Publications

2016

Rother S, Samsonov S, Hempel U, Vogel S, Möller S, Blaszkiewicz J, Köhling S, Schnabelrauch M, Rademann J, Pisabarro MT, Hintze V, Scharnweber D. Sulfated hyaluronan alters the tissue homeostasis by changing the interaction profile of TIMP-3 with the endocytic receptor LRP-1 cluster II and IV. Biomacromolecules 2016; 17:3252-3261.

Schmidt J, Kliemt S, Preissler C, Möller S, von Bergen M, Hempel U, Kalkhof S. Osteoblast-released matrix vesicles - Regulation of activity and composition by sulfated and non-sulfated glycosaminoglycans. Mol Cell Proteomics 2016;15:558-572.

Vogel S, Arnoldini S, Möller S, Schnabelrauch M, Hempel U. Sulfated hyaluronan alters fibronectin matrix assembly and promotes osteogenic differentiation of human bone marrow stromal cells. Scientific Reports 2016; 6:36418, doi: 10.1038/srep36418.

Hempel U, Müller K, Preissler C, Noack C, Boxberger S, Dieter P, Bornhäuser M, Wobus M. Human bone marrow stromal cells: A reliable, challenging tool for in vitro osteogenesis and bone engineering approaches. Stem Cells Int 2016, accepted 22.04.16.

Lutter A-H, Hempel U, Anderer U, Dieter P. Biphasic influence of PGE₂ on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7 cells. Prostaglandins, Leukotrienes & Essential Fatty Acids 2016, accepted 16.03.16.

2015

Scharnweber D, Hübner L, Rother S, Hempel U, Anderegg U, Samsonow SA, Pisabarro MT, Hofbauer L, Schnabelrauch M, Franz S, Simon J, Hintze V. Glycosaminoglycan derivatives: Promising candidates for the design of functional biomaterials. J Mat Sci: Mat Med 2015;26:232.

Kokesch-Himmelreich J, Woltmann B, Torger B, Rohnke M, Arnhold S, Hempel U, Müller M, Janek J. Detection of organic nanoparticles in human bone marrow-derived stromal cells using ToF-SIMS and PCA. Analyt Bioanalyt Chem 2015;407:4555-4565.

Elschner C, Noack C, Preißler C, Krause A, Scheler U, Hempel U. In vitro response of human mesenchymal stem cells to titanium coated PEEK films and their suitability for magnetic resonance imaging. Journal of Materials Science & Technology 2015;31:427-436.

Müller M, Torger B, Vehlow D, Urban B, Woltmann B, Hempel U. Drug delivery and cell interaction of adhesive poly(ethyleneimine)/sulfated polysaccharide complex particle films. Biointerphases 2015, ds.doi.org/10.111671.4913195.

Noack C, Hempel U, Preissler C, Dieter P. Prostaglandin E2 impairs osteogenic and facilitates adipogenic differentiation of human mesenchymal stromal cells. Prostaglandins, Leukotrienes & Essential Fatty Acids 2015;94:91-98.

2014

Miron A, Rother S, Huebner L, Hempel U, Käppler I, Moeller S, Schnabelrauch M, Scharnweber D, Hintze V. Sulfated hyaluronan influences the formation of artificial extracellular matrices and the adhesion of osteogenic cells. Macromolecular Biosci 2014;14:1783-94.

Kalkhof S, Förster Y, Schmidt J, Schulz M, Baumann S, Weißflog A, Gao W, Hempel U, Eckelt U, Rammelt S, Von Bergen M. Proteomics and metabolomics for in situ monitoring of wound healing. BioMed Research International 2014; 934848.

Hempel U, Preissler C, Vogel S, Möller S, Hintze V, Becher J, Schnabelrauch M, Rauner M, Hofbauer LC, Dieter P. Artificial extracellular matrices with oversulfated glycosaminoglycan derivatives promote the differentiation of osteoblast-precursor cells and premature osteoblasts. J Biomed Res Int 2014;938368.

Hempel U, Matthäus C, Preissler C, Möller S, Hintze V, Dieter P. Artificial matrices with high-sulfated glycosaminoglycans and collagen are anti-inflammatory and pro-osteogenic for human mesenchymal stromal cells. J Cell Biochem 2014,115:1561-1571.

