ZML-Seminar mit Aleksi Sutinen: "Versatile approaches to studying macromolecular dynamics using time-resolved SAXS" 27. Oktober 2026 | 15:00 Uhr | CRTD-Auditorium links

Aleksi Sutinen: Time-resolved SAXS

Am 27. Oktober 2026 begrüßt das ZML Aleksi Sutinen, Postdoctoral Fellow am EMBL Hamburg in der Arbeitsgruppe von Clément Blanchet, zu einem Gastvortrag. Der Vortrag mit dem Titel „Versatile approaches to studying macromolecular dynamics using time-resolved SAXS“ findet um 15:00 Uhr im CRTD Auditorium Links statt und wird in englischer Sprache gehalten.

Aleksi Sutinen arbeitet unter anderem mit BioSAXS, Soft-Matter-SAXS und struktureller Biologie. In seinem Vortrag gibt er Einblicke in die Möglichkeiten der zeitaufgelösten Kleinwinkel-Röntgenstreuung (Time-resolved Small-Angle X-ray Scattering, TR-SAXS) zur Untersuchung dynamischer Prozesse in biologischen Makromolekülen.

Abstract

Small-angle X-ray scattering (SAXS) is a versatile solution-based method for studying the structure, interactions, and assembly of biological macromolecules. At the EMBL P12 BioSAXS beamline at PETRA III, highly automated sample handling and data collection enable rapid characterization of a broad range of biological systems and experimental conditions, providing an efficient route from initial screening to more advanced SAXS experiments, or setting the stage for other techniques in structural biology. Beyond equilibrium measurements, SAXS can directly follow structural changes in solution as they occur. Time-resolved SAXS (TR-SAXS) at P12 extends BioSAXS towards the millisecond-to-second regime, enabling observation of processes such as conformational transitions, biomolecular interactions, folding, assembly and disassembly. Static SAXS characterization provides the essential basis for defining appropriate sample conditions, concentrations and timescales before moving to kinetic measurements. TR-SAXS capabilities at P12 include stopped-flow mixing, microfluidics and light-triggered experiments. Recent developments in sample environments, triggering, and complementary offline spectroscopy are discussed together with experimental examples, practical considerations, and current limitations.