Publications
2022
On the role of cell surface associated, mucin-like glycoproteins in the pennate diatom Craspedostauros australis (Bacillariophyceae)
Poulsen, N.*, Hennig, H., Geyer, V. F., Diez, S., Wetherbee, R., Fitz-Gibbon, S., Pellegrini, M., Kröger, N.*
Journal of Phycology (2022) accepted
Shedding light on silica biomineralization by comparative analysis of the silica-associated proteomes from three diatom species
Skeffington, A*., Gentzel, M., Ohara, A., Milentyev, A., Heintze, C. Böttcher, L., Görlich, S. Shevchenko, A., Poulsen, N., Kröger, N*.
The Plant Journal (2022) 110, 1700-1716
Structure and morphogenesis of the frustule
Babenko, I., Friedrich, B.M., Kröger, N.
The Molecular Life of Diatoms, (eds Falciatore, A., Mock, T.) Springer (2022) p. 287-312
Biomolecules involved in frustule biogenesis and function
Kröger, N.
The Molecular Life of Diatoms, (eds Falciatore, A., Mock, T.) Springer (2022) p. 313-343
Diatom adhesion and motility
Poulsen, N., Davutoglu, M.G., Zackova Suchanova, J.
The Molecular Life of Diatoms, (eds Falciatore, A., Mock, T.) Springer (2022) p. 367-393
2021
Diurnal transcript profiling of the diatom Seminavis robusta reveals adaptations to a benthic lifestyle
Bilcke, G., Osuna-Cruz, C.M., Santana Silva, M., Poulsen, N., D’hondt, S., Bulankova, P., Vyverman, W., De Veylder, L. and Vandepoele, K.
The Plant Journal (2021)107,315–336
Computational Analysis of the Effects of Nitrogen Source and Sin1 Knockout on Biosilica Morphology in the Model Diatom Thalassiosira pseudonana
S. Horvát, A. Fathima, S. Görlich, M. Schlierf*,C. D. Modes*, N. Kröger*
Discover Materials 1, Article number: 8, 2021
Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta
G. Bilcke, K. Van den Berge, S. De Decker, E. Bonneure, N. Poulsen, P. Bulankova, C. M. Osuna-Cruz, J. Dickenson, K. Sabbe, G. Pohnert, K. Vandepoele, S. Mangelinckx, L. Clement, L. De Veylder, W. Vyverman
The ISME Journal 15, 562–576, 2021
2020
Natural hybrid silica/protein superstructure at atomic resolution
S. Görlich, A. J. Samuel, R.J. Best, R. Seidel, J. Vacelet, F. K.Leonarski, T. Tomizaki, B. Rellinghaus, D. Pohl, I. Zlotnikov*
PNAS, 202019140, 2020
An intimate view into the silica deposition vesicles of diatoms
C. Heintze, P. Formanek, D. Pohl, J. Hauptstein, B. Rellinghaus, N. Kröger
BMC Materials 2, Article number: 11, 2020
Chitin synthase localization in the diatom Thalassiosira pseudonana
M. Wustman, N. Poulsen, N. Kröger, K. H. van Pée
BMC Materials 2, Article number: 10, 2020.
The Seminavis robusta genome provides insights into the evolutionary adaptations of benthic diatoms
C. Osuna-Cruz et al.
Nature Communications, 11, Article: 3320, 2020.
Genetically Programmed Regioselective Immobilization of Enzymes in Biosilica Microparticles
E. Kumari, S. Görlich, N. Poulsen, N. Kröger
Adv. Funct. Mater., 30, 2000442, 2020.
2019
Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis
M. Lachnit, M.T. Buhmann, J. Klemm, N. Kröger, N. Poulsen*
Phil. Trans. R. Soc. B., 374: 20190196, 2019.
Control of biosilica morphology and mechanical performance by the conserved diatom gene Silicanin-1
S. Görlich, D. Pawolski, I. Zlotnikov, N. Kröger
Commun. Biol. 2, 245, 2019.
Influence of silica architecture on the catalytic activity of immobilized glucose oxidase
G. Begum, C. Oschatz, M. Oschatz, S. Kaskel, E. Brunner, N. Kröger
Bioinspired Biomimetic and Nanobiomaterials 8, 72-80, 2019.
2018
Biomineralization as a paradigm of directional solidification: A physical model for molluscan shell ultrastructural morphogenesis
V. Schoeppler, L. Gránásy, E. Reich, N. Poulsen, R. de Kloe, P. Cook, A. Rack, T. Pusztai, I. Zlotnikov*
Adv. Mater. 30 , 1803855, 2018.
