Plant Genomics
Lecturer: Dr. Gerhard Menzel
Script: The script will be handed over at the beginning of the course.
Content
Plant genome analysis (Genomics) requires the use of high-throughput-technologies. The course is based on a genomic sugar beet (Beta vulgaris) library of 50304 BACs (bacterial artifical chromosome) with an average insert size of 125 kb. Using the biorobot MicroGrid II, participants will generate a high density filter of 9216 BAC clones (1.5 genome equivalents). By radioactive hybridization experiments with this high density filter, students will select BAC clones relevant for running projects of the working group. The DNA of selected BACs will be prepared by a large-scale purfication procedure and subsequently fragmented to a defined size by the HydroShear device. After subcloning of the DNA fragments in plasmid vectors, these subclones will be transfered to 96-well microtiter plates and spotted on membrane filters by the MicroGrid II.
We offer the opportunity to characterize the filters in a four week "Großpraktikum".