Helth A, Gostin PF, Oswald S, Wendrock H, Wolff U, Hempel U, Arnhold S, Calin M, Eckert J, Gebert A. Chemical nanoroughening of Ti40Nb surfaces and its effect on human mesenchymal stromal cell response. J Biomed Mater Res B Appl Biomat 2014;1:31-41.

Woltmann B, Torger B, Müller M, Hempel U. Interaction Between Immobilized Polyelectrolyte Complex Nanoparticles and Human Mesenchymal Stromal Cells. International Journal of Nanomedicine 2014;9:2205-2215.

Mai M, Hempel U, Hacker M, Dieter P. Effects of HyStem-HP hydrogel elasticity on osteogenic differentiation of human mesenchymal stromal cells. Cellular and Molecular Bioengineering 2014; 7:155-164.

2013

Helth A, Gostin PF, Oswald S, Wendrock H, Wolff U, Hempel U, Arnhold S, Calin M, Eckert J, Gebert A. Chemical nanoroughening of Ti40Nb surfaces and its effect on human mesenchymal stromal cell response. J Biomed Mater Res B Appl Biomater. 2013 Jul 11. doi: 10.1002/jbm.b.32976.

Goergen J, Wenisch S, Raabe O, Moritz A, Schlewitz G, Schnettler R, Hempel U, Heiss C, Arnhold S.  Characterization of bone-marrow derived stem cells in osteoporotic models of the rat. ISRN Stem Cells 2013; doi 10.1155/2013/262451.

Förster Y, Gao W, Demmrich A, Hempel U, Hofbauer LC, Rammelt S. Monitoring the first stages of bone healing with microdialysis. Acta Orthopaedica 2013;84(1):76-81.

Wolf-Brandstetter C, Roessler S, Storch S, Hempel U, Gbureck U, Nies B, Bierbaum S, Scharnweber D.  Physicochemical and cell biological characterization of PMMA bone cements modified with additives to increase bioactivity. J Biomed Mater Res Part B 2013;101(4):599-609.

Bernstein P, Sperling I, Corbeil D, Hempel U, Fickert S. Progenitor cells from cartilage – no osteoarthritis-grade-specific differences in stem cell marker expression. Biotech Progress 2013;29(1):206-212.

Hempel U, Hefti T, Dieter P, Schlottig F. Response of human bone marrow stromal cells, MG-63, and SaOS-2 to titanium-based dental implant surfaces with different topography and surface energy. Clin Oral Impl Res 2013, 24: 174-182.

Kliemt S, Lange C, Otto W, Hintze V, Möller S, von Bergen M, Hempel U, Kalkhof S. Sulfated hyaluronan containing collagen matrices enhance cell-matrix-interaction, endocytosis, and osteogenic differentiation of human mesenchymal stromal cells. J Proteome Res 2013;12:378-389.

Büttner M, Möller S, Keller M, Huster D, Schiller J, Schnabelrauch M, Dieter P, Hempel U. Over-sulfated chondroitin sulfate derivatives induce osteogenic differentiation of human mesenchymal stromal cells independent of bone morphogenetic protein-2 and transforming growth factor-b1 signalling. J Cell Physiol 2013;228:330-340.

2012

Hempel U, Möller S, Noack C, Hintze V, Scharnweber D, Schnabelrauch M, Dieter P. Sulfated hyaluronan/collagen I-matrices enhance osteogenic differentiation of human mesenchymal stromal cells in vitro even in the absence of dexamethasone. Acta Biomaterialia 2012;8:4064-4072.

Hempel U, Hintze V, Möller S, Schnabelrauch M, Scharnweber D, Dieter P. Artificial extracellular matrices composed of collagen I and sulfated hyaluronan with adsorbed transforming growth factor b1 promote collagen synthesis of human mesenchymal stromal cells. Acta Biomat 2012; 8:659-666.

Salbach J, Rachner T, Rauner M, Hempel U, Anderegg U, Franz S, Simon J, Hofbauer L. Regenerative potential of glycosaminoglycans for skin and bone. J Mol Med 2012;90:625-635.

