Equipment
Inhaltsverzeichnis
Molecular Biology and Biotechnology
DNA Equipment
GelDoc
Models:
- VILBER Smart Imaging Quantum
- BIO-RAD Gel Doc 1000
Specifications: On-Click-to-the-Image, auto-focus and -lighting, 5 megapixels resolution
Applications: gel imaging system for documentation of nucleic acid and protein distribution in various gel types (polyacrylamide, agarose) using fluorescence stain imaging (GFP, Coomassie blue).
PCR/Thermocycler
Model:
- BIO-RAD T100™ Thermal Cycler
Amount: 6
Specifications: 96-well block format, sample volume of 15–100 μL, temperature accuracy of ± 0.5 °C, maximum ramp rate of 4 °C/sec, temperature range from 4–100 °C.
Application: laboratory device that independently carries out the temperature cycles of a polyermase chain reaction (PCR) using a highly sensitive heating block. Replication of bacterial genetic material from E. coli and Streptomyces.
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Expression and Cultivation
Autoclave
Models:
- VWR Vapor-Line 135-M
- VARIOKLAV Typ 500
- SANOclav/CertoClav
- Tuttnauer AUtoklav 3170ELV
Specifications: large- and small-scale fully automated and programmed autoclaves.
Applications: sterilization of culture medium (LB, TB, 2YT) and equipment for biochemical applications.
Bioreactor
Model:
- sartorius stedim biotech BIOSTAT® A plus
- INFORS HT Labfors 5 Benchtop Bioreactor
Specifications: simple and automated aeration control (up to five gases) as well as light control for photoautotrophic growth conditions, integrated chiller, operating temperature 0–40 °C.
Applications: cell cultivation and microbial fermentation.
Centrifuge
Models:
- Hettich universal 30RF
- Thermo Scientific Heraerus Multifuge X3R / Sorvall Legend XTR
- VWR ;EGA STAR 1.6R
Specifications: high speed centrifuges up to 22.000 rpm, included refrigeration system.
Applications: separation of insoluble components from suspensions.
Incubator
Models:
- INFORS HT Multitron Standard
- INFORS HT Ecotron / Minitron
Amount: 9
Specifications: fundamental shaker including temperature and speed control, cooling function to run system below room temperature and partly light contol for photoautotrophic growth conditions.
Applications: protein expression and media development.
Low Temperature Freezer
Models:
- Thermo Scientific Froma 900 Series
- ARCTIKO
- SANYO
Specifications: –80 °C low temperature freezers including emergency battery for offline power supply.
Applications: storage of sensitive and temperature unstable biochemical (DNA, enzymes, proteins, stock solutions) and chemical compounds (reactive intermediates, final natural products, easily isomerizable derivatives).
SDS-PAGE/Electrophoresis
Producers:
- BIO-RAD
- amersham parmacia biotech EPS 301
Specifications: power supply up to 300 V, possible parallel mode.
Applications: submarine, mini vertical and standard vertical gel electrophoresis as well as semi dry and mini tank blotting.
Sonicator
Models:
- BRANSON Digital Sonifier 450-D
- BRANSON SONOPULS
Amount: 3
Specifications: multiple modes of operation, including continuous, pulsed, timed, and temperature end-point.
Applications: cell disruption, homogenization, cell lysing, processing DNA and proteins.
SpeedVac
Model:
- Eppendorf Concentrator 5301
Specifications: sample capacity of 144 vials, integrated membrane vacuum pump.
Applications: concentration of biochemical samples, removing of small amount of organic solvents.
Sterile Bench
Models:
- Heraeus HERAsafe HS 9
Amount: 5
Specifications: class II safety cabinet including full electronic monitoring system to control air stream and filter capacity. Aerosol-tight front window for safe working.
Applications: inoculation of cell cultures (e.g. on agar plates), production of sterile stock solutions.
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Chemical Synthesis
Freeze Dryer
Model:
- Christ Alpha 2-4
Specifications: condensator temperature down to –80 °C, capacity of around 4 kg ice, possibility to attach 12 flasks at the same time.
Applications: removing of water through sublimation under low pressure, ideal for removing of eluent after HPLC or MPLC purification.
High Perfomance Balance
Models:
- sartorius MC1 RC 210 P
- sartorius BP 221 S
- sartorius BP 3100 S
Specifications: high perfomance balances capable to exactly weigh in milligram scale.
