Publikationen
2022
Petropoulos, M., Champeris Tsaniras, S., Nikou, S., Maxouri, S., Dionellis, V.S., Kalogeropoulou, A., Karamichali, A., Ioannidis, K., Danalatos, I.R., Obst, M., Naumann, R., Delinasios, G.J., Gorgoulis, V.G., Roukos, V., Anastassiadis, K., Halazonetis, T.D., Bravou, V., Lygerou, Z. and Taraviras, S. (2022) "Cdt1 overexpression drives colorectal carcinogenesis through origin overlicensing and DNA damage" J. Pathol., 259(1), 10 - 20; doi: 10.1002/path.6017
Kapplusch, F., Schulze, F., Reinke, S., Russ, S., Linge, M., Kulling, F., Kriechling, F., Höhne, K., Winkler, S., Hartmann, H., Rösen-Wolff, A., Anastassiadis, K., Hedrich, C.M. and Hofmann, S.R. (2022) "RIP2-deficiency induces inflammation in response to SV40 Large T induced genotoxic stress through altered ROS homeostasis" Clin. Immunol., 238: 108998; doi: 10.1016/j.clim.2022.108998
2021
Jin, K.X., Zuo, R., Anastassiadis, K., Klungland, A., Marr, C. and Filipczyk, A. (2021) "N6-methyladenosine (m6A) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells" Proc. Natl. Acad. Sci. USA, 118(51), e2105192118
Kobayashi, Y., Tomizawa, S.I., Ono, M., Kuroha, K., Minamizawa, K., Natsume, K., Dizdarević, S., Dočkal, I., Tanaka, H., Kawagoe, T., Seki, M., Suzuki, Y., Ogonuki, N., Inoue, K., Matoba, S., Anastassiadis, K., Mizuki, N., Ogura, A., and Ohbo K. (2021) "Tsga8 is required for spermatid morphogenesis and male fertility in mice" Development, doi: 10.1242/dev.196212
Narita, T., Ito, S., Higashijima, Y., Chu, W.K., Neumann, K., Walter, J., Satpathy, S., Liebner, T., Hamilton, W.B., Maskey, E., Prus, G., Shibata, M., Iesmantavicius, V., Brickman, J.M., Anastassiadis, K., Koseki, H., and Choudhary, C. (2021) "Enhancers are activated by p300/CBP activity-dependent PIC assembly, RNAPII recruitment, and pause release" Mol. Cell, doi: 10.1016/j.molcel.2021.03.008
Goveas, N., Waskow, C., Arndt, K., Heuberger, J., Zhang, Q., Alexopoulou, D., Dahl, A., Birchmeier, W., Anastassiadis, K., Stewart, A.F. and Kranz, A (2021) "MLL1 is required for maintenance of intestinal stem cells" PLoS Genet. 17(12), e1009250
Stepanova, V., Moczulska, K.E., Vacano, G.N., Kurochkin, I., Ju, X., Riesenberg, S., Macak, D., Maricic, T., Dombrowski, L., Schörnig, M., Anastassiadis, K., Baker, O., Naumann, R., Khrameeva, E., Vanushkina, A., Stekolshchikova, E., Egorova, A., Tkachev, A., Mazzarino, R., Duval, N., Zubkov, D., Giavalisco, P., Wilkinson, T.G., Patterson, D., Khaitovich, P. and Pääbo, S. (2021) "Reduced purine biosynthesis in humans after their divergence from Neandertals" eLife, 10, e58741
2020
Ahmed, N., Kunz, L., Hoppe, P.S., Loeffler, D., Etzrodt, M., Ortega, G.C., Hilsenbeck, O., Anastassiadis, K. and Schroeder, T. (2020) "A novel GATA2 protein reporter mouse reveals hematopoietic progenitor cell types" Stem Cell Reports; 15(2), 326 - 339; doi: 10.1016/j.stemcr.2020.06.008
Kranz, A. and Anastassiadis, K. (2020), "The role of SETD1A and SETD1B in development and disease" Biochim. Biophys. Acta Gene Regul. Mech., 1863(8), 194578; doi: 10.1016/j.bbagrm.2020.19457
