Seniorprofessur Bioverfahrenstech­nik

Die Bioverfahrenstechnik versteht sich als Brücke zwischen molekularbiologischer und mikrobiologischer Grundlagenforschung und der Realisierung biotechnischer Stoffumwandlungen im industriellen Maßstab. Die Nutzung von Naturstoffen (Weiße Biotechnologie) steht im Zentrum des Interesses. Mit Methoden zum schnellen Biomonitoring (Flow Cytometry, Magnetseparation in Kombination mit bildgebenden Verfahren, Biosensoren) werden zeitnah Informationen aus Zellfabriken gewonnen, auf deren Grundlage Stoffwechsel- und Prozessmodelle zur Beschreibung und Vorhersage biotechnischer Prozesse und damit zur Maßstabsübertragung genutzt werden können.

Portraitfoto von Professor Bley © Christian Hüller

Seniorprofessor für Bioverfahrenstechnik

Name

Prof. Dr. rer. nat. habil. Thomas Bley

work Tel.
+49 351 463-32420
fax Fax
+49 351 463-37761

Kontakt über Sekretariat: Heike Pfennig

Details

Akademische Ausbildung

  • Mathematikstudium an der Technischen Universität Dresden (bis 1975)
  • Promotion über "Strukturierte Wachstumsmodelle - Vergleichende Untersuchungen und Anwendung auf Probleme der technischen Mikrobiologie" (1981)
  • Habilitation zum Thema "Zustandsstrukturierte Modelle in der Biotechnologie - Anwendung von Delay-Differentialgleichungen zur Beschreibung der Dynamik mikrobieller Populationen" (1990) 

Berufliche Stationen

  • Wissenschaftlicher Mitarbeiter am Institut für Biotechnologie (IBT) der Akademie der Wissenschaften der DDR in Leipzig (1975 - 1990)
  • Leiter der Abteilung Biosignale am IBT (1990 - 1991)
  • Leiter des Projektes Biosignale im Wissenschaftler-Integrationsprogramm (1992 - 1993)
  • Leiter der Abteilung Biotechnologie an der Fakultät für Biowissenschaften, Pharmazie und Psychologie der Universität Leipzig (1994 - 1996)
  • Professor für Bioverfahrenstechnik an der Technischen Universität in Dresden (seit 1996)
  • Seniorprofessor für Bioverfahrenstechnik an der Technischen Universität in Dresden (seit 2017)

Mitgliedschaften

  • Ordentliches Mitglied der Sächsischen Akademie der Wissenschaften, Klasse Technikwissenschaften
  • Ordentliches Mitglied der deutschen Akademie der Technikwissenschaften - acatech
  • Mitglied des DFG-Fachkollegiums "Verfahrenstechnik, Technische Chemie"
  • Vorsitzender der Beirates der GVC-DECHEMA Fachgruppe Bioprozesstechnik
  • Mitglied des Vorstandes der DECHEMA-Fachgemeinschaft Biotechnologie
  • Editor-in-Chief des Wiley-VCH Journals "Engineering in Life Sciences"

Forschungsschwerpunkte

  • Biomonitoring und Biosignale (Flow Cytometry)
  • Modellierung und Steuerung von Bioprozessen
  • Biokatalyse in heterogenen Systemen
  • Weiße Biotechnologie
  • Pflanzenzellen in Bioreaktoren
Publikationen

Henseleit A, Pohl C, Bley T, Boschke E (2015) Monitoring human serum albumin cell culture processes using Surface Plasmon Resonance (SPR) spectroscopy. J. Sens. Sens. Syst. 4, 77–83

Henseleit A, Pohl C, Kaltenbach H-M, Hettwer K, Simon K, Uhlig S, Haustein N, Bley T, Boschke E (2015) Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System. Biosensors 5, 27–36

Jost B, Holz M, Aurich A, Barth G, Bley Th, Müller R A (2015) The Influence of Oxygen Limitation for the Production of Succinic Acid with Recombinant Strains of Yarrowia lipolytica. Appl. Microbiol. Biotechnol. 99 (4), 1675-1686

Krujatz F, Härtel P, Helbig K, Haufe N, Thierfelder S, Bley T, Weber J (2015) Hydrogen production by Rhodobacter sphaeroides DSM 158 under intense irradiatio. Bioresource Technology 175, 82–90

Krujatz F, Lode A, Brüggemeier S, Schütz K, Kramer J, Bley T, Gelinsky M, Weber J (2015) Green bioprinting: Viability and growth analysis of microalgae immobilized in 3D-plotted hydrogels versus suspension cultures. Eng. Life Sci.

Krujatz F, Illing R, Krautwer T, Liao J, Helbig K, Goy K, Opitz J, Cuniberti G, Bley Th, Weber J (2015) Light-field-characterization in a continuous hydrogen-producing photobioreactor by optical simulation and computational fluid dynamics. Biotechnology and Bioengineering

Lenk F, Bley T (2015) Ramified Challenges: Monitoring and Modeling of Hairy Root Growth in Bioprocesses — A Review. Advances in Biochemical Engineering & Biotechnology, 1-21

Lode A, Krujatz F, Brüggemeier S, Quade M, Schütz K, Knaack S, Weber J, Bley Th, Gelinsky M(2015) Green bioprinting: Fabrication of photosynthetic algae-laden hydrogel scaffolds for biotechnological and medical applications. Eng. Life Sci. 15, 177-181

Löser C, Urit T, Keil P, Bley T (2015) Studies on the mechanism of synthesis of ethyl acetate in Kluyveromyces marxianus DSM 5422. Applied Microbiology and Biotechnology 99, 1131-1144

Löser C, Urit T, Gruner E, Bley T (2015) Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate. Energy Sustainability and Society 5, 1-15

