Effect of high temperature on gene expression, phytochemical profile and in vitro bioactivity of seedlings: Case study of broccoli (Brassica oleracea botrytis var. cymosa)
Plants challenged with high temperature undergo many adaptive mechanisms at molecular levels to keep normal physiology function. They can adapt to different temperatures by changing their transcriptome, proteome and metabolome, or even by activating cell death mechanisms. Such adaptability is due to their intensive shifts in biochemical pathways, which, as a consequence, can significantly change their bioactivity as well. In this project our goal is to identify genes and metabolites of brocolli seedlings that have been altered by high temperature, to quantify the intensity of their changes, and to define possible correlations between those parameters and seedlings bioactivity. The chain interactions between temperature shift and plant genes expression and biochemical properties will be assessed employing qRT-PCR analysis, targeted specific metabolomics approach, and statistical data analyses in order to show a tentative pattern of environment temperature effect on plant physiology.
Collaboration with Dr. Ivana Sola (University of Zagreb)
funded by: Deutscher Akademischer Austauschdienst