Ashokkumar, D., Zhang, Q., Much, C., Bledau, A.S., Naumann, R., Alexopoulou, D., Dahl, A., Goveas, N., Fu, J., Anastassiadis, K., Stewart, A.F. and Kranz, A. (2020) "MLL4 is required after implantation whereas MLL3 becomes essential during late gestation" Development, 147(12), dev186999; doi: 10.1242/dev.186999
2019
Mameishvili, E., Serafimidis, I., Iwaszkiewicz, S., Lesche, M., Reinhardt, S., Bölicke, N., Büttner, M., Stellas, D., Papadimitropoulou, A., Szabolcs, M., Anastassiadis, K., Dahl, A., Theis, F., Efstratiadis, A. and Gavalas, A. (2019) "Aldh1b1 expression defines progenitor cells in the adult pancreas and is required for Kras-induced pancreatic cancer" Proc. Natl. Acad. Sci. USA, 116(41), 20679 - 20688, doi: 10.1073/pnas.1901075116
Mende, N., Jolly, A., Percin, G.I., Günther, M., Rostovskaya, M., Krishnan, S.M., Oostendorp, R.A.J., Dahl, A., Anastassiadis, K., Höfer, T. and Waskow, C. (2019) "Prospective isolation of non-hematopoietic cells of the niche and their differential molecular interactions with HSCs" Blood, 134(15), 1214-1226; doi: 10.1182/blood.2019000176
Schreiber, M., Weigelt, M., Karasinsky, A., Anastassiadis, K., Schallenberg, S., Petzold, C., Bonifacio, E., Kretschmer, K. and Hommel, A. (2019) "Inducible IL-7 hyperexpression influences lymphocyte homeostasis and function and increases allograft rejection" Front. Immunol. 10, 742; doi: 10.3389/fimmu.2019.00742
Chen, Y., Jones, K.L., Anastassiadis, K., Kranz, A., Stewart, A.F., Grembecka, J., Meyerson, M. and Ernst, P. (2019) "Distinct pathways affected by Menin versus MLL1/MLL2 in MLL-rearranged Acute Myeloid Leukemia" Exp. Hematol. 69, 37 - 42; doi: 10.1016/j.exphem.2018.10.001
Kavouras, M., Malandrakis, E. .E., Danis, T., Blom, E., Anastassiadis, K., Panagiotaki, P. and Exadaktylos, A. (2019) "Hox genes polymorphism depicts developmental disruption of common sole eggs." Open Life Sciences 14 (1), 549-563.
Kavouras, M., Malandrakis, E. E., Golomazou, E., Konstantinidis, I., Blom, E., Palstra, A. P., Anastassiadis, K., Panagiotaki, P. and Exadactylos, A. (2019) "Hox gene expression profiles during embryonic development of common sole." Animal Biology 69 (2): 183 - 198
2018
Tomizawa, S.I., Kobayashi, Y., Shirakawa, T., Watanabe, K., Mizoguchi, K., Hoshi, I., Nakajima, K., Nakabayashi, J., Singh, S., Dahl, A., Alexopoulou, D., Seki, M., Suzuki, Y., Royo, H., Peters, A.H.F.M., Anastassiadis, K., Stewart, A.F. and Ohbo, K. (2018) "Kmt2b conveys monovalent and bivalent H3K4me3 in mouse spermatogonial stem cells at germline and embryonic promoters" Development, 145(23), pii: dev169102
Karimova, M., Baker, O., Camgoz, A., Naumann, R., Buchholz, F. and Anastassiadis, K. (2018) "A single reporter mouse line for Vika, Flp, Dre and Cre-recombination" Sci. Rep. 8(1): 14453
Rostovskaya, M., Donsante, S., Sacchetti, B., Alexopoulou, D., Klemroth, S., Dahl, A., Riminucci, M., Bianco, P. and Anastassiadis, K. (2018) "Clonal Analysis delineates transcriptional Programs of osteogenic and adipogenic Lineages of adult Mouse skeletal Progenitors" Stem Cell Reports, 11(1), 212 - 227
