Publikationen

Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, Jain G, Krüger J, Hermann PC, Schmid M, Madácsy T, Varga Á, Griger J, Azoitei N, Müller M, Wessely O, Robey PG, Heller S, Dantes Z, Reichert M, Günes C, Bolenz C, Kuhn F, Maléth J, Speier S, Liebau S, Sipos B, Kuster B, Seufferlein T, Rad R, Meier M, Hohwieler M, Kleger A. Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells. Cell Stem Cell. 2021 Jun 3;28(6):1105-1124.e19. doi: 10.1016/j.stem.2021.03.005. Epub 2021 Apr 28. PMID: 33915078

Huber MK, Drotar DM, Hiller H, Beery ML, Joseph P, Kusmartseva I, Speier S, Atkinson MA, Mathews CE, Phelps EA. Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices. J Vis Exp. 2021 Apr 12;(170):10.3791/62207. doi: 10.3791/62207. PMID: 33900291

Tang X, Kusmartseva I, Kulkarni S, Posgai A, Speier S, Schatz DA, Haller MJ, Campbell-Thompson M, Wasserfall CH, Roep BO, Kaddis JS, Atkinson MA. Image-Based Machine Learning Algorithms for Disease Characterization in the Human Type 1 Diabetes Pancreas. Am J Pathol. 2021 Mar;191(3):454-462. doi: 10.1016/j.ajpath.2020.11.010. Epub 2020 Dec 8. PMID: 33307036

Buitinga M, Cohrs CM, Eter WA, Claessens-Joosten L, Frielink C, Bos D, Sandker G, Brom M, Speier S, Gotthardt M. Noninvasive Monitoring of Glycemia-Induced Regulation of GLP-1R Expression in Murine and Human Islets of Langerhans. Diabetes. 2020 Nov;69(11):2246-2252. doi: 10.2337/db20-0616. Epub 2020 Aug 25. PMID: 32843570

Qadir MMF, Álvarez-Cubela S, Weitz J, Panzer JK, Klein D, Moreno-Hernández Y, Cechin S, Tamayo A, Almaça J, Hiller H, Beery M, Kusmartseva I, Atkinson M, Speier S, Ricordi C, Pugliese A, Caicedo A, Fraker CA, Pastori RL, Domínguez-Bendala J. Long-term culture of human pancreatic slices as a model to study real-time islet regeneration. Nat Commun. 2020 Jun 29;11(1):3265. doi: 10.1038/s41467-020-17040-8.PMID: 32601271

Panzer JK, Hiller H, Cohrs CM, Almaça J, Enos SJ, Beery M, Cechin S, Drotar DM, Weitz JR, Santini J, Huber MK, Muhammad Fahd Qadir M, Pastori RL, Domínguez-Bendala J, Phelps EA, Atkinson MA, Pugliese A, Caicedo A, Kusmartseva I, Speier S. Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. JCI Insight. 2020 Apr 23;5(8):134525. doi: 10.1172/jci.insight.134525.

Cohrs CM, Panzer JK, Drotar DM, Enos SJ, Kipke N, Chen C, Bozsak R, Schöniger E, Ehehalt F, Distler M, Brennand A, Bornstein SR, Weitz J, Solimena M, Speier S. Dysfunction of Persisting β Cells Is a Key Feature of Early Type 2 Diabetes Pathogenesis. Cell Rep. 2020 Apr 7;31(1):107469. doi: 10.1016/j.celrep.2020.03.033.

Panzer JK, Cohrs CM, Speier S. Using Pancreas Tissue Slices for the Study of Islet Physiology. Methods Mol Biol. 2020;2128:301-312. doi: 10.1007/978-1-0716-0385-7_20.

Cohrs CM, Chen C, Speier S. Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging. Methods Mol Biol. 2020;2128:149-157. doi: 10.1007/978-1-0716-0385-7_11.

Dudeck J, Froebel J, Kotrba J, Lehmann CHK, Dudziak D, Speier S, Nedospasov
SA, Schraven B, Dudeck A. Engulfment of mast cell secretory granules on skin
inflammation boosts dendritic cell migration and priming efficiency. J Allergy
Clin Immunol. 2018 Oct 17. pii: S0091-6749(18)31443-X. doi:
10.1016/j.jaci.2018.08.052. [Epub ahead of print] PubMed PMID: 30339853.

