Automated DNA Sequencing [G14]

Lecturer:  Dr. Beatrice Weber
Script:       Script WS 2016/17

Content:
The course gives an introduction into automated DNA sequencing. Participants investigate novel plant DNA sequences from current research projects by the application of the chain-termination-sequence method (Sanger sequencing). Two different sequencing strategies will be used. Direct sequencing of short plasmid inserts will be carried out using plasmid specific M13 primer, while sequencing of large BAC inserts will be performed with sequence specific primer. Students will practise the usage of an automated DNA sequencer and the computer based analysis of the resulting sequences and will be trained in the generation of sequence-based primer. They will learn the isolation of plasmid DNA, restriction by endonucleases, gel electrophoresis, the synthesis of labelled DNA and PCR. At the end of the course all participants will discuss their experimental results in a seminar.