Schmidt, K., Zhang, Q., Tasdogan, A., Petzold, A., Dahl, A., Arneth, B.M., Slany, R., Fehling, H.J., Kranz, A., Stewart, A.F. and Anastassiadis, K. (2018) "The H3K4 methyltransferase Setd1b is essential for hematopoietic stem cell and progenitor cell homeostasis in mice" ELife, 7, pii: e27157; doi: 10.7554/eLife.27157
Hastreiter, S., Skylaki, S., Loeffler, D., Reimann, A., Hilsenbeck, O., Hoppe, P.S., Coutu, D.L., Kokkaliaris, K.D., Schwarzfischer, M., Anastassiadis, K., Theis, F.J. and Schroeder, T. (2018) "Inductive and Selective Effects of GSK3 and MEK Inhibition on Nanog Heterogeneity in Embryonic Stem Cells" Stem Cell Reports, 11(1), 58 - 69
Allison, T.F., Smith, A.J.H., Anastassiadis, K., Sloane-Stanley, J., Biga, V., Stavish, D., Hackland, J., Sabri, S., Langerman, J., Jones, M., Plath, K., Coca, D., Barbaric, I., Gokhale, P. and Andrews, P.W. (2018) "Identification and Single-Cell Functional Charactrization of an Endodermally biased Pluripotent Substrate in Human Embryonic Stem Cells" Stem Cell Reports, 10(6), 1895 - 1907
Thieme, S., Holzbaur, A., Wiedemuth, R., Binner, A., Navratiel, K., Anastassiadis, K., Brenner, S. and Richter, C. (2018) "The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile" PLoS One 13(2): e0192437
Frith T. J., Granata, I., Wind, M., Stout, E., Thompson, O., Neumann, K., Stavish, D., Heath, P. R., Ortmann, D., Hackland, J. O., Anastassiadis, K., Gouti, M., Briscoe, J., Wilson, V., Johnson, S. L., Placzek, M., Guarracino, M.R., Andrews, P. W. and Tsakiridis, A. (2018) "Human axial progenitors generate trunk neural crest cells in vitro." Elife 7. pii: e35786.
2017
Chakraborty, D., Paszkowski-Rogacz, M., Berger, N., Ding, L., Mircetic, J., Fu, J., Iesmantavicius, V., Choudhary, C., Anastassiadis, K., Stewart, A.F. and Buchholz, F. (2017) "IncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1" Cell Rep. 21(11), 3012 - 3021; doi: 10.1016/j.celrep.2017.11.045
Schwach, V., Verkerk, A.O., Mol, M., Monshouwer-Kloots, J.J., Devalla, H.D., Orlova, V.V., Anastassiadis, K., Mummery, C.L., Davis, R.P. & Passier, R. (2017) "A COUP-TFII Human Embryonic Stem Cell Line to Identify and Select Atrial Cardiomyocytes" Stem Cell Reports, 9(6), 1765 - 1779
Urbanska, M., Winzi, M., Neumann, K., Abuhattum, S., Rosendahl, P., Müller, P., Taubenberger, A., Anastassiadis, K. & Guck, J. (2017) "Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage" Development 144(23), 4313 - 4321
Borensztein, M., Okamoto, I., Syx, L., Guilbaud, G., Picard, C., Ancelin, K., Galupa, R., Diabangouaya, P., Servant, N., Barillot, E., Surani, A., Saitou, M., Chen, C.J., Anastassiadis, K. & Heard, E. (2017) "Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass" Nat. Commun. 8(1): 1297
Akpınar, M., Lesche, M., Fanourgakis, G., Fu, J., Anastassiadis, K., Dahl, A. & Jessberger, R. (2017) "TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes" PLoS Genet. 13(9), e1006989