Reconstituting the formation of hierarchically porous silica patterns using diatom biomolecules
D. Pawolski, C. Heintze, I. Mey, C. Steinem, N. Kröger
J. Struct. Biol. 204, 64-74, 2018.
2017
Immobilization of Proteins on Diatom Biosilica
N. Kröger, N. C. Dubey, E. Kumari
In: Diatom Nanotechnology (D. Losic, ed.), RSC Publishing p. 126-149, 2017.
Silicanin-1 is a conserved diatom membrane protein involved in silica biomineralization
A. Kotzsch, P. Gröger, D. Pawolski, P. H. H. Bomans, N. A. J. M. Sommerdijk, M. Schlierf, N. Kröger
BMC Biol. 15, 65, 2017.
2016
Establishing super-resolution imaging for proteins in diatom biosilica
P. Gröger, N. Poulsen, J. Klemm, N. Kröger, M. Schlierf
Scientific Reports 6, Article number: 36824, 2016.
Structures of the PSCD domains of pleuralin-1, a biosilica-associated protein from the diatom Cylindrotheca fusiformis, and their interactions with other cell wall proteins
S. De Sanctis, M. Wenzler, N. Kröger, W. M. Malloni, M. Sumper, R. Deutzmann, P. Zadravec, E. Brunner, W. Kremer, H. R. Kalbitzer
Structure 24, 1178–1191, 2016.
Biochemical composition and assembly of biosilica-associated insoluble organic matrices from the diatom Thalassiosira pseudonana
A. Kotzsch, D. Pawolski, A. Milentyev, Anna Shevchenko, A. Scheffel, N. Poulsen, Andrej Shevchenko, N. Kröger
J. Biol. Chem. 291, 4982-4997, 2016.
2015
Targeted drug delivery using genetically engineered diatom biosilica
B. Delalat, V. Sheppard, S. Rasi Ghaemi, S. Rao, C. Prestidge, G. McPhee, M.-L. Rogers, J. Donoghue, V. Pillay, T. Johns, N. Kröger*, N. Voelcker*
Nat. Commun. 6, 8791, 2015.
Compartmentalisation of Enzymes for Cascade Reactions through Biomimetic Layer-by-Layer Mineralization
G. Begum, W. B. Goodwin, B. M. deGlee, K. H. Sandhage, N. Kröger
J. Mater. Chem. B, 3, 5232-5240, 2015.
2014
A Tyrosine-rich Cell Surface Protein in the Diatom Amphora coffeaeformis identified through Transcriptome Analysis and Genetic Transformation
M. T. Buhmann, N. Poulsen, J. Klemm, M. R. Kennedy, C. D. Sherrill, N. Kröger
PLOS ONE 9, e110369, 2014.
Complex-shaped microbial biominerals for nanotechnology
N. Kröger, E. Brunner
WIREs Nanomed. Nanobiotechnol. 6, 615-627, 2014.
Isolation and biochemical characterization of underwater adhesives from diatoms
N. Poulsen*, N. Kröger , M. J. Harrington, E. Brunner, S. Paasch and M. T. Buhmann
Biofouling 30, 513-523, 2014.
2013
Nitrate and carbonate concentration for high glucose content in microalgae
N. Kröger, S. J. Miller, N. Poulsen
US patent US8569040 B1 (approved).
Pentalysine clusters mediate silica targeting of silaffins in Thalassiosira pseudonana
N. Poulsen, A. Scheffel, V. C. Sheppard, P. M. Chesley, N. Kröger
J. Biol Chem. 288, 20100-20109, 2013.
Kieselalgen für die Nanotechnik
N. Kröger
Nachrichten aus der Chemie 61, 514-518, 2013.
Rapid Flow-through Biocatalysis with High Surface Area, Enzyme -loaded Carbon and Gold- bearing Diatom Frustule Replicas
S. C. Davis, V. C. Sheppard, G. Begum, Y. Cai, Y. Fang, J. D. Berrigan, N. Kröger,* Kenneth H. Sandhage*
Adv. Funct. Mater. 23, 4611-4620, 2013.
Morphogenese der Silica-Zellwände von Diatomeen
N. Kröger
BIOspektrum 19, 12-15, 2013.
2012
Live Diatom Silica Immobilization of Multimeric and Redox-active Enzymes
V. C. Sheppard, A. Scheffel, N. Poulsen, N. Kröger
Appl. Environ. Microbiol. 78, 211-218, 2012.
