Equipment
The Molecular Imaging and Manipulation Facility offers a range of devices and techniques, which are mainly aimed at single-molecule studies. Currently, the following techniques are available:
| Atomic Force Microscopy (AFM) | Topography imaging, Force spectroscopy and molecular manipulation |
| Optical Tweezers | Force spectroscopy and molecular manipulation |
|
Spinning Disk Confocal Microscopy |
High-speed 3D confocal imaging |
|
Superresolution Localization Microscopy (PALM/STORM/...) |
Superresolution fluorescence imaging |
|
Total Internal Reflection Fluorescence Microscopy (TIRF) |
Structural imaging and single-molecule tracking with improved fluorescence signal-to-noise ratio and thus high localization precision |
|
Fluorescence Recovery after Photobleaching (FRAP) |
Evaluation of molecular dynamics |
|
Epi-Fluorescence, DIC, Darkfield and Brightfield |
Standard wide-field imaging |
|
Interferometric Scattering Microscopy (iSCAT) |
Label-free detection of biomolecules, High-speed single-molecule tracking |
The iSCAT setup furthermore provides you with the opportunity to work at a home-built microscopy setup which allows for customization of the measurements. We plan to provide more setups like that in the future. Feel free to contact us and provide us with wishes/ideas you might have.
If you are already a user of the MIM Facility you can make reservations in the booking calendar of the CMCB Technology Platform
Microscopes available in the MIM Facility
Combined with a fluorescence microscope.
Equipped with petri dish heater and CellHesion module for extended z-ranges (e.g. for cells).
Operation in air and liquid environment.
Contact-, Tapping-, force modulation-, and Q.I. (force mapping imaging) mode.
Force spectroscopy/molecular manipulation.
[Details] [Book this device]
Dual-trap optical tweezers with 3-color confocal laser scanning for simultaneous flurescence measurement.
Molecular Manipulation.
Force Spectroscopy.
TIRF, spinning disk, epi, phase contrast;
Superresolution Localization Microscopy (STORM/PALM/...) measurements.
Equipped with two high-end EMCCD cameras, objective heater and stage heater/cooler.
Laser lines: 405, 488, 561, 647 nm.
Halogen Lamp (w/ filters for fluorescence excitation).
[Details] [Book this device]
Imaging in wide-field (epi, dark-field, phase contrast) and TIRF.
FRAP measurements.
Equipped with high-end EMCCD camera, objective heater and stage heater/cooler.
Laser lines: 405, 488, 561, 647 nm.
LEDs: 395, 440, 470, 510, 550, 575, 640 nm.
[Details] [Book this device]
Home-built iSCAT microscope;
High-speed & high-precision tracking of single molecules
Label-free detection of single molecules.
Combination with fluorescence detection is possible.