Chen, Y., Anastassiadis, K., Kranz, A., Stewart, A.F., Arndt, K., Waskow, C., Yokoyama, A., Jones, K., Neff, T., Lee, Y. and Ernst P. (2017) "MLL2, not MLL1, plays a major role in sustaining MLL-rearranged accute myeloid leukemia" Cancer Cell 31(6), 755 - 770
Brici, D., Zhang, Q., Reinhardt, S., Dahl, A., Hartmann, H., Schmidt, K., Goveas, N., Huang, J., Gahurova, L., Kelsey, G., Anastassiadis, K., Stewart, A.F. and Kranz, A. (2017) "Set1b, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program" Development, 144(14), 2606 - 2617
Baker, O., Tsurkan, S., Fu, J., Klink, B., Rump, A., Obst, M., Kranz, A., Schröck, E., Anastassiadis, K. and Stewart, A.F. (2017) "The contribution of homology arms to nuclease-assisted genome engineering" Nucl. Acids Res., 45(13), 8105 - 8115; doi: 10.1093/nar/gkx497
2016
Baker, O., Gupta, A., Obst, M., Zhang, Y., Anastassiadis, K., Fu, J. and Stewart, A.F. (2016) "RAC-tagging: Recombineering and Cas9-assisted targeting for protein tagging and conditional analyses" Sci. Rep., 6: 25529; doi:10.1038/srep25529
Völkner, M., Zschätzsch, M., Rostovskaya, M., Overall, R.W., Busskamp, V. Anastassiadis, K. and Karl M.O. (2016) "Retinal organoids from pluripotent stem cells efficiently recapitulate retinogenesis" Stem Cell Reports, 6(4), 525 - 538
Schmidt, M., Gutknecht, D., Anastassiadis, K., Eckes, B., Anderegg, U. and Saalbach, A. (2016) "Thy1/β3 integrin interaction-induced apoptosis of dermal fibroblasts is mediated by up-regulation of FasL" J. Invest. Dermatol., 136(2), 526 - 529
Laugsch, M., Rostovskaya, M., Velychko, S., Richter, C., Zimmer, A., Klink, B., Schrock, E., Haase, M., Neumann, K., Thieme, S., Roesler, J., Brenner, S. and Anastassiadis, K. (2016) "Functional restoration of gp91phox-oxidase activity by BAC transgenesis and gene targeting in X-linked chronic granulomatous disease iPSCs." Mol. Ther. 24(4): 812 - 822
2015
Filipczyk, A., Marr, C., Hastreiter, S., Feigelmann, J., Schwarzfischer, M., Hoppe, P.S., Loeffler, D., Kokkaliaris, K.D., Endele, M., Schauberger, B., Hilsenbeck, O., Skylaki, S., Hasenauer, J., Anastassiadis, K., Theis, F.J. and Schroeder T. (2015) "Network plasticity of pluripotency transcription factors in embryonic stem cells" Nature Cell Biology 17(10): 1235 - 1246
Wong, F. K., Fei, J. F., Mora-Bermudez, F., Taverna, E., Haffner, C., Fu, J., Anastassiadis, K., Stewart, A. F. and Huttner, W. B. (2015). "Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex." PLoS Biol. 13: e1002217
2014
Hartung, E., Becker, M., Bachem, A., Reeg, N., Jakel, A., Hutloff, A., Weber, H., Weise, C., Giesecke, C., Henn, V., Gurka, S., Anastassiadis, K., Mages, H. W. and Kroczek, R. A. (2014). "Induction of Potent CD8 T Cell Cytotoxicity by Specific Targeting of Antigen to Cross-Presenting Dendritic Cells In Vivo via Murine or Human XCR1." J. Immunol. 194(3): 1069 - 1079
Fischedick, G., Wu, G., Adachi, K., Arauzo-Bravo, M. J., Greber, B., Radstaak, M., Kohler, G., Tapia, N., Iacone, R., Anastassiadis, K., Schöler, H. R. and Zaehres, H. (2014). "Nanog induces hyperplasia without initiating tumors." Stem Cell Res. 13: 300-315.
