Publication in Nature Communications

Researchers from the Institute of Medical Informatics and Biometry (IMB) at TU Dresden, together with colleagues from the San Raffaele-Telethon Institute for Gene Therapy in Milan (Italy), developed a method to identify typical patterns in gene therapeutic time-course data and thus better quantify clonal evolution. The broad applicability of the methodology could be demonstrated using mathematical models and specific in vitro and in vivo data sets. The results show that the presented method is suitable to overcome systematic biases in clonal quantification and thus represents an essential prerequisite for better evaluating the safety and long-term efficacy of gene therapeutic approaches.

The work of Sebastian Wagner, Christoph Baldow, Dr. Ingmar Glauche and other colleagues was published in Nature Communications on June 28, 2022. (S. Wagner et al., Clonal reconstruction from co-occurrence of vector integration sites accurately quantifies expanding clones in vivo, https://www.nature.com/articles/s41467-022-31292-6).