Weitz JR, Makhmutova M, Almaça J, Stertmann J, Aamodt K, Brissova M, Speier S, Rodriguez-Diaz R, Caicedo A. Mouse pancreatic islet macrophages use locally released ATP to monitor beta cell activity. Diabetologia. 2018 Jan;61(1):182-192.

Kemter E, Cohrs CM, Schäfer M, Schuster M, Steinmeyer K, Wolf-van Buerck L, Wolf A, Wuensch A, Kurome M, Kessler B, Zakhartchenko V, Loehn M, Ivashchenko Y, Seissler J, Schulte AM, Speier S, Wolf E. INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters. Diabetologia. 2017 Jun;60(6):1152-1156.

Chen C, Cohrs CM, Stertmann J, Bozsak R, Speier S. Human beta cell mass and function in diabetes: Recent advances in knowledge and technologies to understand disease pathogenesis. Mol Metab. 2017 Jul 8;6(9):943-957. 

Dudeck J, Medyukhina A, Fröbel J, Svensson CM, Kotrba J, Gerlach M, Gradtke AC, Schröder B, Speier S, Figge MT, Dudeck A. Mast cells acquire MHCII from dendritic cells during skin inflammation. J Exp Med. 2017 Dec4;214(12):3791-3811.

Cohrs CM, Chen C, Jahn SR, Stertmann J, Chmelova H, Weitz J, Bähr A, Klymiuk N, Steffen A, Ludwig B, Kamvissi V, Wolf E, Bornstein SR, Solimena M, Speier S. Vessel Network Architecture of Adult Human Islets Promotes Distinct Cell-Cell Interactions In Situ and Is Altered After Transplantation. Endocrinology. 2017 May 1;158(5):1373-1385.

Chen C, Chmelova H, Cohrs CM, Chouinard JA, Jahn SR, Stertmann J, Uphues I, Speier S. Alterations in β-Cell Calcium Dynamics and Efficacy Outweigh Islet Mass Adaptation in Compensation of Insulin Resistance and Prediabetes Onset. Diabetes. 2016 Sep;65(9):2676-85.

Anastasiou V, Ninou E, Alexopoulou D, Stertmann J, Müller A, Dahl A, Solimena M, Speier S, Serafimidis I, Gavalas A. Aldehyde dehydrogenase activity isnecessary for beta cell development and functionality in mice. Diabetologia. 2016 Jan;59(1):139-50.

Kragl M, Schubert R, Karsjens H, Otter S, Bartosinska B, Jeruschke K, Weiss J, Chen C, Alsteens D, Kuss O, Speier S, Eberhard D, Müller DJ, Lammert E. The biomechanical properties of an epithelial tissue determine the location of its vasculature. Nat Commun. 2016 Dec 20;7:13560.

Bader E, Migliorini A, Gegg M, Moruzzi N, Gerdes J, Roscioni SS, Bakhti M, Brandl E, Irmler M, Beckers J, Aichler M, Feuchtinger A, Leitzinger C, Zischka H,Wang-Sattler R, Jastroch M, Tschöp M, Machicao F, Staiger H, Häring HU, Chmelova H, Chouinard JA, Oskolkov N, Korsgren O, Speier S, Lickert H. Identification of proliferative and mature β-cells in the islets of Langerhans. Nature. 2016 Jul 21;535(7612):430-4.

Chmelova H, Cohrs CM, Chouinard JA, Petzold C, Kuhn M, Chen C, Roeder I, Kretschmer K, Speier S. Distinct roles of β-cell mass and function during type 1 diabetes onset and remission. Diabetes. 2015 Jun;64(6):2148-60.

Borg DJ, Weigelt M, Wilhelm C, Gerlach M, Bickle M, Speier S, Bonifacio E, Hommel A. Mesenchymal stromal cells improve transplanted islet survival and islet function in a syngeneic mouse model. Diabetologia. 2014 Mar;57(3):522-31.

