Equipment

Analysis Flow Cytometers

LSR Fortessa (BD)

• measurement of 20 parameters: 18 fluorescence detectors, FSC and SSC
• 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
• fully automated and rapid sample acquisition (HTS, only at 3rd floor)
• LSR Fortessa Configuration

• CMCB FCCF E-Mail

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• CRTD 1^st floor south, room 1.229
• 0351-458-82138
• CRTD 3^rd floor south, room 3.113
• 0351-458-82368

LSR II (BD) 

• measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC 
• 3 lasers: Violet (405nm), Blue (488nm), Red (640nm)
• optional: fully automated and rapid sample acquisition (HTS)
• LSRII Configuration
• CRTD 1^st floor north, room 1.229
• 0351-458-82138
• CMCB FCCF E-Mail

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FACSCanto II SORP (BD)

• measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
• 3 lasers: Blue (488nm), Yellow-Green (561nm), Red (635nm)
• optional: fully automated and rapid sample acquisition (HTS)
• Canto II SORP Configuration
• CRTD 3^rd floor south, room 3.112
• 0351-458-82368
• CMCB FCCF E-Mail

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MACSQuant 10 (Miltenyi)

• measurement of 9 parameters: 7 fluorescence detectors, FSC and SSC
• 3 lasers: Violet (405nm), Blue (488nm), Red (635nm)
• automated sample acquisition (tube rack or well plates)
• integrated magnetic pre-enrichment option
• MACSQuant 10 Configuration
• CRTD 1^st floor north, in front of room 1.229 
• 0351-458-82138
• CMCB FCCF E-Mail

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MACSQuant VYB (Miltenyi)

• measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
• 3 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm)
• automated sample acquisition (tube rack or well plates)
• integrated magnetic pre-enrichment option
• MACSQuant VYB Configuration
• CRTD 3^rd floor south, room 3.112 
• 0351-458-82368
• CMCB FCCF E-Mail

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Spectral Flow Cytometer

Aurora (Cytek)

• measurement of 67 parameters: 64 fluorescence detectors, FSC and blue SSC and violet SSC
• 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
• Aurora Configuration
• CRTD 3^rd floor south, room 3.113
• 0351-458-82368
• CMCB FCCF E-Mail

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Cell Sorters

FACSMelody (BD)

• measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
• 3 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm)
• sorting into different tubes, well plates
• Melody Configuration
• CRTD 3^rd floor south, room 3.113
• 0351-458-82368
• CMCB FCCF E-Mail

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FACSAria II SORP (BD)

• measurement of 16 parameters: 14 fluorescence detectors, FSC and SSC
• 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (633nm)
• sorting into different tubes, well plates and onto microscope slides
• Aria II Configuration
• CRTD 1^st floor north, room 1.229 
• 0351-458-82138
• CMCB FCCF E-Mail

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FACSAria III (BD)

• measurement of 18 parameters: 16 fluorescence detectors, FSC and SSC
• 4 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (633nm)
• sorting into different tubes, well plates and onto microscope slides
• Aria III Configuration
• CRTD 3^rd floor south, room 3.113 
• 0351-458-82368
• CMCB FCCF E-Mail

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FACSAria Fusion (BD)

• fully enclosed in a (Class II Type A2) Biosafety Cabinet
• measurement of 20 parameters: 18 fluorescence detectors, FSC and SSC
• 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
• sorting into different tubes, well plates and onto microscope slides
• Aria Fusion Configuration
• CRTD 1^st floor north, room 1.223
• 0351-458-82124
• CMCB FCCF E-Mail

Imaging Flow Cytometry

ImageStreamX Mark II (Amnis/Millipore)

• the Imaging Flow Cytometer combines high throughput, quantitative and sensitive single cell analysis of a Flow Cytometer with microscopic imaging and spatial resolution
• 6 lasers: Violet (405nm), Blue (488nm), YellowGreen (561nm), Orange (592nm), Red (642nm), farRed (785nm)
• measurement of 12 parameters: 10 fluorescence detectors, Brightfield and SSC
• ISX Configuration
• CRTD 3^rd floor south, room 3.112 
• 0351-458-82368
• CMCB FCCF E-Mail

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Spectral Imaging Cell Sorter

FACSDiscover S8 (BD)

• fully enclosed in a (Class II Type A2) Biosafety Cabinet
• 76 fluorescence detectors, 6 imaging detectors, FSC and SSC
• 5 lasers: UV (349nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (637nm)
• sorting into different tubes, well plates and onto microscope slides
• Discover S8 Configuration
• CRTD 3^rd floor south, room 3.113
• 0351-458-82369
• CMCB FCCF E-Mail

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MACS Technology

autoMACS Pro (Miltenyi)

• MACS Microbead Technology for magnetic cell sorting
• walk-away cell isolation with automated labeling and separation
• fast and gentle isolation of virtually any cell type from any species
• CRTD 1^st floor north, in front of room 1.229 (1.227) 
• 0351-458-82138
• CMCB FCCF E-Mail

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Analysis Computers

Computers on 3^rd floor

• FACSDiva^TM Software, Becton, Dickinson and Company (BD)
• FlowJo^TM Software (for Windows) Version 10 - FlowJo, LLC & Becton, Dickinson and Company (BD)
• SpectroFlo^(R) Software (Cytek)
• IDEAS^® Software - Amnis & Luminex

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