Wolf-Brandstetter C, Hempel U, Clyens S, Gandhi AA, Korostynska O, Oswald S, Tofail SA, Theilgaard N, Wiesmann HP, Scharnweber D. Impact of heat-treatment on interactions of contact-poled biphasic calcium phosphates with proteins and cells. Acta Biomaterialia 2012;8:3468-77.

Garbe AI, Roscher A, Schüler C, Lutter AH, Glösmann M, Bernhardt R, Chopin M, Hempel U, Hofbauer LC, Rammelt S, Egerbacher M, Erben RG, Jessberger R. Regulation of bone mass and osteoclast function depend on the F-actin modulator SWAP-70. J Bone Miner Res 2012;27:2085-2096.

2011

Hamann C, Goettsch C, Mettelsiefen J, Henkenjohann V, Rauner M, Hempel U, Bernhardt R, Fratzl-Zelman N, Roschger P, Rammelt S, Günther KP, Hofbauer LD. Delayed bone regeneration and low bone mass in a rat model of insulin-resistant type 2 diabetes mellitus is due to impaired osteoblast function. Am J Physiol Endocrinol Metab 2011; 301:E1220-1228.

Fickert S, Schröter-Bobsin U, Groß AF, Hempel U, Wojciechowski C, Rentsch C, Corbeil D, Günther KP. Human mesenchymal stem cell proliferation and osteogenic differentiation during long-term ex vivo cultivation is not age dependent. J Bone Miner Metab 2011; 29:224-235.

Goettsch C, Rauner M, Hamann C, Sinningen K, Hempel U, Bornstein SR, Hofbauer LC,. Nuclear Factor of activated T cells mediates oxidised LDL-induced calcification of vascular smooth muscle cells. Diabetologia 2011, 54:2690-2701.

Goettsch C, Rauner M, Pycyna N, Hempel U, Bornstein SR, Hofbauer LC,. miR-125b regulates calcification of vascular smooth muscle cells. Am J Pathol 2011; 179:1594-1600.

2010

Kunze R, Hempel U, Dieter P. Differential effect of chondroitin-4-sulfate on the immediate and delayed prostaglandin E(2) release from osteoblasts. Prostaglandins other Lipid Mediat 2010; 92:8-12.

Preußker S, Korneli N, Klimm W, Richter G, Hempel U, Koch R. In-vitro-Mineralisation durch Pulpafibroblasten in Abhängigkeit von Wurzelwachstum und Stimulanzien. Deutsche  Zahnärztliche Zeitschrift 2010; 65: 81-90.

Hempel U, Hefti T, Kalbacova M, Wolf-Brandstetter C, Dieter P, Schlottig F. Response of osteoblast-like SAOS-2 cells to zirconia ceramics with different surface topographies. Clin Oral Impl Res 2010; 21:174-181.

Kunze R, Rösler M, Möller S, Schnabelrauch M, Riemer T, Hempel U, Dieter P. Sulfated hyaluronan derivatives reduce the proliferation rate of primary rat calvarial osteoblasts. Glycoconj J 2010; 27:151-158.

Lutter A-H, Hempel U, Wolf-Brandstetter C, Garbe A, Goettsch C, Hofbauer L, Jessberger R, Dieter P. A novel resorption assay to assess osteoclast function based on an osteoblast-derived native extracellular matrix. J Cell Biochem 2010, epub.

2009

Bernhardt A, Lode A, Mietrach C, Hempel, U, Hanke T, Gelinsky M. In vitro osteogenic potential of human bone marrow stromal cells cultivated in porous scaffolds from mineralized collagen. J Biomed Mater Res A 2009;90:852-862.

Helas S, Goettsch C, Schoppet M, Zeitz U, Hempel U, Morawietz H, Kostenuik PJ, Erben RG, Hofbauer LC. Inhibition of Receptor Activator of NF-κB Ligand by Denosumab Attenuates Vascular Calcium Deposition in Mice. Am J Pathol 2009;175:473-478.

Michael J, Schönzart L, Israel I, Beutner R, Scharnweber D, Worch H, Hempel U, Schwenzer B. Oligonucleotide-RGD peptide conjugates for surface modification of titanium implants and improvement of osteoblast adhesion. Bioconjugate Chem 2009;20:710-718.

