Abgeschlossene Dissertationen
Komponenten- und Technologieentwicklung zur mikrofluidischen Abbildung einer biotechnolgischen Prozesskette
Art der Abschlussarbeit
Dissertation
Autoren
- Häfner, Sebastian
Betreuer
- Prof. Dr.-Ing. Andreas Richter
Weitere Betreuer
Prof. Brigitte Voit
Abstract
The biotechnology is one of the key enabling technologies of the 21st century. Due
to their potentials in solving the world hunger problem, finding sustainable energy
productions or cures for diseases the biotechnology has become one of the most
important research areas over the last decades. In the biotechnological research
microfluidic systems become more and more important because of their various
advantages over conventional systems. Single cell analytic as well as high-throughput
drug discovery or tissue engineering are only a few examples for microfluidics in
biotechnology. With an increase in the applications also the requirements on the
microfluidic systems and their functionalities increase. Normally, active elements
like valves are used to solve most of the microfluidic operations, but they are not
capable to address all requirements which are needed to fulfil a biotechnological
process line.
The presented work attempts to set up microfluidic systems for a biotechnological
process line by using micrometre actuators made of stimuli-responsive polymers.
Various hydrogel types were investigated regarding their behaviour in different
media. Here, it is shown that the gels’ chemistry has a strong influence on the
media compatibility.
The photolithagraphic process for actuator micropatterning is optimised and
used in a production technique to fabricate high integrated microfluidic system for
the cultivation of microorganisms.
For separation tasks of microorgansims or molecules the normally used thermal
based concept to adopt hydrogel properties is changed to a chemical concept.
This concept is successfully evaluated for a micropore and a molecular sieve based
on hydrogels. The usage of hydrogel-based molecular sieves as matrices for (bio-
)chemical reactions or as storing elements with a dispensing funtionality is discussed.
to their potentials in solving the world hunger problem, finding sustainable energy
productions or cures for diseases the biotechnology has become one of the most
important research areas over the last decades. In the biotechnological research
microfluidic systems become more and more important because of their various
advantages over conventional systems. Single cell analytic as well as high-throughput
drug discovery or tissue engineering are only a few examples for microfluidics in
biotechnology. With an increase in the applications also the requirements on the
microfluidic systems and their functionalities increase. Normally, active elements
like valves are used to solve most of the microfluidic operations, but they are not
capable to address all requirements which are needed to fulfil a biotechnological
process line.
The presented work attempts to set up microfluidic systems for a biotechnological
process line by using micrometre actuators made of stimuli-responsive polymers.
Various hydrogel types were investigated regarding their behaviour in different
media. Here, it is shown that the gels’ chemistry has a strong influence on the
media compatibility.
The photolithagraphic process for actuator micropatterning is optimised and
used in a production technique to fabricate high integrated microfluidic system for
the cultivation of microorganisms.
For separation tasks of microorgansims or molecules the normally used thermal
based concept to adopt hydrogel properties is changed to a chemical concept.
This concept is successfully evaluated for a micropore and a molecular sieve based
on hydrogels. The usage of hydrogel-based molecular sieves as matrices for (bio-
)chemical reactions or as storing elements with a dispensing funtionality is discussed.
Schlagwörter
-
Berichtsjahr
2018