Leica SP8 - Mouse intravital microscopy

Multiphoton laser scanning microscopy

A multiphoton laser scanning microscope is more or less a confocal microscope equipped with a special infrared laser allowing for multi-photon excitation. This laser enables 2-photon or 3-photon excitation and both second~ and third harmonic generation providing deep tissue/organ imaging in small, living animals.

Description

The Leica SP8 MP/OPO is designed and optimized for intravital microscopy of small animals (mostly mice, but rat and zebrafish would also be possible). The tunable IR-laser (Chameleon Vision II Compact OPO) permits fluorophore multiphoton excitations in the NIR range from 680nm up to 1280nm providing deep tissue/organ imaging in living animals. Our microscope room is furthermore equipped a surgery area and a complete anesthesia unit to enable onsite surgery together with imaging.

Suitable for

• Mouse intravital Microscopy

Microscope Stand
Stand orientation:	upright, Leica DM 6000 CFS flex, fixed stage
Condenser:	demounted
Additional Magnification:	no

Microscope Details
Scan head	Non resonant mode: up to 1800 Hz, 7fps (512 x 512) Resonant mode: up to 12000 Hz, 40fps (512 x 512)
Objectives	Leica HC PL FLUOTAR 5x / 0.15 Leica HCXPOL 10x / 0.4 W (Wd = 3.6 mm) Leica HCX IRAPO L25x / 0.95 W (Wd = 2.5 mm) Leica HC PL IRAPO 40x / 1.10 W (Wd = 0.65 mm)
XY stage	motorized
Z focus device	manual/ motorized
Illumination	reflected light (LED) Fluorescence (HXP) Leica EL 6000 Laser Argon 458nm, 488nm, 514nm Laser HeNe 561nm Laser DPSS 633nm Ti:Sa: MP 680nm - 1040nm OPO 680nm - 1280nm
Fluorescent filter cubes	SHG/CFP/far red (BS 495: BP 450/70 & BP710/40) GFP/RFP (BS 560: BP525/50 & BP617/73) mCherry/ Cy5 (BS 650:  BP 624/40 & 650/50)
Emission filter wheel	filter system I3 (BP 450-490, LP 515), for e.g. FITC, GFP filter system N2.1 (BP 515-560, LP 590), for e.g. TRITC, Cy3
NDD-Detection	2x HyD SP GaAsPDetektor 2x Alkali-PMT (Hamamatsu R 9624)
Internal detection	3 channel spectral detection via Pellin Broca prisma 1x HyD SP GaAsPDetektor 2x Alkali-PMT (Hamamatsu R 9624 ) Detectionrange 400-800nm
Resolution	max. 8192 x 8192

Computer
CPU:	Intel 8Core Xeon E52650 2 GHz
RAM:	16GB
Grafic card:	NVIDIA Quadro K600 1GB
Software:	LAS X
Display:	30"  High brilliance  LCD, 2560 x 1600 Pixel

More information
Laser safety	Microscope stage is sealed  in a laser safety box
Additional Equpiment	anesthesia unit, small animal ventilation system & heating plate both at the microscope stage and surgery area, heating lamp
Inventory list	0095080/00
Date of installation	August 2014
Manufacturer	Leica