Schubert N, Dudeck J, Liu P, Karutz A, Speier S, Maurer M, Tuckermann J, Dudeck A. Mast cell promotion of T cell-driven antigen-induced arthritis despite being dispensable for antibody-induced arthritis in which T cells are bypassed. Arthritis Rheumatol. 2015 Apr;67(4):903-13.

Marciniak A, Cohrs CM, Tsata V, Chouinard JA, Selck C, Stertmann J, Reichelt S, Rose T, Ehehalt F, Weitz J, Solimena M, Slak Rupnik M, Speier S. Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology. Nat Protoc. 2014 Dec;9(12):2809-22.

Ivanova A, Kalaidzidis Y, Dirkx R, Sarov M, Gerlach M, Schroth-Diez B, Müller A, Liu Y, Andree C, Mulligan B, Münster C, Kurth T, Bickle M, Speier S, Anastassiadis K, Solimena M. Age-dependent labeling and imaging of insulin secretory granules. Diabetes. 2013 Nov;62(11):3687-96.

Ilegems E, Dicker A, Speier S, Sharma A, Bahow A, Edlund PK, Leibiger IB, Berggren PO. Reporter islets in the eye reveal the plasticity of the endocrine pancreas. Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20581-6.

Marciniak A, Selck C, Friedrich B, Speier S. Mouse pancreas tissue sliceculture facilitates long-term studies of exocrine and endocrine cell physiology in situ. PLoS One. 2013 Nov 4;8(11):e78706.

Rodriguez-Diaz R, Speier S, Molano RD, Formoso A, Gans I, Abdulreda MH, Cabrera O, Molina J, Fachado A, Ricordi C, Leibiger I, Pileggi A, Berggren PO, Caicedo A. Noninvasive in vivo model demonstrating the effects of autonomic innervation on pancreatic islet function. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21456-61.

Nyqvist D, Speier S, Rodriguez-Diaz R, Molano RD, Lipovsek S, Rupnik M, Dicker A, Ilegems E, Zahr-Akrawi E, Molina J, Lopez-Cabeza M, Villate S,Abdulreda MH, Ricordi C, Caicedo A, Pileggi A, Berggren PO. Donor islet endothelial cells in pancreatic islet revascularization. Diabetes. 2011 Oct;60(10):2571-7.

Speier S. Experimental approaches for high-resolution in vivo imaging of islet of Langerhans biology. Curr Diab Rep. 2011 Oct;11(5):420-5.

Solimena M, Speier S. Insulin release: shedding light on a complex matter. Cell Metab. 2010 Jul 7;12(1):5-6.

Cabrera O, Jacques-Silva MC, Speier S, Yang SN, Köhler M, Fachado A, Vieira E, Zierath JR, Kibbey R, Berman DM, Kenyon NS, Ricordi C, Caicedo A, Berggren PO. Glutamate is a positive autocrine signal for glucagon release. Cell Metab. 2008 Jun;7(6):545-54.

Speier S, Nyqvist D, Cabrera O, Yu J, Molano RD, Pileggi A, Moede T, Köhler M, Wilbertz J, Leibiger B, Ricordi C, Leibiger IB, Caicedo A, Berggren PO. Noninvasive in vivo imaging of pancreatic islet cell biology. Nat Med. 2008 May;14(5):574-8.

Speier S, Nyqvist D, Köhler M, Caicedo A, Leibiger IB, Berggren PO. Noninvasive high-resolution in vivo imaging of cell biology in the anteriorchamber of the mouse eye. Nat Protoc. 2008;3(8):1278-86.

Speier S, Gjinovci A, Charollais A, Meda P, Rupnik M. Cx36-mediated couplingreduces beta-cell heterogeneity, confines the stimulating glucose concentration range, and affects insulin release kinetics. Diabetes. 2007 Apr;56(4):1078-86.

Speier S, Yang SB, Sroka K, Rose T, Rupnik M. KATP-channels in beta-cells in tissue slices are directly modulated by millimolar ATP. Mol Cell Endocrinol. 2005 Jan 31;230(1-2):51-8.

Speier S, Rupnik M. A novel approach to in situ characterization of pancreatic beta-cells. Pflugers Arch. 2003 Aug;446(5):553-8.