Wobus, M., List, C., Dittrich, T., Dhawan, A., Duryagina, R., Arabanian, L. S., Kast, K., Wimberger, P., Stiehler, M., Hofbauer, L. C., Jakob, F., Ehninger, G., Anastassiadis, K. and Bornhauser, M. (2014). "Breast carcinoma cells modulate the chemoattractive activity of human bone marrow-derived mesenchymal stromal cells by interfering with CXCL12." Int. J. Cancer. 136(1): 44 - 54
Duryagina, R., Anastassiadis, K., Maitz, M., Gramm, S., Schneider, S., Wobus, M., Thieme, S., Brenner, S., Werner, C. and Bornhauser, M. (2014). "Cellular reporter systems for high-throughput-screening of interactions between bioactive matrices and human mesenchymal stromal cells." Tissue Eng. Part C Methods. 20(10): 828 - 837
Bledau, A. S., Schmidt, K., Neumann, K., Hill, U., Ciotta, G., Gupta, A., Torres, D. C., Fu, J., Kranz, A., Stewart, A. F. and Anastassiadis, K. (2014). "The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation." Development 141: 1022-35.
Denissov, S., Hofemeister, H., Marks, H., Kranz, A., Ciotta, G., Singh, S., Anastassiadis, K., Stunnenberg, H. G. and Stewart, A. F. (2014). "Mll2 is required for H3K4 trimethylation on bivalent promoters in embryonic stem cells, whereas Mll1 is redundant." Development 141: 526-37.
2013
Ivanova, A., Kalaidzidis, Y., Dirkx, R., Sarov, M., Gerlach, M., Schroth-Diez, B., Muller, A., Liu, Y., Andree, C., Mulligan, B., Munster, C., Kurth, T., Bickle, M., Speier, S., Anastassiadis, K. and Solimena, M. (2013). "Age-dependent labeling and imaging of insulin secretory granules." Diabetes 62(11), 3687 - 3696.
Filipczyk, A., Gkatzis, K., Fu, J., Hoppe, P. S., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013). "Biallelic expression of nanog protein in mouse embryonic stem cells." Cell Stem Cell 13: 12-3.
Prewitz, M. C., Seib, F. P., von Bonin, M., Friedrichs, J., Stissel, A., Niehage, C., Müller, K., Anastassiadis, K., Waskow, C., Hoflack, B., Bornhauser, M. and Werner, C. (2013). "Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments." Nat. Methods 10: 788-94.
Duryagina, R., Thieme, S., Anastassiadis, K., Werner, C., Schneider, S., Wobus, M., Brenner, S. and Bornhauser, M. (2013). "Overexpression of Jagged-1 and its Intracellular Domain in Human Mesenchymal Stromal Cells Differentially Affect the Interaction with Hematopoietic Stem and Progenitor Cells." Stem Cells Dev. 22: 2736-50.
Singh, R. P., Franke, K., Kalucka, J., Mamlouk, S., Muschter, A., Gembarska, A., Grinenko, T., Willam, C., Naumann, R., Anastassiadis, K., Stewart, A. F., Bornstein, S., Chavakis, T., Breier, G., Waskow, C. and Wielockx, B. (2013). "HIF-prolyl hydroxylase 2 (PHD2) is a critical regulator of hematopoietic stem cell maintenance during steady-state and stress." Blood 121: 5158-66.
Richter, C., Thieme, S., Bandola, J., Laugsch, M., Anastassiadis, K. and Brenner, S. (2013). "Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo." PLoS One 8: e62621.
Habib, S. J., Chen, B. C., Tsai, F. C., Anastassiadis, K., Meyer, T., Betzig, E. and Nusse, R. (2013). "A localized Wnt signal orients asymmetric stem cell division in vitro." Science 339: 1445-8.
Thieme, S., Gyarfas, T., Richter, C., Ozhan, G., Fu, J., Alexopoulou, D., Muders, M. H., Michalk, I., Jakob, C., Dahl, A., Klink, B., Bandola, J., Bachmann, M., Schrock, E., Buchholz, F., Stewart, A. F., Weidinger, G., Anastassiadis, K. and Brenner, S. (2013). "The histone demethylase UTX regulates stem cell migration and hematopoiesis." Blood 121: 2462-73.