Ludwig B, Geib D, Haas C, Steingroewer J, Bley Th, Muffler M, Ulber R (2015)Whole-cell biotransformation of the triterpene oleanolic acid by free and immobilized cells of Nocardia iowensis: Characterization of new metabolitesEng. Life Sci. 15, 108-115

Maschke R W, Geipel K, Bley T (2015) Modeling of Plant in Vitro Cultures: Overview and Estimation of Biotechnological Processes. Biotechnology and Bioengineering 112, 1-12

Steudler S, Bley Th (2015) Biomass estimation during macro-scale solid-state fermentation of basidiomycetes using established and novel approaches. Advances in Biochemical Engineering Biotechnology Bioprocess Biosyst. Eng. 38, 1313-1323

Steudler S, Bley Th (2015) Better one-eyed than blind – Challenges and opportunities of biomass measurement during solid-state fermentation of basidiomycetes. Advances in Biochemical Engineering Biotechnology 149, 223-252

Steudler S, Boehmer U, Weber J (2015) Biomass measurement by flow cytometry during solid-state fermentation of basidiomycetes. Cytometry A 87, 176-88

Vogel M, Boschke E, Bley T, Lenk F (2015) PetriJet Platform Technology: An Automated Platform for Culture Dish Handling and Monitoring of the Contents. Journal of Laboratory Automation, 1-10

Geipel K, Song X, Socher M L, Kümmritz S, Püschel J, Bley T, Ludwig-Müller J, Steingroewer J (2014) Induction of a photomixotrophic plant cell culture of Helianthus annuus and optimization of culture conditions for improved alpha-tocopherol production. Applied Microbiology and Biotechnology, 98(5):2029-2040

Georgiev V, Schumann A, Pavlov A, Bley Th (2014) Temporary Immersion Systems in Plant Biotechnologies. Eng. Life Sci. 14, 607-621

Haas C, Hengelhaupt KC, Kümmritz S, Bley Th, Pavlov A, Steingroewer J (2014) Salvia suspension cultures as production systems for oleanolic and ursolic acid. Acta Physiologica Plantarum 36, 2137-2147

Kümmeritz S, Haas C, Pavlov A, Geib D, Ulber R, Bley Th, Steingroewer J (2014) Determination of triterpenic acids and screening for valuable secondary metabolites in Salvia sp. suspension cultures. Natural Product Communications 9, 17-20

Löser C, Urit T, Bley T (2014) Perspectives for the biotechnological production of ethyl acetate by yeasts. Applied Microbiology and Biotechnology 98, 5397-5415.

Marchev, A., Haas, C., Schulz, S., Georgiev, V., Steingroewer, J., Bley, Th., Pavlov, A. (2014) Biotechnology of Sage: a promising tool for production of bioactive terpenes and phenolic substances. Biotechnology Letters, 36: 211-221

Mardawati E, Werner A, Bley Th, Kresnowati M, Setiadi T (2014) The enzymatic hydrolysis of oil palm empty fruit bunches to xylose. Journal of the Japan Institue of Energy 93, 973-978

Socher ML, Lenk F, Geipel K, Schott C, Püschel J, Haas C, Grasse C, Bley T, Steingroewer J (2014) Phototrophic growth of Arthrospira platensis in a respiration activity monitoring system for shake flasks (RAMOS). Engineering in Life Sciences, 14(6):658-666.

Wagner, K., Friedrich, S., Stang, C., Bley, Th., Schilling, N., Bieda, M., Lasagni, A., Boschke, E. (2014) Initial phases of microbial biofilm formation on opaque, innovative anti-adhesive surfaces using a modular microfluidic system. Engineering in Life Sciences, 14(1):76-84

Weber J, Krujatz F, Hilpmann G, Grützner S, Herrmann J, Thierfelder S, Bienert G, Illing R, Helbig K, Hurtado A, Cuniberti G, Mertig M, Lange R, Günther E, Opitz J, Lippmann W, Bley T, Haufe N (2014) Biotechnological hydrogen production by photosynthesis. Engineering in Life Sciences, 14(6):592-606

Werner S, Greulich J, Geipel K, Steingroewer J, Bley T, Eibl D (2014) Mass propagation of Helianthus annuus suspension cells in orbitally shaken bioreactors: Improved growth rate in single-use bag bioreactors. Engineering in Life Sciences, 14(6):676-684

Wagner K, Friedrich S, Bley T, Schilling N, Boschke E (2013) Modular microfluidic system for the investigation of adhesion and microbial biofilm formation on non-transparent surfaces. Eng Life Sci., DOI: 10.1002/elsc.201200035

Geipel K, Socher M L, Haas C, Bley T, Steingroewer J (2013) Growth kinetics of a Helianthus annuus and a Salvia fruticosa suspension cell line: Shake flask cultivations with online monitoring system. Engineering in Life Sciences, 13:593-602

Urit T, Manthey R, Bley T, Löser C (2013) Formation of ethyl acetate by Kluyveromyces marxianus on whey: influence of aeration and inhibition of yeast growth by ethyl acetate. Eng. Life Sci. 13(3) 247-260

Moeller L, Zehnsdorf A, Aurich A, Barth G, Bley T, Strehlitz B (2013) Citric acid production from sucrose by recombinant Yarrowia lipolytica using semicontinuous fermentation. Eng. Life Sci. 13(2) 163-171

Steingroewer J, Bley T, Georgiev V, Ivanov I, Lenk F, Marchev A, Pavlov A (2013) Review: Bioprocessing of differentiated plant in vitro systems. Eng. Life Sci., 13(1), 26-38

