Tools and Methods
C. elegans Codon Adapter:
This web tool is useful for controlling gene expression levels
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Citation:
Redemann, S., Schloissnig, S., Ernst, S., Pozniakowsky, A., Ayloo, S., Hyman, A.A., and Bringmann, H.* (2011). Codon adaptation-based control of protein expression in C. elegans. Nature Methods 8, 250-252.
https://www.nature.com/articles/nmeth.1565
https://pure.mpg.de/rest/items/item_886551_5/component/file_1388643/content
Agarose Hydrogel Microcompartments:
This method is useful for imaging larvae and adult worms over long periods of time with high optical resolution
Picture: Marina Sinner
Bringmann, H.* (2011). Agarose hydrogel microcompartments for imaging sleep- and wake-like behavior and nervous system development in Caenorhabditis elegans larvae. J Neurosci Methods 201, 78-88.
https://doi.org/10.1016/j.jneumeth.2011.07.013
Turek, M., Besseling, J., and Bringmann, H.* (2015). Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans. Journal of visualized experiments : JoVE, e52742.
https://www.jove.com/de/t/52742/agarose-microchambers-for-long-term-calcium-imaging-caenorhabditis
Non-Mendelian inheritance of entire genomes in C. elegans:
This tool is useful for generating offspring that inherit entire genomes from their grantparents
Citation:
Besseling, J., and Bringmann, H.* (2016). Engineered non-Mendelian inheritance of entire parental genomes in C. elegans. Nature Biotechnology 34, 982-986.
https://www.nature.com/articles/nbt.3643.pdf
See also:
https://doi.org/10.1016/j.devcel.2019.01.020
The OptoGenBox – a device for long-term optogenetics in C. elegans
This device is useful for applying long-term optogenetic stimulation protocols
Citation:
Busack, I., Jordan, F., Sapir, P., and Bringmann, H.* (2020). The OptoGenBox - a device for long-term optogenetics in C. elegans. J Neurogenet, 1-9.