2008

Douglas T, Heinemann S, Hempel U, Mietrach C, Knieb C, Bierbaum S, Scharnweber D, Worch H. Characterization of collagen II fibrils containing biglycan and their effect as a coating on osteoblast adhesion and proliferation. J Mater Sci Mater Med  2008;19:1653-60.

Douglas T, Hempel U, Mietrach C, Viola M, Vigetti D, Heinemann S, Bierbaum S, Scharnweber D, Worch H. Influence of collagen-fibril-based coatings containing decorin and biglycan on osteoblast behaviour. J Biomed Mater Res A 2008;84:805-816.

Kalbacova M, Kalbac M, Dunsch L, Hempel U. Influence of single-walled carbon nanotube films on metabolic activity and adherence of human osteoblasts. Carbon 2007;45:2266-2272.

Menschikowski M, Hagelgans A, Hempel U, Lattke P, Ismailov I, Siegert G. On interaction of activated protein C with human aortic smooth muscle cells attenuating the secretory group IIA phospholipase A(2) expression. Thromb Res 2008;122:69-76.

Noack B, Görgens H, Hempel U, Fanghänel J, Hoffmann T, Ziegler A, Schackert HK. Cathepsin C gene variants in aggressive periodontitis. J Dent Res 2008;87:958-963.

2007

Douglas T, Heinemann S, Mietrach C, Hempel U, Bierbaum S, Scharnweber D, Worch H. Interactions of collagen types I and II with chondroitin sulfates A-C and their effect on osteoblast adhesion. Biomacromolecules 2007;8:1085-1092.

Douglas T, Hempel U, Mietrach C, Heinemann S, Scharnweber D, Worch  H. Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: Characterisation and influence on osteoblast behaviour. Biomol Eng 2007;24:455-458.

Eckelt U, Pilling E, Mai R, Huhle M, Bierbaum S, Bernhardt R, Scharnweber D, Kuhlisch E, Hempel U, Stadlinger B. Beschleunigte Osseointegration durch Implantatbeschichtungen mit Bestandteilen der extrazellulären Matrix. Z Zahnärztl Impl 2007:101-111.

Kalbacova M, Kalbac M, Dunsch L, Kromka A, Vaněček B, Rezek B, Hempel U, Kmoch S. The effect of SWCNT and nano-diamond films on human osteoblast cells. Phys Stat Sol B 2007;244:4356–4359.

Kalbacova M, Roessler S, Hempel U, Tsaryk R, Peters K, Scharnweber D, Kirkpatrick JC, Dieter P. The effect of electrochemically simulated titanium cathodic corrosion products on ROS production and metabolic activity of osteoblasts and monocytes/macrophages. Biomaterials
2007;28:3263-72.

Michael J, Beutner R, Hempel U, Scharnweber D, Worch H, Schwenzer B. Surface modification of titanium-based alloys with bioactive molecules using electrochemically fixed nucleic acids. J Biomed Mater Res Appl Biomater 2007;80B:146-155.

Stadlinger B, Pilling E, Huhle M, Mai R, Bierbaum S, Bernhardt R, Scharnweber D, Kuhlisch E, Hempel U, Eckelt U. Influence of extracellular matrix coatings on implant stability and osseointegration: An animal study. J Biomed Mater Res Appl Biomater 2007; 83B:222 -231.

Tsaryk R, Kalbacova M, Hempel U, Scharnweber D, Unger RE, Dieter P, Kirkpatrick CJ, Peters K. Response of human endothelial cells to oxidative stress on Ti6Al4V alloy. Biomaterials 2007;28:806-813.

2006

Kalbacova M, Kalbac M, Dunsch L, Kataura H, Hempel U. The study of the interaction of human mesenchymal stem cells and monocytes/macrophages with single-walled carbon nanotube films. Physica Status solidi B 2006;243:3514-3518.