Moeller L, Zehnsdorf A, Aurich A, Bley T, Strehlitz B (2012) Substrate utilization by recombinant Yarrowia lipolytica growing on sucrose. Applied Microbiology and Biotechnology 93, 1695-1702

Kottmeier K, Günther T, Weber J, Kurtz S, Ostermann K, Rödel G, Bley T (2012) Surface active hydrophobin HFB1 from Trichoderma reesei expressed in Pichia pastoris pre-purified using foam separation during cultivation. Eng. Life Sci. 12, 162-170

Urit T, Löser C, Stukert A, Bley T (2012) Formation of ethyl acetate by Kluyveromyces marxianus on whey during aerobic batch cultivation at specific trace-element limitation. Applied Microbiology and Biotechnology 96, 1313-1323

Lenk F, Vogel M, Bley T, Steingroewer J (2012) Determining Morphological Development and Secondary Metabolite Accumulation in Hairy Root Networks. Eng. Life Sci., 12(6), 588-594

Mauermann M, Bellmann C, Calvimontes A, Caspari A, Bley T, Majschak JP (2012) Reinigbarkeit von Oberflächen in der Lebensmittelindustrie durch Flüssigkeitsstrahlen. Chemie Ingenieur Technik 84, 1568-1574

Löser C, Urit T, Förster S, Stukert A, Bley T (2012) Formation of ethyl acetate by Kluyveromyces marxianus on whey during aerobic batch and chemostat cultivation at iron limitation Applied Microbiology and Biotechnology 96, 685-696

Günther S, Koch C, Hübschmann T, Röske I, Müller R, Bley T, Harms H, Müller S (2012) Correlation of community dynamics and process parameters as a tool for the prediction of the stability of wastewater treatment Environmental Science & Technology 46, 84-92

Boschke E, Henseleit A, Schmieder S, Bley T, Sonntag F, Schilling N, Quenzel P, Danz N, Klotzbach U (2011) A compact and rapid aptasensor platform based on surface plasmon resonance. Eng. Life Sci. 11, 573-579

Georgiev V, Marchev A, Haas C, Weber J, Nikolova M, Pavlov A (2011) Production of oleanolic and ursolic acids by callus culture of Salvia tomentosa Mill. Biotechnology and Biotechnological Equipment 25, 4 Suppl. 34-38

Boehmer U, Frömmel S, Bley T, Müller M, Frankenfeld K, Miethe P (2011) Solid-state fermentation of lignocellulotic materials for the production of enzymes by the white-rot fungus, Trametes hirsuta, in a modular bioreactor. Eng. Life Sci. 11, 395-401


Trutnau M, Petzold M, Mehlig L, Eschenhagen M, Geipel K, Müller S, Bley T, Röske I (2011) Using a carbon based ASM3 EAWAG Bio-P for modelling the enhanced biological phosphorus removal (EBPR) in anaerobic/aerobic activated sludge systems. Bioproc. Biosyst. Engin. 34, 287-295

Stancheva N, Weber J, Schulze J, Alipieva K, Ludwig-Müller J, Haas C, Georgiev V, Bley T, Georgiev M (2011) Phytochemical and flow cytometric analyses of Devil´s claw cell cultures Plant Cell Tissue and Organ Culture 105, 79-84

Walther T, Reinsch H, Ostermann K, Deutsch A, Bley T (2011) Applying Dimorphic Yeasts as Model Organisms to Study Mycelial Growth: Part 2: Application of Mathematical Models to Identify Different Construction Principles in Yeast Colonies. Bioprocess and Biosystems Engineering, 34, 21-31

Walther T, Reinsch H, Weber P, Ostermann K, Deutsch A, Bley T (2011) Applying Dimorphic Yeasts as Model Organisms to Study Mycelial Growth: Part 1: Experimental Investigation of the Spatio-temporal Development of Filamentous Yeast Colonies. Bioprocess and Biosystems Engineering, 34, 13-20

Ed.: Müller S and Bley T (2011) High Resolution Microbial Single Cell Analytics. Series: Advances in Biochemical Engineering/Biotechnology, Vol. 124, Springer-Verlag Berlin Heidelberg, ISBN-Print: 978-3-642-16886-4

Kottmeier K, Ostermann K, Bley T, Rödel G (2011) Hydrophobin signal sequence mediates efficient secretion of recombinant Proteins in Pichia pastoris. Applied Microbiology and Biotechnology 91, 133-141

Löser C, Urit T, Nehl F, Bley T (2011) Screening of Kluyveromyces strains for the production of ethyl acetate: Design and evaluation of a cultivation system. Engineering in Life Sciences, DOI: 10.1002/elsc.201000178

Moeller L, Grünberg M, Zehnsdorf A, Aurich A, Bley T, Strehlitz B (2011) Repeated Fed-Batch Fermentation Using Biosensor online Control for Citric Acid Production by Yarrowia lipolytica. Journal of Biotechnology 153, 133–137

Gyurkovska V, Alipieva K, Maciuk A, Dimitrova P, Ivanovska N, Haas C, Bley T, Georgiev M (2011) Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents. Food Chemistry 125, 171-178

Muffler K, Leipold D, Scheller MC, Haas C, Steingroewer J, Bley T, Neuhaus HE, Mirata MA, Schrader J, Ulber R (2011) Biotransformation of triterpenes. Process Biochemistry 40, 1-15

Urit T, Löser C, Wunderlich M, Bley T (2011) Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping. Bioproc. Biosyst. Engin. 34, 547–559

Georgiev M, Ludwig-Müller J, Weber J, Stancheva N, Bley T (2011) Bioactive Metabolite Production and Stress-related Hormones in Devil’s Claw Cell Suspension Cultures Grown in Bioreactors. Applied Microbiology and Biotechnology 89, 1683-1691