Coronado, D., Godet, M., Bourillot, P. Y., Tapponnier, Y., Bernat, A., Petit, M., Afanassieff, M., Markossian, S., Malashicheva, A., Iacone, R., Anastassiadis, K. and Savatier, P. (2013). "A short G1 phase is an intrinsic determinant of naive embryonic stem cell pluripotency." Stem Cell Res. 10: 118-31.
Anastassiadis, K., Schnütgen, F., von Melchner, H. and Stewart, A. F. (2013). "Gene Targeting and Site-Specific Recombination in Mouse ES Cells." Methods Enzymol. 533: 133 - 155.
2012
Rostovskaya, M., Naumann, R., Fu, J., Obst, M., Mueller, D., Stewart, A. F. and Anastassiadis, K. (2012). "Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes." Genesis 51: 135-41.
Rostovskaya, M. and Anastassiadis, K. (2012). "Differential expression of surface markers in mouse bone marrow mesenchymal stromal cell subpopulations with distinct lineage commitment." PLoS One 7: e51221.
Rostovskaya, M., Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon Mediated BAC Transgenesis in Human ES Cells.” Nucleic Acids Res. 40: e150.
Wojtasz, L., Cloutier, J.M., Baumann, M., Daniel, K., Varga, J., Fu, J., Anastassiadis, K., Stewart, A.F., Reményi, A., Turner, J.M., Tóth, A. (2012): Meiotic DNA double-strand breaks and chromosome asynapsis in mice are monitored by distinct HORMAD2-independent and -dependent mechanisms. Genes Dev. 26(9): 958-73
Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling MC, Bottomley J, Brown SD, Bürger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, Friedel RH, Grosveld FG, Hansen J, Hérault Y, Hicks G, Hörlein A, Houghton R, Hrabé de Angelis M, Huylebroeck D, Iyer V, de Jong PJ, Kadin JA, Kaloff C, Kennedy K, Koutsourakis M, Kent Lloyd KC, Marschall S, Mason J, McKerlie C, McLeod MP, von Melchner H, Moore M, Mujica AO, Nagy A, Nefedov M, Nutter LM, Pavlovic G, Peterson JL, Pollock J, Ramirez-Solis R, Rancourt DE, Raspa M, Remacle JE, Ringwald M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Schick JZ, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Simpson EM, Skarnes WC, Smedley D, Stanford WL, Stewart, A. F., Stone K, Swan K, Tadepally H, Teboul L, Tocchini-Valentini GP, Valenzuela D, West AP, Yamamura KI, Yoshinaga Y, Wurst W. (2012) The mammalian gene function resource: the international knockout mouse consortium. Mamm. Genome, 23 (9-10), SI: Mouse Genomic Programs and Resources, 580 - 586
2011
Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Chenna, R., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting." Nucleic Acids Res. 39: e137.
Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nat Cell Biol.,13: 599-610.
Suckale, J., Wendling, O., Masjkur, J., Jager, M., Munster, C., Anastassiadis, K., Stewart, A. F. and Solimena, M. (2011). "PTBP1 Is Required for Embryonic Development before Gastrulation." PLoS One 6: e16992.
Hofemeister, H., Ciotta, G., Fu, J., Seibert, P. M., Schulz, A., Maresca, M., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2011). "Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis." Methods, 53 (4), 437 - 452.
Ciotta, G., Hofemeister, H., Maresca, M., Fu, J., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2011). "Recombineering BAC transgenes for protein tagging." Methods, 53 (2), 113 - 116.
2010
Liu, Y., Suckale, J., Masjkur, J., Magro, M. G., Steffen, A., Anastassiadis, K. and Solimena, M. (2010). "Tamoxifen-Independent Recombination in the RIP-CreER Mouse." PLoS One 5: e13533.
Knopf, F., Schnabel, K., Haase, C., Pfeifer, K., Anastassiadis, K. and Weidinger, G. (2010). "Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish." Proc. Natl. Acad. Sci. USA 107: 19933-8.