Reinstorf A, Hempel U, Olgemöller F, Domaschke H, Schneiders W, Mai R, Stadlinger B, Rösen-Wolff A, Rammelt S, Gelinsky M, Pompe W. O-Phospho-L-serine modified calcium phosphate cements – material properties, in vitro and in vivo investigations. Mat Wiss Werkstofftech 2006;37:491-503.


2000-2005

Becker D, Geißler U, Hempel U, Bierbaum S, Scharnweber D, Worch H, Wenzel K-W. Proliferation and differentiation of rat calvarial osteoblasts on collagen type I-coated titanium alloy. J Biomed Mater Res 2002;59:516-527.

Bierbaum S, Beutner R, Hanke T, Scharnweber D, Hempel U, Wenzel K-W, Worch H. Modification of Ti6Al4V surfaces using collagen I, III and fibronectin. I. Biochemical and morphological characterization of the adsorbed matrix. J Biomed Mater Res 2003;67A:421-430.

Bierbaum S, Hempel U, Geißler U, Hanke T, Scharnweber D, Wenzel K-W, Worch H. Modification of Ti6Al4V surfaces using collagen I, III and fibronectin. II. Influence on osteoblasts responses. J Biomed Mater Res 2003;67A:431-8.

Brasack I, Böttcher H, Hempel U. Biocompatibility of modified silica-protein composite layers. J Sol-Gel Sci Technol 2000;19:479-482.

Fichtner F, Koslowski R, Augstein A, Hempel U, Röhlecke C, Kasper M. Bleomycin induces IL-8 and ICAM-1 expression in microvascular pulmonary endothelial cells. Exp Toxic Pathol 2004;55:497-503.

Fischer U, Hempel U, Becker D, Bierbaum S, Scharnweber D, Worch H, Wenzel K-W. Transforming growth factor beta1 immobilized adsorptively on Ti6Al4V and collagen type I coated Ti6Al4V maintains its biological activity. Biomaterials 2003;24:2631-2641.

Geißler U, Hempel U, Wolf C, Scharnweber D, Worch H, Wenzel K-W. Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts. J Biomed Mater Res 2000;51:752-760.

Hempel U, Poppe M, Reinstorf A, Fischer U, Gelinsky M, Pompe W, Wenzel K-W. Proliferation and differentiation of osteoblasts on Biocement D modified with collagen type I and citric acid. J Biomed Mater Res B Appl Biomater 2004;71:130-143.

Menschikowski M, Hagelgans A, Hempel U, Siegert G. Glycogen synthase kinase-3beta negatively regulates group IIA phospholipase A(2) expression in human aortic smooth muscle and HepG2 hepatoma cells. FEBS Lett 2004;577:81-86.

Menschikowski M, Hagelgans A, Heyne B, Hempel U, Neumeister V, Goez P, Jaross W, Siegert G. Statins potentiate the IFN-gamma-induced upregulation of group IIA phospholipase A2 in human aortic smooth muscle cells and HepG2 hepatoma cells.

Biochim Biophys Acta 2005;1733:157-71.

Pompe W, Worch H, Epple M, Friess W, Gelinsky M, Greil P, Hempel U, Scharnweber D, Schulte K. Functionally graded materials for biomedical applications. Materials Sci Eng A 2003;362:40-60.

Reinstorf A, Ruhnow M, Gelinsky M, Pompe W, Hempel U, Wenzel K-W, Simon P. Phosphoserine – a convenient compound for modification of calcium phosphate bone cement collagen composites. J Mater Sci Mater Med 2004;15:451-455.

Scheibe R, Dieter P, Hempel U, Wenzel K-W. Activation of MAPK p42/ p44, MAPK p38, and JNK in primary rat hepatic stellate cells. Cells of the hepatic sinusoid 2001;8:262-263.

Schwab W, Friess U, Hempel U, Schulze E, Makita Z, Kasper M, Simank H-G. Immunohistochemical demonstration of Nε-(carboxymethyl)lysine protein adducts in normal and osteoarthritic cartilage. Histochem Cell Biol 2002;117:541-546.

Zahanich I Graf EM, Heubach JF, Hempel U, Boxberger S, Ravens U. Molecular and functional expression of voltage-operated calcium channels during osteogenic differentiation of human mesenchymal stem cells. J Bone Miner Res 2005; 20:1637-46.