2011
Biocatalytic Nanoscale Coatings through Biomimetic Layer-by-Layer Mineralization
N. R. Haase, S. Shian, K. H. Sandhage, N. Kröger
Adv. Funct. Mater. 21, 4243-4251, 2011.
Nanopatterned protein microrings from a diatom that direct silica morphogenesis
A. Scheffel, N. Poulsen, S. Shian, N. Kröger
Proc. Natl. Acad. Sci. USA 108, 3175-3180, 2011.
2010
From Diatom Biomolecules to Bioinspired Syntheses of Silica- and Titania-based Materials.
N. Kröger, K. H. Sandhage
MRS Bull. 35, 122-126, 2010.
Characterization of an endoplasmic reticulum-associated silaffin kinase from the diatom Thalassiosira pseudonana
V. C. Sheppard, N. Poulsen, N. Kröger
J. Biol. Chem. 285, 1166-1176, 2010.
2009
Protein-Mediated Layer-by-Layer Syntheses of Freestanding Microscale Titania Structures with Biologically Assembled 3-D Morphologies
Y. Fang, Q. Wu, M. B. Dickerson, Y. Cai, S. Shian, J. Berrigan, N. Poulsen, N. Kröger, K. H. Sandhage
Chem. Mater. 21, 5704-5710, 2009.
The molecular basis of nacre formation
N. Kröger
Science 325, 1351-1352, 2009.
Protein-enabled synthesis of monodisperse titania nanoparticles on and within polyelectrolyte matrices
E. Kharlampieva, J. M. Slocik, S. Singamaneni, N. Poulsen, N. Kröger, R. R. Naik, V. V. Tsukruk
Adv. Funct. Mat. 19: 2303-2311, 2009.
2008
Diatoms - from cell wall biogenesis to nanotechnology
N. Kröger, N. Poulsen
Annu. Rev. Genet. 42, 83-107, 2008.
The Phaeodactylum genome reveals the evolutionary history of diatom genomes
C. Bowler, et al.
Nature. 456, 239-244, 2008.
Bio-enabled Surface-mediated Growth of Uniform Titania Nanoparticles
E. Kharlampieva, T. Tsukruk, J. M. Slocik, H. Ko, N. Poulsen, R. R. Naik, N. Kröger, V. V. Tsukruk
Adv. Mater. 20, 3274-3279 (2008).
Identification of peptides capable of inducing the formation of titania but not silica via a subtractive bacteriophage display approach
Y. Fang, N. Poulsen, M. B. Dickerson, Y. Cai, S. E. Jones, R. R. Naik, N. Kröger, K. H. Sandhage
J. Mater. Chem. 18, 3871-3875, 2008.
2007
Prescribing diatom morphology: toward genetic engineering of biological nanomaterials
N. Kröger
Cur. Opin. Chem. Biol. 11, 662-669, 2007.
Biochemistry and Molecular Genetics of Silica Biomineralization in Diatoms
N. Kröger, N. Poulsen
Handbook of Biomineralization - Biological Aspects and Structure Formation (E. Baeuerlein, ed.), Wiley-VCH, p. 43-58, 2007.
Silica immobilization of an enzyme via genetic engineering of the diatom Thalassiosira pseudonana
N. Poulsen, C. Berne, J. Spain, N. Kröger
Angew. Chem. Int. Ed. 46, 1843-1846, 2007.
Identification and Design of Peptides for the Rapid, High Yield Formation of Nanoparticulate TiO2 from Aqueous Solutions at Room Temperature
M. B. Dickerson, S. E. Jones, Y. Cai, G. Ahmad, R. R. Naik, N. Kröger, K. H. Sandhage
Chem. Mater. 20, 1578-1584, 2007.
2006
Bio-enabled synthesis of Rutile (TiO2) at ambient temperature and neutral pH
N. Kröger, M. B. Dickerson, G. Ahmad, Y. Cai, M. S. Haluska, K. H. Sandhage, N. Poulsen, V. C. Sheppard
Angew. Chem. Int. Ed. 45, 7239-7243, 2006.
Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae)
N. Poulsen, P. M. Chesley, N. Kröger
J. Phycol. 42, 1059-1065, 2006.
Rapid, Room-Temperature Formation of Crystalline Calcium Molybdate Phosphor Microparticles via Peptide-Induced Precipitation
G. Ahmad, M. B. Dickerson, C. Church, Y. Cai, S. E. Jones, R. R. Naik, J. S. King, J. C. Summers, N. Kröger, K. H. Sandhage
Adv. Mater. 18, 1759-1763, 2006.
