Equipment
The following devices and work stations are available.
...extracellular recording action potentials and local field potentials from
- 2-D cultured cells
- organoids
- fresh tissue slices
- in vivo aquatic larvae
2-D neuron culture in one quadrant of an 8x8 recording grid (left). Round organiod on an 8x8 recording grid held in place with a net (middle). Acute coronal brain slice in MEA1060-Inv-BC recording well with superfusion and drainage tubes (right).
Device: MEA1060-Inv-BC (multichannel systems) operated via MC_Rack
MEA1060-Inv-BC set up in use for acute brain slice recording with current stimulation.
Mounted on an inverted microskope (Nikon Eclipse Ti-E/B) with image taking via an interline CCD camera (Andor Clara).
Nikon Eclipse Ti-E/B and software
Available Objectives:
- 2x Plan Apo λ (NA 0.1, WD 8.5)
- 4x Plan Fluor (NA 0.13, WD 17.2)
- 10x Plan Fluor (NA 0.3, WD 16)
- 20x S Plan Fluor ELWD (NA 0.45, WD 8.2-6.9)
- Additional 1.5x magnification
Available filters:
- CFP
- GFP
- TxRed
- Cy5
Additional filter needs can be discussed.
Combinable with:
- Heat control (internal heating plate (cultures) and inline heater (acute tissue))
- Superfusion and drainage of fresh solutions
- Bathapplication of modulators
- Electric stimulation (technical specifications)
- Optic stimulation (wavelengths (in nm) 470, 513, 580, 640; combinable with the aforementioned filters; intensity scaleable; temporally patterned stimulation possible)
- Mechanic stimulation
...extracellularly recording action potentials and local field potentials from
Maestro Edge (Axion) recording station with Lumos24
- 2-D cultured cells
- organoids
- organotypic slices
- fresh tissue slices
One well in a 6-well plate with 64 electrodes and a neuronal culture (imaged with Nikon Eclipse Ti-E)
Combinable with
- CO2 control
- Heat control
- Bath application of modulators
- Electric stimulation
- Optic stimulation
- Sample imaging
Device: Maestro Edge (Axion) with Lumos24 operated via AxIS Software.
...in a CMOS-MEA (multichannel systems) for recording from cell cultures and acute slices.
CMOS-MEA500 (multichannel systems)
1024 integrated stimulation sites.
Combinable with:
- Heat control (internal heating plate (cultures) and inline heater (acute tissue))
- Superfusion and drainage of fresh solutions
- Bathapplication of modulators
In the process of establishment.
A work station with e-phys specific software is available for data handling.
Data handling work station
HeraCell 150i with standard settings
Currently, we provide a total of six shelves in two incubators (HeraCell 150i).
Standard settings: 37°C, 5% CO2.
Diverging settings can be arranged.
Optional with orbital shaking plate.
In addition, we can provide incubation in a compact table top CO2 incubator (Midi40).
Table top incubator
For microbiological work including all standard plastic ware and instruments.
HeraSafe KS laminar flow safety cabinet
For generating fresh tissue slices a sequentially ordered set of equipment, including consumables, is available:
Anaesthesia area
Induction boxes (ambient air)
Peristaltic pump for transcardiac perfusion
Dissection place for organ removal and blocking
Vibration microtome for tissue slicing
Warm water bath for slice incubation
Supply of carbogen at all sections.
Acute slice preparation work flow station
For generating artificial cerebrospinal fluids and other soultions a fine scale, osmometer and pH meter (Seven Compact by Mettler Toledo) with a semi-micro electrode is available.
Solution mixing and testing work space
The work station for surface treatments of the recording arrays (cleaning, hydrophilization) includes, amongst others, a plasma cleaner and UV curer. The plasma cleaner is in the process of establishment and will be operated with pure oxygen.
Array maintenance work station
Electrotechnical and fine mechanical work station
A work station with soldering iron, UV curing light and further fine mechanical and electrotechnical equipment is available.
The following recording devices are in the process of establishment:
- CMOS MEA (multichannel systems)
- Patch clamp