Andreu-Vieyra, C. V., Chen, R., Agno, J. E., Glaser, S., Anastassiadis, K., Stewart, A. F. and Matzuk, M. M. (2010). "MLL2 Is Required in Oocytes for Bulk Histone 3 Lysine 4 Trimethylation and Transcriptional Silencing." PLoS Biol. 8: e1000453.
Kranz, A., Fu, J., Duerschke, K., Weidlich, S., Naumann, R., Stewart, A. F. and Anastassiadis, K. (2010). "An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase." Genesis 48: 512-20.
Anastassiadis, K., Rostovskaya, M., Lubitz, S., Weidlich, S. and Stewart, A. F. (2010). "Precise conditional immortalization of mouse cells using tetracycline-regulated SV40 large T-antigen." Genesis 48: 220-32.
Schulz, H., Kolde, R., Adler, P., Aksoy, I., Anastassiadis, K., Bader, M., Billon, N., Boeuf, H., Bourillot, P. Y., Buchholz, F., Dani, C., Doss, M. X., Forrester, L., Gitton, M., Henrique, D., Hescheler, J., Himmelbauer, H., Hubner, N., Karantzali, E., Kretsovali, A., Lubitz, S., Pradier, L., Rai, M., Reimand, J., Rolletschek, A., Sachinidis, A., Savatier, P., Stewart, F., Storm, M. P., Trouillas, M., Vilo, J., Welham, M. J., Winkler, J., Wobus, A. M. and Hatzopoulos, A. K. (2009). "The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation." PLoS One 4: e6804.
Anastassiadis, K., Glaser, S., Kranz, A., Bernhardt, K. and Stewart, A. F. (2010). "A Practical Summary of Site-Specific Recombination, Conditional Mutagenesis, and Tamoxifen Induction of CreERT2." Methods Enzymol., 477, 109-123.
Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering Pipeline to Make Conditional Targeting Constructs." Methods Enzymol., 477, 125-144.
2009
Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F. and Stewart, A. F. (2009). "Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice." Dis. Model Mech. 2: 508-15.
Cambridge, S. B., Geissler, D., Calegari, F., Anastassiadis, K., Hasan, M. T., Stewart, A. F., Huttner, W. B., Hagen, V. and Bonhoeffer, T. (2009). "Doxycycline-dependent photoactivated gene expression in eukaryotic systems." Nat. Methods 6: 527-31.
Glaser, S., Lubitz, S., Loveland, K. L., Ohbo, K., Robb, L., Schwenk, F., Seibler, J., Roellig, D., Kranz, A., Anastassiadis, K. and Stewart, A. F. (2009). "The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis." Epigenetics Chromatin 2: 5.
Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M. M., Heninger, A. K., de Vries, I., Kittler, R., Junqueira, M., Shevchenko, A., Schulz, H., Hubner, N., Doss, M. X., Sachinidis, A., Hescheler, J., Iacone, R., Anastassiadis, K., Stewart, A. F., Pisabarro, M. T., Caldarelli, A., Poser, I., Theis, M. and Buchholz, F. (2009). "A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity." Cell Stem Cell 4: 403-15.
2008
Vintersten, K., Testa, G., Naumann, R., Anastassiadis, K. and Stewart, A. F. (2008). "Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes." Methods Mol. Biol., 415: 83-100.
2007
Lubitz, S., Glaser, S., Schaft, J., Stewart, A. F. and Anastassiadis, K. (2007). "Increased apoptosis and skewed differentiation in mouse embryonic stem cells lacking the histone methyltransferase Mll2." Mol. Biol. Cell 18: 2356-66.
Blaschke, R. J., Hahurij, N. D., Kuijper, S., Just, S., Wisse, L. J., Deissler, K., Maxelon, T., Anastassiadis, K., Spitzer, J., Hardt, S. E., Schöler, H., Feitsma, H., Rottbauer, W., Blum, M., Meijlink, F., Rappold, G. and Gittenberger-de Groot, A. C. (2007). "Targeted mutation reveals essential functions of the homeodomain transcription factor Shox2 in sinoatrial and pacemaking development." Circulation 115: 1830-8.
