12.06.2025
Unser Artikel "Protocol for long-term ex vivo cultivation and imaging of Drosophila imaginal discs" wurde in STAR Protocols veröffentlicht
Protocol for long-term ex vivo cultivation and imaging of Drosophila imaginal discs
Drosophila wing and eye-antennal imaginal discs are excellent models for studying tissue development and cell behavior. Here, we present a protocol for long-term cultivation and imaging of wing and eye-antennal imaginal discs. We describe the mounting, dissection, and ex vivo cultivation of imaginal discs. We further detail 4D time-lapse image acquisition with minimized phototoxicity and image restoration using a machine-learning-based algorithm. This protocol is suitable for studying dynamic cell behaviors with single-cell resolution during normal and perturbed development.
Sui, L.; Dahmann, C.