FACS Facility
The Institute of Physiological Chemistry has installed and operates a FACS facility to the benefit of the Dresden research community. The facility features three top-of-the-art machines:
two Becton Dickinson FACSAria sorter and a Becton Dickinson LSR II analyzer.
LOCATION
Institute for Physiological Chemistry, MTZ Building, Room B.10.001b, Institute for Immunology, MTZ, Room B.10.026.
SYSTEM COMPONENTS
BD LSR II
4 lasers: 633 nm (red), 488 nm (blue), 405 nm (violet), 355 nm (UV)
12 fluorescence and 1 side scatter detector, 1 forward scatter diode detector.
RFP, GFP, YFP enabled
20.000 particles/second acquisition mode
BD FACSAriaII
3 lasers: 633 nm (red), 488 nm (blue), 405 nm (violet)
Auto-calibration and -compensation, automatic adjustment of drop-delay.
10 fluorescence detectors and 1 side scatter detector, 1 forward scatter
diode detector (12 parameters).
Up to 4-way sorting (4 separate cell populations simultaneously).
Up to 70.000 particle per second sortable (depending on parameters)
Cell sorts into tissue culture plates (6-, 24-, 48-, 96- and 384-well) of defined
numbers of events (from 1 cell until your well is full).
Temperature-controlled collection device.
3 nozzle sizes: 100 µm, 85 µm, 70 µm, which allow you to sort cells of different sizes.
BD FACSAriaIII
4 lasers: 633 nm (red), 488 nm (blue), 405 nm (violet), 561 nm (yellow-green)
Auto-calibration and -compensation, automatic adjustment of drop-delay.
13 fluorescence detectors and 1 side scatter detector, 1 forward scatter
diode detector (15 parameters).
Up to 4-way sorting (4 separate cell populations simultaneously).
Up to 70.000 particle per second sortable (depending on parameters)
3 nozzle sizes: 100 µm, 85 µm, 70 µm, which allow you to sort cells of different sizes.