2006
Glaser, S., Schaft, J., Lubitz, S., Vintersten, K., van der Hoeven, F., Tufteland, K. R., Aasland, R., Anastassiadis, K., Ang, S. L. and Stewart, A. F. (2006). "Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development." Development 133: 1423-32.
Schnütgen, F., Stewart, A. F., von Melchner, H. and Anastassiadis, K. (2006). "Engineering embryonic stem cells with recombinase systems." Methods Enzymol., 420, 100 - 136.
2005
Glaser, S., Anastassiadis, K. and Stewart, A. F. (2005): "Current issues in mouse genome engineering." Nat Genet. 37 (11): 1187-93. Review
2004
Testa, G., Schaft, J., van der Hoeven, F., Glaser, S., Anastassiadis, K., Zhang, Y., Hermann, T., Stremmel, W. and Stewart, A. F. (2004). "A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles." Genesis 38: 151-8.
2002
Anastassiadis, K., Kim, J., Daigle, N., Sprengel, R., Schöler, H. R. and Stewart, A. F. (2002). "A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture." Gene 298: 159-72.
2000
Kirchhof, N., Carnwath, J. W., Lemme, E., Anastassiadis, K., Schöler, H. and Niemann, H. (2000). "Expression pattern of Oct-4 in preimplantation embryos of different species." Biol. Reprod. 63: 1698-705.
Voss, A. K., Thomas, T., Petrou, P., Anastassiadis, K., Schöler, H. and Gruss, P. (2000). "Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos." Development 127: 5449-61.
1999
Pesce, M., Anastassiadis, K. and Schöler, H. R. (1999): "Oct-4: Lessons of totipotency from embryonic stem cells." Cells Tissues Organs 165 (3-4): 144-152
1998
Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Schöler, H. and Smith, A. (1998). "Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4." Cell 95: 379-91.
Botquin, V., Hess, H., Fuhrmann, G., Anastassiadis, C., Gross, M. K., Vriend, G. and Scholer, H. R. (1998). "New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2." Genes Dev. 12: 2073-90.
De Felice, M., Ovitt, C., Biffali, E., Rodriguez-Mallon, A., Arra, C., Anastassiadis, K., Macchia, P. E., Mattei, M. G., Mariano, A., Schöler, H., Macchia, V. and Di Lauro, R. (1998). "A mouse model for hereditary thyroid dysgenesis and cleft palate." Nat. Genet. 19: 395-8.
1997
Anastassiadis, C., Rottmann, O., Hörmansdorfer, S. and Pirchner, F. (1997). "Expression of the hepatitis B surface antigen (HBsAg) under the control of mMT-1 promoter in transgenic mice can be induced by zinc sulphate, dexamethasone and lipopolysaccharide." J. Anim. Breed. Genet. 114: 225-230.
1996
Fuchs, H., Anastassiadis, C., Schlee, P., Stein, H. and Rottmann, O. (1996). "Detection of a G/T base substitution in two bleak (Alburnus alburnus) populations by RAPD." J. Appl. Ichtyol. 12: 95-97.
Kühn, R., Anastassiadis, C. and Pirchner, F. (1996). "Transfer of bovine microsatellites to the cervine (Cervus elaphus)." Anim. Genet. 27: 199-201.
Anastassiadis, C., Leyhe, B., Olsaker, I., Friedl, R., Rottmann, O., Hiendleder, S. and Erhardt, G. (1996). "Three polymorphic microsatellites for bovine chromosomes 7, 12 and 19." Anim. Genet. 27: 125-6.
1995
Anastassiadis, K., Rottmann, O., Weniger, J. H. and Pirchner, F. (1995). "Integration, Transmission und Expression von Transgenen in Mäuselinien." J. Anim. Breed. Genet. 112: 213-223.
1994
Anastassiadis, K. and Rottmann, O. (1994). "Embryotransfer bei der Maus - Einfluß von Genotyp und anderen Faktoren" Archiv für Tierzucht 37: 461-469.