Leica SP8 - Mouse intravital microscopy
Multiphoton laser scanning microscopy
A multiphoton laser scanning microscope is more or less a confocal microscope equipped with a special infrared laser allowing for multi-photon excitation. This laser enables 2-photon or 3-photon excitation and both second~ and third harmonic generation providing deep tissue/organ imaging in small, living animals.
Description
Location: MTZ, 1st floor; room B 10.019
The Leica SP8 MP/OPO is designed and optimized for intravital microscopy of small animals (mostly mice, but rat and zebrafish would also be possible). The tunable IR-laser (Chameleon Vision II Compact OPO) permits fluorophore multiphoton excitations in the NIR range from 680nm up to 1280nm providing deep tissue/organ imaging in living animals. Our microscope room is furthermore equipped a surgery area and a complete anesthesia unit to enable onsite surgery together with imaging.
Suitable for
- Mouse intravital Microscopy
| Microscope Stand | |
|---|---|
| Stand orientation: | upright, Leica DM 6000 CFS flex, fixed stage |
| Condenser: | demounted |
| Additional Magnification: | no |
| Microscope Details | |
|---|---|
| Scan head |
Non resonant mode: |
| Objectives |
Leica HC PL FLUOTAR 5x / 0.15 |
| XY stage | motorized |
| Z focus device | manual/ motorized |
| Illumination |
reflected light (LED) |
| Fluorescent filter cubes |
SHG/CFP/far red (BS 495: BP 450/70 & BP710/40) |
| Emission filter wheel |
filter system I3 (BP 450-490, LP 515), for e.g. FITC, GFP |
| NDD-Detection | 2x HyD SP GaAsPDetektor 2x Alkali-PMT (Hamamatsu R 9624) |
| Internal detection |
3 channel spectral detection via Pellin Broca prisma |
| Resolution | max. 8192 x 8192 |
| Computer | |
|---|---|
| CPU: | Intel 8Core Xeon E52650 2 GHz |
| RAM: | 16GB |
| Grafic card: | NVIDIA Quadro K600 1GB |
| Software: | LAS X |
| Display: | 30" High brilliance LCD, 2560 x 1600 Pixel |
| More information | |
|---|---|
| Laser safety | Microscope stage is sealed in a laser safety box |
| Additional Equpiment | anesthesia unit, small animal ventilation system & heating plate both at the microscope stage and surgery area, heating lamp |
| Inventory list | 0095080/00 |
| Date of installation | August 2014 |
| Manufacturer | Leica |
© Stephan Wiegand
Intravital Specialist
NameDr. Michael Gerlach
Training, scientific support and advice
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Visiting address:
Medizinisch Theoretisches Zentrum, Office: A 20.044 Fiedlerstraße 42
01307 Dresden