2005
Biosilica Nanofabrication in Diatoms: The Structures and Properties of Regulatory Silaffins
N. Kröger, N. Poulsen
MRS Proceedings, 873, K1.2 doi:10.1557/PROC-873-K1.2, 2005.
A new molecular tool for transgenic diatoms: Control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette
N. Poulsen, N. Kröger
FEBS J. 272, 3413-3423, 2005.
2004
Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana
N. Poulsen, N. Kröger
J. Biol. Chem. 279, 42993-42999, 2004.
The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism
E. V. Ambrust, et al.
Science 306, 79-86, 2004.
The Molecular Basis of Diatom Biosilica Formation
N. Kröger, M. Sumper
in Biomineralization (E. Baeuerlein, ed.) 2nd edition, Wiley-VCH, p. 137-158, 2004.
Silica formation in diatoms: the function of long-chain polyamines and silaffins
M. Sumper, N. Kröger
J. Mater. Chem. 14, 2059-2065, 2004.
2003
Biosilica formation in diatoms: Characterization of native silaffin-2 and its role in silica morphogenesis
N. Poulsen, M. Sumper, N. Kröger
Proc. Natl. Acad. Sci. USA 100, 12075-12080, 2003.
Solid-state 29Si MAS NMR studies of diatoms: structural characterization of biosilica deposits
R. Bertermann, N. Kröger, R. Tacke
Anal. Bioanal. Chem. 375, 630-634,2003.
Silica deposition by a repetitive proline-rich protein from systemically resistant cucumber plants
H. Kauss, K. Seehaus, R. Franke, S. Gilbert, R. Dietrich, N. Kröger
Plant J. 33, 87-95, 2003.
2002
Self-assembly of highly phosphorylated silaffins and their function in biosilica morphogenesis
N. Kröger, S. Lorenz, E. Brunner, M. Sumper
Science 298, 584-586, 2002.
2001
The sweetness of diatom molecular engineering
N. Kröger
J. Phycol. 37, 657-658, 2001.
Silica precipitating peptides from diatoms: The chemical structure of silaffin-1A from Cylindrotheca fusiformis
N. Kröger, R. Deutzmann, M. Sumper
J. Biol. Chem. 276, 26066-26070, 2001.
1H, 13C and 15N sequence-specific resonance assignment of the PSCD4 domain of diatom cell wall protein pleuralin-1
M. Wenzler, E. Brunner, N. Kröger, G. Lehmann, M. Sumper, H. R. Kalbitzer
J. Biomolec. NMR 20, 191-192, 2001.
The biogenesis of silicate cell walls of diatoms
N. Kröger
BIOspektrum 6, 556-557, 2001.
2000
Species specific polyamines from diatoms control silica morphology
N. Kröger, R. Deutzmann, C. Bergsdorf, M. Sumper
Proc. Natl. Acad. Sci. USA 97, 14133-14138, 2000.
Pleuralins are involved in theca differentiation in the diatom Cylindrotheca fusiformis
N. Kröger, R. Wetherbee
Protist 151, 263-273, 2000.
The Biochemistry of Silica Formation in Diatoms
N. Kröger, M. Sumper
in Biomineralization (E. Baeuerlein, ed.) 1st edition, Wiley-VCH, p. 151-170, 2000.
1999
Polycationic peptides from diatom biosilica that direct silica nanosphere formation
N. Kröger, R. Deutzmann, M. Sumper
Science 286, 1129-1132, 1999.
Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae)
H. Fischer, I. Robl, M. Sumper, N. Kröger
J. Phycol. 35, 113-120, 1999.
1998
Diatom cell wall proteins and the cell biology of silica biomineralization
N. Kröger and M. Sumper
Protist 149, 213-219,1998.
1997
Characterization of a 200 kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall
N. Kröger, G. Lehmann, R. Rachel, M. Sumper
Eur. J. Biochem. 250, 99-105, 1997.
1996
Frustulins: domain conservation in a protein family associated with diatom cell walls
N. Kröger, C. Bergsdorf, M. Sumper
Eur. J. Biochem. 239, 259-264, 1996.
1994
A new calcium binding glycoprotein family constitutes a major diatom cell wall component
N. Kröger, C. Bergsdorf, M. Sumper
The EMBO Journal (1994)13:4676-4683