Equipment
Analysis Flow Cytometers
LSR Fortessa (BD)
![BD LSR Fortessa 1st floor](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-LSR-F-1st-Anne-Gompf_20210107_195149-2.jpg/@@images/d656501b-2da9-4f95-b679-4ea6a625df53.jpeg)
BD LSR Fortessa 1st floor at the CMCB FCCF
- measurement of 20 parameters: 18 fluorescence detectors, FSC and SSC
- 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
- fully automated and rapid sample acquisition (HTS, only at 3rd floor)
- LSR Fortessa Configuration
![BD LSR Fortessa 3rd floor](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-LSR-F-3rd-Anne-Gompf_201231_155154-2.jpg/@@images/d05d849c-f666-4afe-b611-c7d0df80bc9b.jpeg)
BD LSR Fortessa 3rd floor at the CMCB FCCF
- CRTD 1st floor south, room 1.229
- 0351-458-82138
- CRTD 3rd floor south, room 3.113
- 0351-458-82368
LSR II (BD)
![BD LSR II at CRTD Flow Cytometry Facility](https://tu-dresden.de/cmcb/ressourcen/bilder/CMCBpdc/csm_BD_LSR_II__ANne_Gompf-IMG_3891_1200_d250e04d73.jpg/@@images/9ec31b04-0fac-45ec-8e5f-a445c2a48df5.jpeg)
BD LSR II at the CMCB FCCF
- measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
- 3 lasers: Violet (405nm), Blue (488nm), Red (640nm)
- optional: fully automated and rapid sample acquisition (HTS)
- LSRII Configuration
- CRTD 1st floor north, room 1.229
- 0351-458-82138
- CMCB FCCF E-Mail
FACSCanto II SORP (BD)
![BD FACSCanto II SORP at CRTD Flow Cytometry Facility](https://tu-dresden.de/cmcb/ressourcen/bilder/CMCBpdc/csm_BD_FACSCanto_II_SORP__Anne_Gompf-IMG_3885-2_1200_11e63d1808.jpg/@@images/c89e59a5-b594-46f6-9c38-f857cdc37446.jpeg)
BD FACSCanto II SORP at the CMCB FCCF
- measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
- 3 lasers: Blue (488nm), Yellow-Green (561nm), Red (635nm)
- optional: fully automated and rapid sample acquisition (HTS)
- Canto II SORP Configuration
- CRTD 3rd floor south, room 3.112
- 0351-458-82368
- CMCB FCCF E-Mail
MACSQuant 10 (Miltenyi)
![MACSQuant at CRTD Flow Cytometry Facility](https://tu-dresden.de/cmcb/ressourcen/bilder/CMCBpdc/csm_MACSQuant__Franziska_Clauss-01_1200_d07506ea40.jpg/@@images/43d38aa6-a2b9-455b-b83a-835547d4b5c0.jpeg)
MACSQuant at the CMCB FCCF
- measurement of 9 parameters: 7 fluorescence detectors, FSC and SSC
- 3 lasers: Violet (405nm), Blue (488nm), Red (635nm)
- automated sample acquisition (tube rack or well plates)
- integrated magnetic pre-enrichment option
- MACSQuant 10 Configuration
- CRTD 1st floor north, in front of room 1.229
- 0351-458-82138
- CMCB FCCF E-Mail
MACSQuant VYB (Miltenyi)
![MACSQuant Vyb](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/MACSQuant-Vyb-Anne-Gompf_20210107_162551-2.jpg/@@images/3975c591-0ad2-4891-b299-1c1bbc47c0a3.jpeg)
MACSQuant VYB at the CMCB FCCF
- measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
- 3 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm)
- automated sample acquisition (tube rack or well plates)
- integrated magnetic pre-enrichment option
- MACSQuant VYB Configuration
- CRTD 3rd floor south, room 3.112
- 0351-458-82368
- CMCB FCCF E-Mail
Spectral Flow Cytometer
Aurora (Cytek)
![Aurora Spectral Analyzer](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/CMCB-Aurora.jpg/@@images/843aac3a-d601-470d-bccd-08e015c41e65.jpeg)
Cytek Aurora at the CMCB FCCF
- measurement of 67 parameters: 64 fluorescence detectors, FSC and blue SSC and violet SSC
- 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
- Aurora Configuration
- CRTD 3rd floor south, room 3.113
- 0351-458-82368
- CMCB FCCF E-Mail
Cell Sorters
FACSMelody (BD)
![BD FACSMelody](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-Melody-Anne-Gompf_20210107_161903-2.jpg/@@images/3ea36ebe-e929-4d68-8457-39dbc3215728.jpeg)
BD FACSMelody at the CMCB FCCF
- measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
- 3 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm)
- sorting into different tubes, well plates
- Melody Configuration
