FACS Facility

The Institute of Physiological Chemistry has installed and operates a FACS facility to the benefit of the Dresden research community. The facility features four top-of-the-art machines:

two Becton Dickinson FACSAria sorter, one Becton Dickinson Symphony A3 analyzer and one Becton Dickinson LSRFortessa analyzer.


LOCATION

Institute for Physiological Chemistry, MTZ Building, Room B.10.001b (Symphony A3 + ARIA II),
Institute for Immunology, MTZ, Room B.10.026 (ARIA III),
Lab for Clinical Research at the Childrens clinics, UKD House 21, room 1.420 (LSRFortessa)


SYSTEM COMPONENTS

BD Symphony A3

5 lasers: 637 nm (red), 561nm (yellow-green), 488 nm (blue), 405 nm (violet), 355 nm (UV) 29 fluorescence and 1 side scatter detector, 1 forward scatter diode detector.

BD High Throughput Sampler for 96-well plates and 384-well-plates

BD FACSDiva v9.0 Software

BD FACSAriaII
   
          3 lasers: 633 nm (red), 488 nm (blue), 405 nm (violet)

          Auto-calibration and -compensation, automatic adjustment of drop-delay.
         
          10 fluorescence detectors and 1 side scatter detector, 1 forward scatter
          diode detector (12 parameters).
         
          Up to 4-way sorting (4 separate cell populations simultaneously).
          Up to 70.000 particle per second sortable (depending on parameters)

          Cell sorts into tissue culture plates (6-, 24-, 48-, 96- and 384-well) of defined
          numbers of events (from 1 cell until your well is full).
          Temperature-controlled collection device.

          3 nozzle sizes: 100 µm, 85 µm, 70 µm, which allow you to sort cells of different sizes.

BD FACSDiva v6.0 Software

BD FACSAriaIII
   
          4 lasers: 633 nm (red), 488 nm (blue), 405 nm (violet), 561 nm (yellow-green)

          Auto-calibration and -compensation, automatic adjustment of drop-delay.
         
          13 fluorescence detectors and 1 side scatter detector, 1 forward scatter
          diode detector (15 parameters).
         
          Up to 4-way sorting (4 separate cell populations simultaneously).
          Up to 70.000 particle per second sortable (depending on parameters)
          3 nozzle sizes: 100 µm, 85 µm, 70 µm, which allow you to sort cells of different sizes.

BD FACSDiva v9.0 Software

BD LSRFortessa

5 lasers: 640 nm (red), 561nm (yellow green) 488 nm (blue), 405 nm (violet), 355 nm (UV)

18 fluorescence and 1 side scatter detector, 1 forward scatter diode detector.

BD High Throughput Sampler for 96-well plates and 384-well-plates

BD FACSDiva v9.0 Software