Bley T (2011) From single cells to microbial population dynamics: Modelling in biotechnology based on measurements of individual cells. Advances in Biochemical Engineering Biotechnology 124, 211-227

Weber J, Georgiev V,  Haas C,  Bley T,  Pavlov A (2010) Ploidy levels in Beta vulgaris (red beet) plant organs and in vitro systems. Engineering in Life Sciences, 10, 139-147

Böhmer U, Kirsten C, Bley T, Noack M (2010) Use of lignite granules and lignitic xylite combined with white rot fungi as a novel method to treat wastewater from the textile industry. Engineering in Life Sciences, 10, 26-34

Frömmel S, Böhmer U, Bley Th, Müller M, Frankenfeld K, Miethe P (2010) Feststofffermentation von Lignocellulose-Reststoffen mittels Weißfäulepilzen in einem Bioreaktor mit modularem Design. Chemie Ingenieur Technik 82 (8), 1191-1196. DOI: 10.1002/cite.201000047

Berkov S, Pavlov A, Georgiev V, Weber J, Bley T, Viladomat F, Bastida J, Codina C (2010) Changes in apolar metabolites during in vitro organogenesis of Pancratium maritimum. Plant Physiology and Biochemistry 48, 827-835. DOI: 10.1016/j.plaphy.2010.07.002

Homova V, Weber J, Schulze J, Alipievac K, Bley T, Georgiev M (2010) Devil’s Claw Hairy Root Culture in Flasks and in a 3-L Bioreactor: Bioactive Metabolite Accumulation and Flow Cytometry. Zeitschrift für Naturforschung C 65(7-8): 472-478

Trutnau M, Bley T, Ondruschka J (2010) Chitosan from fungi, In: Chitosan: Manufacture, Properties, and Usage, Ed. Davis SP, Nova Science Publishers, New York 

Moeller L, Grünberg M, Zehnsdorf A, Strehlitz B, Bley T (2010) Biosensor Online Control of Citric Acid Production from Glucose by Yarrowia lipolytica Using Semicontinuous Fermentation. Engineering in Life Sciences, 10, 311-320. DOI: 10.1002/elsc.200900073

Walther T, Reinsch H, Weber Ph, Ostermann K, Deutsch A, Bley T (2010) Applying Dimorphic Yeasts as Model Organisms to Study Mycelial Growth: Part 1: Experimental Investigation of the Spatio-temporal Development of Filamentous Yeast Colonies. Bioprocess and Biosystems Engineering, 34, 13-20. DOI: 10.1007/s00449-010-0442-6

Walther T, Reinsch H, Ostermann K, Deutsch A, Bley T (2010) Applying Dimorphic Yeasts as Model Organisms to Study Mycelial Growth: Part 2: Application of Mathematical Models to Identify Different Construction Principles in Yeast Colonies. Bioprocess and Biosystems Engineering, 34, 21-31. DOI: 10.1007/s00449-010-0443-5

Leipold D, Wünsch G, Schmidt M, Bart HJ, Bley T, Neuhaus HE, Bergmann H, Richling E, Muffler K, Ulber R (2010) Biosynthesis of ursolic acid derivatives by microbial metabolism of ursolic acid with Nocardia sp. Strains – proposal of new biosynthetic pathways. Process Biochemistry 45 (7) 1043-1051, DOI: 10.1016/j.procbio.2010.03.013

Müller S, Bley T (2010) Flow Cytometry, In: Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology Vol. 7, Ed. Flickinger MC, Wiley, New York, 2451-2467

Georgiev M, Alipieva K, Pashova S, Denev P, Angelova M, Kerns G, Bley T (2010) Antioxidant Activity of Devil’s Claw Cell Biomass and its active Constituents. Food Chemistry 121 (4), 967-972, DOI: 10.1016/j.foodchem.2010.01.028

Pavlov A, Weber J, Georgiev V, Bley T (2010) Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. Detroit Dark Red. Plant Foods for Human Nutrition, DOI 10.1007/s11130-010-0156-6

Müller S, Harms H, Bley T (2010) Origin and analysis of microbial population heterogeneity in bioprocesses. Current Opinion in Biotechnology 21 (1), 100-113

Weber J, Georgiev V, Haas C,  Bley T,  Pavlov A (2010) Ploidy levels in Beta vulgaris (red beet) plant organs and in vitro systems. Engineering in Life Sciences 10(1), 1–9

Böhmer U, Kirsten C, Bley T, Noack M (2010) White-rot fungi combined with lignite granules and lignitic xylite to decolorize textile industry wastewater. Engineering in Life Sciences 10(1), 26–34

Lanfer B, Seib FP, Freudenberg U, Stamov D, Bley T, Werner C (2009) The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices. Biomaterials 30 (30), 5950-5958, DOI: 10.1016/j.biomaterials.2009.07.039

Kottmeier K*,Weber J*, Müller C, Bley T, Büchs J (2009) Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation. Biotechology and Bioengeneering, DOI: 10.1002/ bit.22410 (*equal contribution)

Pavlov A, Berkov S, Weber J, Bley T (2009) Hyoscyamine biosynthesis in Datura stramonium Hairy root in vitro systems with different ploidy levels. Appl. Biochem. Biotechnol., 157, 210-225

Trutnau M, Suckale N, Groeger G, Bley T, Ondruschka J (2009) Enhanced chitosan production and modeling hyphal growth of Mucor rouxii interpreting the dependence of chitosan yields on processing and cultivation time. Engineering in Life Sciences 9, 437-443

Bley, T. (Hrsg.) Biotechnologische Energieumwandlung - Gegenwärtige Situation, Chancen und künftiger Forschungsbedarf. acatech DISKUTIERT, Springer-Verlag Berlin, Heidelberg 2009