- CRTD 3rd floor south, room 3.113
- 0351-458-82368
- CMCB FCCF E-Mail
FACSAria II SORP (BD)
![BD FACSAria II](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-Aria-II-Anne-Gompf_201231_151559-2.jpg/@@images/32a8a51f-7afd-46e2-8ace-709b1d8e2490.jpeg)
BD FACSAria II
- measurement of 16 parameters: 14 fluorescence detectors, FSC and SSC
- 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (633nm)
- sorting into different tubes, well plates and onto microscope slides
- Aria II Configuration
- CRTD 1st floor north, room 1.229
- 0351-458-82138
- CMCB FCCF E-Mail
FACSAria III (BD)
![Sorter BD FACSAria III](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-FACSAria-III-Anne-Gompf-IMG_3881.jpg/@@images/c17f104c-5ca3-4675-afca-75dc5cdfdfa6.jpeg)
BD FACSAria III at the CMCB FCCF
- measurement of 18 parameters: 16 fluorescence detectors, FSC and SSC
- 4 lasers: Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (633nm)
- sorting into different tubes, well plates and onto microscope slides
- Aria III Configuration
- CRTD 3rd floor south, room 3.113
- 0351-458-82368
- CMCB FCCF E-Mail
FACSAria Fusion (BD)
![BD FACSAria Fusion](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/BD-Aria-Fusion-Anne-Gompf_201231_152543-2.jpg/@@images/7fb1dba5-2488-40cd-a000-915261edaef5.jpeg)
BD FACSAria Fusion
- fully enclosed in a (Class II Type A2) Biosafety Cabinet
- measurement of 20 parameters: 18 fluorescence detectors, FSC and SSC
- 5 lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm)
- sorting into different tubes, well plates and onto microscope slides
- Aria Fusion Configuration
- CRTD 1st floor north, room 1.223
- 0351-458-82124
- CMCB FCCF E-Mail
Imaging Flow Cytometry
ImageStreamX Mark II (Amnis/Millipore)
![ImageStreamX Mark II at CRTD Flow Cytometry Facility](https://tu-dresden.de/cmcb/ressourcen/bilder/CMCBpdc/csm_ImageStream__Anne_Gompf-IMG_3973_1200_f97e226800.jpg/@@images/f069a628-f45a-427f-bd72-4582512a1568.jpeg)
ImageStreamX Mark II at the CMCB FCCF
- the Imaging Flow Cytometer combines high throughput, quantitative and sensitive single cell analysis of a Flow Cytometer with microscopic imaging and spatial resolution
- 6 lasers: Violet (405nm), Blue (488nm), YellowGreen (561nm), Orange (592nm), Red (642nm), farRed (785nm)
- measurement of 12 parameters: 10 fluorescence detectors, Brightfield and SSC
- ISX Configuration
- CRTD 3rd floor south, room 3.112
- 0351-458-82368
- CMCB FCCF E-Mail
Spectral Imaging Cell Sorter
FACSDiscover S8 (BD)
![FACSDiscover S8](https://tu-dresden.de/cmcb/technologie-plattform/facilities/flow-cytometry/ressourcen/bilder/20240402_141945-2.jpg/@@images/eff64079-b3a6-4967-a31d-c020684b5d49.jpeg)
BD FACSDiscover S8
- fully enclosed in a (Class II Type A2) Biosafety Cabinet
- 76 fluorescence detectors, 6 imaging detectors, FSC and SSC
- 5 lasers: UV (349nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (637nm)
- sorting into different tubes, well plates and onto microscope slides
- Discover S8 Configuration
- CRTD 3rd floor south, room 3.113
- 0351-458-82369
- CMCB FCCF E-Mail
MACS Technology
autoMACS Pro (Miltenyi)
![AutoMACS Pro at CRTD Flow Cytometry Facility](https://tu-dresden.de/cmcb/ressourcen/bilder/CMCBpdc/csm_AutoMACS_Pro_Sp-Ed-__Franziska_Clauss-01_1200_a6a316ba8e.jpg/@@images/e257f9d2-32a2-4088-891d-24102deb460c.jpeg)
AutoMACS Pro at CMCB FCCF
- MACS Microbead Technology for magnetic cell sorting
- walk-away cell isolation with automated labeling and separation
- fast and gentle isolation of virtually any cell type from any species
- CRTD 1st floor north, in front of room 1.229 (1.227)
- 0351-458-82138
- CMCB FCCF E-Mail
Analysis Computers
Computers on 3rd floor
- FACSDivaTM Software, Becton, Dickinson and Company (BD)
- FlowJoTM Software (for Windows) Version 10 - FlowJo, LLC & Becton, Dickinson and Company (BD)
- SpectroFlo(R) Software (Cytek)
- FlowlogicTM Software - Inivai Technologies
- IDEAS® Software - Amnis & Luminex