Publications
2024
Generation and application of novel hES cell reporter lines for the differentiation and maturation of hPS cell‑derived islet‑like clusters. Elisa Zanfrini, Manuj Bandral, Luka Jarc, Maria Alejandra Ramirez‑Torres, Daniela Pezzolla, Vida Kufrin, Eva Rodriguez‑Aznar, Ana Karen Mojica Avila, Christian Cohrs, Stephan Speier, Katrin Neumann & Anthony Gavalas. Sci Rep 14, 19863 (2024). https://doi.org/10.1038/s41598-024-69645-4
The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex. Paula Cubillos, Nora Ditzer, Annika Kolodziejczyk, Gustav Schwenk, Janine Hoffmann, Theresa M Schütze, Razvan P Derihaci, Cahit Birdir, Johannes EM Köllner, Andreas Petzold, Mihail Sarov, Ulrich Martin, Katherine R Long, Pauline Wimberger & Mareike Albert.
The EMBO Journal. 2024 Mar. DOI: 10.1038/s44318-024-00068-7. PMID: 38514807.
Activation of recombinases at specific DNA loci by zinc-finger domain insertions.
Liliya Mukhametzyanova, Lukas Theo Schmitt, Julia Torres-Rivera, Teresa Rojo-Romanos, Felix Lansing, Maciej Paszkowski-Rogacz, Heike Hollak, Melanie Brux, Martina Augsburg, Paul Martin Schneider & Frank Buchholz.
Nat Biotechnol (2024). https://doi.org/10.1038/s41587-023-02121-y
Regulation of multiple signaling pathways promotes the consistent expansion of human pancreatic progenitors in defined conditions.
Luka Jarc, Manuj Bandral, Elisa Zanfrini, Mathias Lesche, Vida Kufrin, Raquel Sendra, Daniela Pezzolla, Ioannis Giannios, Shahryar Khattak, Katrin Neumann, Barbara Ludwig & Anthony Gavalas.
eLife, 12, RP89962. https://doi.org/10.7554/eLife.89962
2023
Hemophagocytic lymphohistiocytosis-like hyperinflammation due to a de novo mutation in DPP9.
Christine Wolf, Hannah Fischer, Lörn-Sven Kühl, Sarah Koss, Rami Abou Jamra, Sven Starke, Jurek Schultz, Stephan Ehl, Katrin Neumann, Catharina Schuetz, Robert Huber, Veit Hornung & Min Ae Lee-Kirsch.
The Journal of allergy and clinical immunology, S0091-6749(23)00978-8. Advance online publication. https://doi.org/10.1016/j.jaci.2023.07.013
Reliability of human retina organoid generation from hiPSC-derived neuroepithelial cysts.
Madalena Carido, Manuela Völkner, Lisa Maria Steinheuer, Felix Wagner, Thomas Kurth, Natalie Dumler, Selen Ulusoy, Stephanie Wieneke, Anabel Villanueva Norniella, Cristina Golfieri, Shahryar Khattak, Bruno Schönfelder, Maria Scamozzi, Katja Zoschke, Sebastian Canzler, Jörg Hackermüller, Marius Ader & Mike O. Karl
Frontiers in cellular neuroscience, 17, 1166641. https://doi.org/10.3389/fncel.2023.1166641
Dynamic matrices with DNA-encoded viscoelasticity for cell and organoid culture.
Yu-Hsuan Peng, Syuan-Ku Hsiao, Krishna Gupta, André Ruland, Günter K. Auernhammer, Manfred F. Maitz, Susanne Boye, Johanna Lattner, Claudia Gerri, Alf Honigmann, Carsten Werner & Elisha Krieg.
Nature nanotechnology, 18(12), 1463–1473. https://doi.org/10.1038/s41565-023-01483-3
2022
HBEGF-TNF induce a complex outer retinal pathology with photoreceptor cell extrusion in human organoids
Manuela Völkner, Felix Wagner, Lisa Maria Steinheuer, Madalena Carido, Thomas Kurth, Ali Yazbeck, Jana Schor, Stephanie Wieneke, Lynn J. A. Ebner, Claudia Del Toro Runzer, David Taborsky, Katja Zoschke, Marlen Vogt, Sebastian Canzler, Andreas Hermann, Shahryar Khattak, Jörg Hackermüller & Mike O. Karl
Nature Communications 2022 volume 13
FoxP3 expression by retinal pigment epithelial cells: transcription factor with potential relevance for the pathology of age-related macular degeneration
Ahmad Samir Alfaar, Lucas Stürzbecher, Maria Diedrichs-Möhring, Marion Lam, Christophe Roubeix, Julia Ritter, Kathrin Schumann, Balasubramaniam Annamalai, Inga-Marie Pompös, Bärbel Rohrer, Florian Sennlaub, Nadine Reichhart, Gerhild Wildner & Olaf Strauß
J Neuroinflammation 19, 260 (2022). https://doi.org/10.1186/s12974-022-02620-w
Generation of induced pluripotent stem cell lines from three patients with Aicardi-Goutières syndrome type 5 due to biallelic SAMDH1 mutations
Vanessa Hänchen, Stefanie Kretschmer, Christine Wolf, Kerstin Engel, Shahryar Khattak, Katrin Neumann and Min Ae Lee-Kirsch
Stem Cell Res. 2022 October;64:102912.