Stottmeister U, Bley Th (2009) Forschung zur Technikbewertung und -gestaltung in der Denkströme, Journal der Sächsischen Akademie der Wissenschaften, Leipziger Universitätsverlag ISSN 1867-6413, 219-226

Georgiev V, Weber J, Bley T, Pavlov A (2009) Improved procedure for nucleus extraction for DNA measurements by flow cytometry of red beet (Beta vulgaris L.) hairy roots. Journal of Bioscience and Bioengineering (107), 439-441

Günther S, Trutnau M, Kleinsteuber S, Bley T,  Röske I, Harms H, Müller S (2009) Dynamics of polyphosphate accumulating bacteria in waste water treatment plant communities detected via DAPI (4´,6´-Diamidino-2-Phenylindole) and tetracycline labeling. Appl. Environ. Microbiol. (75), 2111-2121

Mauermann M, Eschenhagen U, Bley T, Majschak JP (2009) Surface Modifications – Application Potential for the Reduction of Cleaning Costs in the Food processing Industry. Trends in Food Science and Technology 20, 8-15

Hortsch R, Löser C, Bley T (2008) A two-stage CSTR cascade for studying the effect of inhibitory and toxic substances in bioprocesses. Engineering in Life Sciences (6), 650-657

Georgiev V, Stukert A, Bley T, Pavlov A (2008) Hyosciamine biosynthesis by diploid and tetraploid Datura stramonium L. hairy root cultures in a temporary immersion cultivation system. Advances in Bulgarian Sciences (2-3), 42-47

Mauermann M, Majschak JP, Eschenhagen U, Bley Th (2008) Reduzierung der Reinigungskosten: Modifizierte Oberflächen verringern Verschmutzungen. Journal für Oberflächentechnik (48), 70-74

Weber J, Georgiev V, Pavlov A, Bley T (2008) Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger. Cytometry A (73A), 931-93

Haas C, Weber J, Müller-Ludwig J, Deponte S, Bley T, Georgiev MI (2008) Flow cytometry and phytochemical analysis of a sunflower cell suspension culture in a 5-L bioreactor. Zeitschrift für Naturforschung (63), 699-705

Georgiev MI, Georgiev V, Weber J, Bley T, Ilieva M, Pavlov AI (2008) Agrobacterium rhizogenes-mediated genetic transformations: Powerful tool for metabolites production. In: Genetically Modified Plants, Ed. T. V. Wolf & J. P. Koch, Nova Science Publishers

Ondruschka J, Trutnau M, Bley T (2008) Gewinnung und Potentiale des Biopolymers Chitosan. Chemie Ingenieur Technik, Chemie Ingenieur Technik 80(6), 811-818

Georgiev V, Pavlov A, Ilieva M, Bley T (2008) Betalains production in plant in vitro systems. Acta Physiologiae Plantarum, DOI 10.1007/s11738-008-0170-6

Bertau M, Katzberg M, Bley T (2008) Mit einer Tasse Kaffee zu neuen Wirkstoffen Chirale pharmazeutische Intermediate in höherer Stereoisomerenreinheit. BioForum 1, 49-51

Müller S, Bley T (2008) Flow Cytometry for Bioprocess Monitoring - Applications for Procaryotes and cell Cultures. Wiley Encyclopedia of Industrial Biotechnology

Eschenhagen U, Eschenhagen M, Ludwig F, Kiessling A, Symank K, Boschke E, Röske I, Bley T (2008) In situ hybridization of microcolonies using catalyzed reporter deposition with Tetramethylbenzidine – a method for detecting low numbers of bacterial cells in drinking water. European Food Research and Technology

Noack S, Klöden W, Bley T (2007) Modeling synchronous growth of bacterial populations in phased cultivation. Bioprocess and Biosystems Engineering, DOI: 10.1007/s00449-007-0180-6

Moeller L, Strehlitz B, Aurich A, Zehnsdorf A, Bley, Th (2007) Optimization of citric acid production from glucose by Yarrowia lipolytica. Engineering in Life Sciences 7, 504-511

Bohn M, Leppchen K, Katzberg M, Steingroewer J, Weber J, Bley T, Bertau M (2007) Effects of caffeine on stereoselectivities of high cell density biotransformations of cyclic ß-keto esters with Saccharomyces cerevisiae. Organic & Biomolecular Chemistry 5, 3456-3463

Pavlov AI, Georgiev MI, Bley T (2007) Batch and Fed-batch production of betalains by red beet (Beta vulgaris) hairy roots in a bubble column reactor. Zeitschrift für Naturforschung 62c, 439-446

Georgiev MI, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as source of bioactive substances. Applied Microbiology and Biotechnology 74, 1175-1185

Steingroewer J, Bley T, Bergemann C, Boschke E (2007) Biomegnetc separation of Salmonella typhimurium with high affine and specific ligand peptides isolated by Phage Display Technique. Journal of Magnetism and Magnetic Materials 311, 295-299

Starruß J, Bley T, Sogaar-Andersen L, Deutsch A (2007) A new mechanism for collective migration in Myxococcus xanthus. Journal of Statistical Physics 128, 269-286

Boschke E, Böhmer U, Lange J, Constapel M, Schellenträger M, Bley T (2007) The use of respirometric measurements to determine the toxicity of textile dyes in aqueous solution and after oxidative decolourisation processes. Chemosphere 67, 2163-2168

Zosel J, Enseleit U, Opitz T, Bley T, Guth U (2006) Einsatz hochempfindlicher Gassensoren für das Monitoring der Aktivität von Schimmelpilzkulturen. Der Versuchs- und Forschungsingenieur 39, 31-37

Bernhardt G, Bley T (2006) Kompetenzzentrum integrierte Naturstofftechnik - ZINT. Landtechnik 61, 65

