Zeiss Observer with ApoTome (ApoTome II)

Description

Zeiss Apotome II at CFCI © A. Vogelsang

Location: MTZ, 2nd floor; room B 20.001B

This microscope is designed for routine work on fixed or living samples. Optical sectioning can be obtained on fixed samples by using the ApoTome (structured illumination). It'suitable for live cell imaging on fluorescent labeled cells because of 7 fast switchable Colibri LEDs. The microscope is also capable for brightfield including contrast techniques like phase contrast, darkfield and polarized light.

Suitable for

Mouse intestine section: Alexa 350 (blue), SYTOX green, Alexa 568 (orange)]

  • Living and fixed samples
  • Histological colored samples
  • Fluorescence colored samples
  • Structured illumination with ApoTome, tile or mosaic scans, z-stacks, multi color imaging, timelapse
  • Brightfield Imaging with DIC or phase contrast


     
Microscope Stand
Stand orientation Inverted                
Condenser LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC II, DIC III,
motorized
Microscope Details
Objectives     

Zeiss A-Plan 5x / 0.12
Zeiss Plan-Apochromoat 10x / 0.45. Ph 1
Zeiss LD Plan-Neofluar 20x/0.4 Korr Ph2
Zeiss Plan-Apochromat 20x/0,8
Zeiss Plan-Apochromat 40x/0,95 Korr
Zeiss Plan-Apochromat 63x / 1.4 Oil
XY stage Motorized, inserts for dishes, slides and multiwell-plates
Z focus device Manual, motorized
Illumination Transmitted light (microLED) Fluorescence (Colibri 7) Colibri LED's:
385nm, 430nm, 475nm, 511nm 555nm, 590nm, 630nm
Fluorescent filter cube

Quad 90 HE LED
DAPI / GFP / RFP / Cy3
[BS 405, EM 425/30  412-440nm]
[BS 493, EM 514/30  499-529nm]
[BS 575, EM 592/30  579-604nm]
[BS 653, EM 709/100 659-759nm]

Triple 92 HE LED
DAPI / GFP / mCherry
[BS 405, EM 425/30   412-433nm]
[BS 493, EM 524/50   501-547nm]
[BS 610, EM 688/145 617-758nm]
Detection                             

Monochrome Kamera AxioCam 506 mono
6 MegaPixel, 14 Bit,
Pixelgröße 4,54 µm x 4,54 µm
Chipgröße: 12 mm x 10 mm, entspricht 1" (Diagonale 16 mm)

Color camera Axiocam 506 color     
6 MegaPixel, 14 Bit,
Pixel size: 4,54μm x 4,54μm
Image sensor size: 12mm x 10mm, is equal to 1" (Diagonale 16mm)
Computer
RAM 16 GB                                                                    
Grafic card AMD FirePro V4900
Software Zen 2.3
Display TFT 27" HP Z27n
More information
Incubation Temperature and CO2                                         
Additional Equpiment          ApoTome II (Optical sectioning using structured illumination),
Definite Focus (Function against z-Drift),

Experiment Designer,
Extended Depth of Focus
Inventory list  
Date of installation 03.07.2018
Manufacturer Zeiss

About this page

Silke Tulok
Last modified: Aug 17, 2022