CAMERA on a confocal microscope!
More information about the principles and the history of confocal microscope you can find here