Ondruschka J, Groeger G, Geyer W, Bley T (2006) Fermentative Herstellung von Chitosan aus Pilzmyzelien. Chemie Ingenieur Technik 78, 479-483

Pavlov A, Bley T (2006) Betalains biosynthesis by Beta vulgaris L. hairy root culture in a temporary immersion system. Process Biochemistry 41, 848-852

Pich A, Bhattacharya S, Adler H-JP, Wage T, Taubeberger A, Li Z, van Pee K-H, Böhmer U, Bley T (2006) Composite magnetic particles as carriers for laccase from Trametes versicolor. Macromolecular Biosciences 6, 301-310

Bernhardt G, Bley T (2006) Kompetenzzentrum integrierte Naturstofftechnik - ZINT. Landtechnik 61, 65

Weber J, Bühner J, Bley T, Göhde R (2006) Populationen überwachen - Durchflußzytometrisches Online-Monitoring zur Prozesssteuerung. BioTec (5-6), 32-33

Böhmer U, Suhardi SH, Bley T (2006) Decolorizing reactive textile dyes by white – rot fungi using temporary lift up immersion cultivation Eng. Life Sci. 6, 417-420

Georgiev M, Heinrich M, Kerns G, Bley T, Pavlov A (2006) Production of iridoids and phenolics by transformed Harpagophytum procumbens root cultures. Eng. Life Sci. 6, 593-596

Groeger G, Geyer W, Bley T, Ondruschka J (2006) Fermentative Herstellung von Chitosan aus Pilzmycelien. Chemie Ingenieur Technik 78 (4), 479-483

Onyango C, Bley T, Jacob A, Henle T, Rohm H (2006) Influence of incubation temperature and time on resistant starch type III formation from autoclaved and acid-hydrolysed cassava starch. Carbohydrate Polymers 66, 494-499

Steingroewer J, Bley T, Boschke E (2006) Schnellnachweis von Mikroorganismen, Biomagnetische Separation in einem neuen Testverfahren. LVT Lebensmittel-Industrie 51(1), 54-56

Fritsch M, Starruß J, Loesche A, Mueller S, Bley T (2005) Cell Cycle Synchronization of Ralstonia eutropha by Continuous Phasing Measured via Flow Cytometry. Biotechnol. Bioeng. 92, 635-642

Walther T, Reinsch H, Ostermann K, Deutsch A, Bley T (2005) Coordinated development of yeast colonies. A) Experimental analysis of the adaptation to different nutrient concentrations. Eng. Life Sci. 5, 115-124

Walther T, Reinsch H, Ostermann K, Deutsch A, Bley T (2005) Coordinated development of yeast colonies. B) Quantitative modeling of diffusion-limited growth. Eng. Life Sci. 5, 125-133

Pavlov A, Werner S, Ilieva M, Bley T (2005) Characteristic of Helianthus annus plant cell culture as a producer of immunologically active exopopolysaccharides. Eng. Life Sci. 5, 280-283

Löser C, Schröder A, Deponte S, Bley T (2005) Balancing the ethanol formation in continuous bioreactors with ethanol stripping. Eng. Life Sci. 5, 325-332

Pavlov A, Kovatcheva P, Tuneva D, Ilieva M, Bley T (2005) Radical Scavenging Activity and Stability of Betalains from Beta vulgaris hairy root culture in Simulated Conditions of Human Gastrointestinal Tract. Plant Foods for Human Nutrition 60, 43-47

Boschke E, Steingroewer J, Bley T (2005) Einsatz der Biomagnetischen Separation zur mikrobiologischen Qualitätskontrolle von Lebensmitteln. Chemie Ingenieur Technik 77(7), 912-919

Onyango C, Noetzold H, Ziems A, Hofmann T, Bley T, Henle T (2005) Digestibility and antinutrient properties of acidified and extruded maize-finger millet blend in the production of Uji. Food Science and Technology 38, 697-707

Steingroewer J, Knaus H, Bley T, Boschke E (2005) Rapid Method for Pre-Enrichment and Detection of Salmonella typhimurium. Eng. Life Sci. 5, 267-272

Steingroewer J, Boschke E, Bley T (2004) Biomagnetische Separation zur mikrobiologischen Qualitätskontrolle von Lebensmitteln. 12. Heiligenstätter Kolloquium:"Technische Systeme für Biotechnologie und Umwelt", Tagungsband, 225-236

Deponte S, Steingroewer J, Löser C, Boschke E, Bley T (2004) Biomagnetic separation of Escherichia coli by use of anion-exchange beads: measurement and modeling of the kinetics of cell-bead interactions. Analytical and Bioanalytical Chemistry 379 (3), 419-26

Achilles J, Müller S, Bley T, Babel W (2004) Affinity of single S.cerevisiae cells to 2-NDBglucose under changing substrate conditions. Cytometry 61A, 88-98

Fialova A, Boschke E, Bley T (2004) Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements. International Biodeterioration & Biodegradation 54, 69-76

Onyango C, Bley T, Raddatz H, Henle T (2004) Flavour compounds in backslop fermented uji (an East African sour porridge). European Food Research and Technology 218, 579-583 doi 10.1007/s00217-003-0870-5

Onyango C, Henle T, Hofmann T, Bley T (2004) Production of high energy density fermented uji using a commercial alpha-amylase or by single-screw extrusion. LWT - Food Science and Technology 37, 401-407

Onyango C, Henle T, Ziems A, Hofmann T, Bley T (2004) Effect of extrusion variables on fermented maize-finger millet blend in the production of uji. LWT - Food Science and Technology 37, 409-415

