Development of molecular markers for the assignment and differentiation of breeding material
Since mobile DNA elements and repetitive, structure-determining genome regions change rapidly in evolutionary time intervals, they are ideally suited as molecular markers. Our robust marker systems have already been tested for genotype differentiation in potatoes, camellias, beets, poplars and spruces and are used in the breeding routines of several companies.
Left: Phenotypic variation in potato. Right: Retrotransposon-based markers for the differentiation of potato cultivars.
Selected publications
Reiche B., Kögler A., Morgenstern K., Brückner M., Weber B., Heitkam T., Seibt K. M., Tröber U., Meyer M., Wolf H., Schmidt T., Krabel D. (2020): Anwendung des SINE-basierten Markersystems ISAP zur Identifizierung von Pappelklonen. In: Liesebach M (ed) Thünen Report 76: Forstpflanzenzüchtung für die Praxis. Johann Heinrich von Thünen-Institut, Braunschweig: 144-154 read article
Wenke T., Seibt K. M., Döbel T., Muders K. and Schmidt T. (2015): Inter-SINE Amplified Polymorphism (ISAP) for rapid and robust plant genotyping. In: Volume of Plant Genotyping of the series "Methods in Molecular Biology"; Humana Press, USA (Springer publishing group), 1245: 183-192 read article
Seibt K., Wenke T., Wollrab C., Junghans H., Muders K., Dehmer K., Diekmann K., Schmidt T. (2012): Development and application of SINE-based markers for genotyping of potato varieties. Theor Appl Genet 125(1):185-196 read article