Generation of induced pluripotent stem cell lines from two patients with Aicardi-Goutières syndrome type 1 due to biallelic TREX1 mutations
Vanessa Hänchen, Stefanie Kretschmer, Christine Wolf, Kerstin Engel, Shahryar Khattak, Katrin Neumann and Min Ae Lee-Kirsch
Stem Cell Res. 2022 October;64:102895.
Human photoreceptor cell transplants integrate into human retina organoids
Felix Wagner, Roberto Carrera, Thomas Kurth, Stylianos Michalakis, Ronald Naumann, Marta Zuzic, Katrin Neumann, Olivier Gourau, Volker Busskamp and Mike O. Karl
bioRxiv 2022.08.09.500037; doi: https://doi.org/10.1101/2022.08.09.50003
Transplanted human cones incorporate into the retina and function in a murine cone degeneration model
Sylvia J. Gasparini, Karen Tessmer, Miriam Reh, Stephanie Wieneke, Madalena Carido, Manuela Völkner, Oliver Borsch, Anka Swiersy, Marta Zuzic, Olivier Goureau, Thomas Kurth, Volker Busskamp, Günther Zeck, Mike O. Karl and Marius Ader
J Clin Invest., 2022 April 28; 132(12):e154619.
FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes
Francesca Murganti, Wouter Derks, Marion Baniol, Irina Simonova, Palina Trus, Katrin Neumann, Shahryar Khattak, Kaomei Guan and Olaf Bergmann
Front. Cardiovasc. Med., 2022 April 25. https://doi.org/10.3389/fcvm.2022.840147
Correction of a Factor VIII genomic inversion with designer-recombinases
Felix Lansing, Liliya Mukhametzyanova, Teresa Rojo-Romanos, Kentaro Iwasawa, Masaki Kimura, Maciej Paszkowski-Rogacz, Janet Karpinski, Tobias Grass, Jan Sonntag, Paul Martin Schneider, Ceren Günes, Jenna Hoersten, Lukas Theo Schmitt, Natalia Rodriguez-Muela, Ralf Knöfler, Takanori Takebe and Frank Buchholz
Nature Commun. 2022 Jan 20; 13: 422.
2020
A Human Retinal Pigment Epithelium-Based Screening Platform Reveals Inducers of Photoreceptor Outer Segments Phagocytosis
Sven Schreiter, Katerina Vafia, Rico Barsacchi, Stephen H. Tsang, Marc Bickle, Marius Ader, Mike O. Karl, Elly M. Tanaka and Seba Almedawar
Stem Cell Reports. 2020 Dec 8; 15(6): 1347–1361.
Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors
Duran Sürün, Aksana Schneider, Jovan Mircetic, Katrin Neumann, Felix Lansing, Maciej Paszkowski-Rogacz, Vanessa Hänchen, Min Ae Lee-Kirsch and Frank Buchholz
Genes (Basel). 2020 May 6;11(5):E511.
Generation of human iPS cell line IBCHi002-A from spinocerebellar ataxia type 3/Machado-Joseph disease patient's fibroblasts
Agata Ciolak, Wlodzimierz J. Krzyzosiak, Emilia Kozlowska, Agnieszka Fiszer
Stem Cell Res. 2020 Apr 20;45:101796.
MERTK-Dependent Ensheathment of Photoreceptor Outer Segments by Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium
Seba Almedawar, Katerina Vafia, Sven Schreiter, Katrin Neumann, Shahryar Khattak, Thomas Kurth, Marius Ader, Mike O.Karl, Stephen H.Tsang and Elly M.Tanaka
Stem Cell Reports. 2020 Mar 10;14(3):374-389.
Inhibition of Ca2+ channel surface expression by mutant bestrophin-1 in RPE cells.
Magdalena Cordes, Piotr Bucichowski, Ahmad S. Alfaar, Stephen H. Tsang, Seba Almedawar, Nadine Reichhart and Olaf Strauß
FASEB J. 2020 Mar;34(3):4055-4071.
2019
Organoid single-cell genomic atlas uncovers human-specific features of brain development.
Sabina Kanton, M. J. Boyle, Z. He, M. Santel, A. Weigert, F. Sanchís-Calleja, P. Guijarro, L. Sidow, J. S. Fleck, D. Han, Z. Qian, M. Heide, W. B. Huttner, P. Khaitovich, S. Pääbo, B. Treutlein and G. Camp
Nature. 2019 Oct;574(7778):418-422.
Comparative RNAi Screens in Isogenic Human Stem Cells Reveal SMARCA4 as a Differential Regulator.
Ceren Güneş, Maciej Paszkowski-Rogacz, Susann Rahmig, Shahryar Khattak, Aylin Camgöz, Martin Wermke, Andreas Dahl, Martin Bornhäuser, Claudia Waskow and Frank Buchholz
Stem Cell Reports. 2019 May 14;12(5):1084-1098.
2018
Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.
Lisa K. Kutsche, Deisy M. Gysi, Joerg Fallmann, Kerstin Lenk, Rebecca Petri, Anka Swiersy, Simon D. Klapper, Karolina Pircs, Shahryar Khattak, Peter F. Stadler, Johan Jakobsson, Katja Nowick and Volker Busskamp
Cell Syst. 2018 Oct 24;7(4):438-452.e8.