Onyango C, Noetzold H, Bley T, Henle T (2004) Proximate composition and digestibility of fermented and extruded uji from maize-finger millet blend. LWT - Food Science and Technology 37, 827-832

Walther T, Reinsch H, Große A, Ostermann K, Deutsch A, Bley T (2004) Mathematical modeling of regulatory mechanisms in yeast colony development. Journal of Theoretical Biology 229, 327-338

Boschke E, Eschenhagen U, Bley T (2003) Schnelle Detektion von Keimen mittels Fluoreszenz in situ Hybridisierung. Swiss Pharma 25 (5), 5-11

Bley T (2003) Bioverfahrenstechnik - Ein neuer Partner in der deutschen Verfahrenstechnik. Wissenschaftliche Zeitschrift der TU-Dresden 52:4, 53-57

Ondruschka J, Bley T (2003) Biosorption of environmetally relevant heavy metals on slected biomaterials for wastewater treatment. Engineering in Life Sciences 3, 215-218

Boschke E, Steingroewer J, Ripperger S, Klingner E, Bley T (2002) Biomonitoring by combination of immunomagnetic separation and direct epifluorescence filter technique. European Cells and Materials 3 (Suppl.2), 146-147

Walther T, Ostermann K, Listewnik H-F, Rödel G, Bley T (2002) Verfahren zur biotechnologischen Herstellung von Xylit. Patent-Nr: DE 10222373.4-42

Bley T, Müller S (2002) How should microbial life be quantified to optimise bioprocesses. Acta Biotechnologica 22, 401-409

Boschke E, Eschenhagen U, Bühner J, Bley T (2002) Lebensmittelverderbern und Kontaminanten auf der Spur. BIOforum 6, 472-475

Ondruschka J, Bley T (2002) Biosorption umweltrelevanter Schwermetalle an ausgewählten Biomaterialien als Grundlage für die Reinigung belasteter Abwässer. Chemie Ingenieur Technik 74, 500-504

Boschke E, Schünemann K, Bley T (2000) Enzymatische Probenaufbereitung für die nachfolgende Detektion von Mikroorganismen in ausgewählten Stoffsystemen aus dem Bereich der Lebensmittelindustrie. Lebensmittel- und Verpackungstechnik 45, 299-301

Hardjito L, Bley T (2000) A model for biopigment formation by Serratia marcescens biovar A2/6 in batch culture containing lactic acid and beef extract. Acta Biotechnologica 20, 75-81

Simon D, Müller R H, Große H J, Bley T, Babel W (2000) Response of Ralstonia eutropha JMP 134 to long-term exposure to toxic substrates in nutristat cultivation as indicated by on-line fluorescence measurements. Bioprocess Engineering 23, 1-10

Müller S, Bley T (1999) Flow Cytometry. In: The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation, M C Flickinger, S W Drew (Eds), 1226 - 1238, John Wiley, New York

Müller S, Hutter KJ, Bley T (1999) Flow cytometric characterization of physiological states of brewer's yeast in industrial processes In: Food microbiology and food safety into the next millenium. 191-195. Ed.: Tujitelaars et al., Ponsen et Looyen, Wageningen

Müller S, Bley T, Babel W (1999) Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry. Journal of Biotechnology 75, 81-97

Große-Uhlmann R, Bley T (1999) A modular approach to situation identification of the dynamics of bacterial populations synthesizing poly-ß-hydoxybutyrate. Bioprocess Engineering 21, 191-200

Boschke E, Bley T (1998) Growth patterns of yeast colonies depending on nutrient supply. Acta Biotechnologica 18, 17-27

Werner A, Boschke E, Bley T (1997) Immobilisierung fettspaltender Mikroorganismen zur Reinigung fetthaltiger Abwässer der Lebensmittelindustrie. Wissenschaftliche Zeitschrift der Technischen Universität Dresden 46, 33-39

Müller S, Hutter K J, Bley T, Petzold L, Babel W (1997) Dynamics of yeast cell states during proliferation and non proliferation periods in a brewing reactor monitored by multidimensional flow cytometry. Bioprocess Engineering 17, 287-293

Herrmann C, Lösche A, Müller S, Bley T, Babel W. (1997) Flow cytometric discrimination between Acinetobacter calcoaceticus 69-V and Alcaligenes eutrophus JMP 134 by fluorescently labelled rRNA-targeted oligonucleotide probes and DNA staining. Acta Biotechnolica 17, 19-38

Bley T, Albrecht K F, Miethe D, Riis V, Babel W (1996) Describing microbial degradation processes with the evolon model. Acta Biotechnolica 16, 237-244

Müller S, Loffhagen N, Bley T, Babel W (1996) Membrane-potential-related fluorescence intensity indicates bacterial injury. Microbiolical Research 151, 127-131

Simon D, Müller R H, Bley T, Babel W (1996) Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134. Bioprocess Engineering 14, 57-62

Bley T, Müller S, Lösche A, Ackermann J U, Babel W (1995) Flow cytometric monitoring of bacterial cell states under growth limiting conditions. In: Computer Applications in Biotechnology, A Munack, K Schügerl (Eds), Pergamon Press, Oxford, 213-216

Müller R H, Bley T, Babel W (1995) Transient state cultivation as a means for determining maximum growth rates in inhibition kinetics. Journal of Microbiolological Methods 22, 209-219

Miethe D, Albrecht K F, Riis V, Bley T, Babel W (1995) Description of the microbial degradation process for mineral oils and its extrapolation by the evolon model. In: Contaminated Soil ´95, W J van den Brink, R Bosman, F Arendt (Eds), Kluwer Academic Publishers, 1305-1306

Ackermann J U, Müller S, Lösche A, Bley T, Babel W (1995) Methylobacterium rhodesianum cells tend to double the DNA content under growth limitations and accumulate PHB. Journal of Biotechnology 39, 9-20

Müller S, Lösche A, Bley T, Scheper T (1995) A flow cytometric approach for characterization and differentiation of bacteria during microbial processes. Applied Microbiology and Biotechnology 43, 93-101

Lösche A, Müller S, Bley T (1994) Flow Cytometry - Charakterisierung von Bakterien. BIOforum 17, 56-60

Müller S, Lösche A, Bley T (1993) Staining procedures for flow cytometric monitoring of bacterial populations. Acta Biotechnolica 13, 289-297

Müller S, Lösche A, Bley T (1992) Flow cytometric investigation of sterol content and proliferation activity of yeast. Acta Biotechnologioca 12, 365-375

Bley T (1992) Delay-differential equations for modeling synchrony and periodic phenomena in microbial population dynamics. In: Modeling and control of biotechnical processes, M N Karim, G Stephanopoulos (Eds), Pergamon Press, 195-199

Bley T, Babel W (1992) Calculating affinity constants of substrate mixtures in a chemostat. Acta Biotechnologica 12, 13-15

Lösche A, Müller S, Stoll P, Bley T (1992) Durchflußzytometrie - eine physikalische Meßmethode in der Biotechnologie. Experimentelle Technische Physik 39, 97-102

Degelau A, Freitag R, Linz F, Middendorf C, Scheper T, Bley T, Müller S, Stoll P, Reardon K F (1992) Immuno- and flow cytometric analytical methods for biotechnological research and process monitoring. Journal of Biotechnololgy 25, 115-144

Möckel B, Bley T, Böhme B (1990) Model simulation of an efficient periodic control strategy for continuous fermentation processes. Acta Biotechnolica 10, 395-400

Bley T (1990) State structure models - A base for efficient control of fermentation processes. Biotechnology Advances 8, 233-259

Möckel B, Bley T, Böhme B (1989) Cyclic control of continuous biotechnological processes on the basis of a hierarchical control system. Systematic Analysis Modellation and Simulation 6, 181-196

Bley T, Wegner B (1988) Hopf bifurcation for a family of two-state microbial growth models. Acta Biotechnologica 8, 267-275

Möckel B, Böhme B, Bley T (1987) Optimal situation control for biosynthetic processes on the basis of a prediction filter. Acta Biotechnologica 7, 247-256

Bley T, Heinritz B (1987) Modelling and control of yeast growth in biotechnical systems. In: Dynamical Systems and Environmental Models, Akademie-Verlag Berlin, 213-219

Bley T (1987) State-structure models of microbial growth. Acta Biotechnologica 7, 173-177

Heinritz B, Bley T, Ringpfeil M (1985) Einsatz von Hochleistungsreaktoren zur mikrobiologischen Stoffwandlung. Chemie und Technik 37, 514-516

Heinritz B, Bley T (1985) State-models - An appropriate tool for improving the efficiency of biotechnical processes. In: Lotka-Volterra-Approach to Cooperation and Competition in Dynamical Systems, W Ebeling, M Peschel (Eds), Akademie-Verlag Berlin

Bley T, Heinritz B, Schmidt A (1984) Some stationary properties of a two-state microbial growth model for continuous fermentation derived from the Smith and Martin hypothesis. Studia Biophysica 98, 119-124

Bogner H, Bley T, Schneider K R (1984) Time optimal control for a growth model. Systematic Analysis Modellation and Simulation 1, 425-437

Heinritz B, Rogge G, Stichel E, Bley T (1983) Use of biorhytmic processes for increasing the efficiency of carbon-compound conversion by microorganisms. Acta Biotechnologica 3, 125-131

Heinritz B, Stichel E, Bley T, Glombitza F (1982) Theoretische Bestimmung energetischer Wirkungsgrade der mikrobiellen Kohlenstoffsubstratwandlung und Vergleich mit experimentellen Werten an Phasenkulturen. Zeitschrift für Allgemeine Mikrobiologie 22, 535-544

Stichel E, Rogge G, Bley T, Heinritz B (1982) Yield coefficients in dependence on milieu conditions and cell states. III. Induction of synchrony in continuous yeast cell cultivation by milieu changes. Zeitschrift für Allgemeine Mikrobiologie 22, 717-722

Heinritz B, Stichel E, Bley T, Rogge G, Glombitza F (1981) Yield coefficients in dependence on milieu conditions and cell states. II. Influence of pertubations on continuous cultivation of the yeast Lodderomyces elongisporus on hydrocarbons. Zeitschrift für Allgemeine Mikrobiologie 21, 583-588

Kuchenbecker D, Bley T, Schmidt A (1981) Short-periodic oscillations of culture fluorescence in yeast cell populations. stud biophysics 86, 157-162

Bley T, Bogner H (1981) Optimal control of a structured growth model. stud biophysics 83, 217-224

Bley T, Schmidt A (1980) A two state microbial growth model for continuous fermentation. Studia Biophysica 78, 11-12

Bley T, Heinritz B, Steudel A, Stichel E, Glombitza F, Babel W (1980) Yield coefficients in dependence on milieu conditions and cell states. I. Synchronized batch growth of a yeast. Zeitschrift für Allgemeine Mikrobiologie 20, 283-286

Heinritz B, Bley T (1979) Einfluß alternierender Störungen auf die Verbrauchskennziffern beim Wachstum von Mikroorganismen. Zeitschrift für Allgemeine Mikrobiologie 19, 247-252

Loffhagen N, Bley T, Babel W (1979) Regulation of pyruvate carboxylase from "hexulosephosphate pathway bacteria". Zeitschrift für Allgemeine Mikrobiologie 19, 367-372

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Mandy Schneider
Letzte Änderung: 15.03.2017