Applications: production of stock solution, yield determination of small-scale reactions.
Large-Scale Dewar
Models:
- cryopAL TP100
- VWR LD-50
Specifications: 100 L and 50 L dewar for storage of liquid nitrogen.
Applications: base of the cooling traps of the schlenk systems, high-speed freezing of sensible samples.
Microwave System
Model:
- Anton Paar MONOWAVE 50
Specifications: synthesis reactor with maximum temperature of 250 °C, maximum pressure of 20 bar.
Applications: performing of cross-couplings like Suzuki reactions or Pechmann condensations.
Rotary Evaporator
Models:
- Pumps: KNF SC920, SC920G, SC950 SCC950
- Rotary Evaporators from: Büchi, Heidolph, KNF, VWR
Specifications: membrane pumps with maximum vacuum pressure of 2 mbar, SCC950 capable of operating two evaporators.
Applications: concentration of samples by evaporating solvent using reduced pressure and water heating bath.
Schlenk Line System
Models:
- Ilmvac PK2DC
- Vacuubrand RZ2/5/6
- VWR RV3+
Specifications: high perfomance pump with maximum vacuum of 10-5 mbar, schlenk line with direct argon supply.
Applications: performing of reactions under exclusion of air and moisture.
Syringe Pump
Model:
- HLL LA-30
Specifications: syringe pump with maximum volume up to 60 mL, maximum pressure of 2 bar.
Applications: long-term addition of reactants.
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Small Molecule and Protein Chromatography
Liquid Chromatography
Fraction Collecting System
Model:
- Knauer Foxy R1 - TELEDYNE ISCO
Specifications: one rack fraction collector with up to 96 test tubes, collects uniform fractions by time, drop count, or pumped volume.
Applications: performing standard column chromatography (silica gel, size-exclusion) with automated collecting.
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High Performance Liquid Chromatography (HPLC)
Analytical HPLC
Models:
- Knauer Azura system: AS 6.1L sampler, P 6.1L pump, DAD 2.1L detector
- Jasco system: 2 PU-1580 Intelligent pumps, AS-15500 Intelligent sampler, HG-1580-32 Dynamic mixer, UV-1575 Intelligent UV/VIS-Detector
Amount: 5
Specifications: high perfomance system running with H2O/acetonitrile, usage of Eurospher C18 and C8 columns.
Applications: from basic reaction control up to quantitative bio assay analysis.
Preparative HPLC
Models:
- Knauer system: GAT autosampler, Azura P6.1L pump, degasser, UV detector K-2501, Foxy R1 fraction collector
- Jasco system: 2 PU-2086 Plus Intelligent pumps, MIKA 100 Dynamic mixing chamber, UV-1575 Intelligent UV/VIS detector
Applications: quantitative HPLC for high-end purification of crude reaction outcome or biochemical extracts.
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Medium Pressure Liquid Chromatography (MPLC)
Model:
- Reveleris® X2 MPLC system Grace
- Büchi Pure C-810 Flash
Specifications: multi-wavelength detector, up to 1 gram loading and 4 different solvents, automated peak detection and fraction collecting.
Application: purification of complex compound mixtures of medium-scale reactions (500–1000 mg).
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Fast Protein Liquid Chromatography (FPLC)
Models:
- GE ÄKTA™ pure (fraction collector F9-R)
- FE ÄKTA™ Start (fraction collector Frac30)
Amount: 5
Specifications: multi-wavelength detector, pH monitoring, up to 20 MPa maximum pressure, multiple sample injection.
Applications: purification of proteins and peptides from microgram levels to grams of target product by affinity chromatography, size exclusion chromatography and/or ion exchange chromatography.
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Analytics
Mass Spectrometry (MS, LC-MS)
Model:
- Advion expressionL CMS system: ESI single-quadrupole mass analyzer, Peak Scientific N118LA nitrogen generator, Edwards RV12 high vacuum pump
- Further accessories: Advion plate Express (evaluation of TLC plates), connectable to HPLC system (Knauer Azura)
Applications: MS determination of pure samples via direct injection, analysis of reactions via LC-MS measurements.
High-Resolution Mass Spectrometry (HRMS, LC-HRMS)
Model:
- Bruker impact II ultra-high resolution QTOF quadrupol time-of-flight analyzer, cmc nitrogen membrane generator NGM 22, Agilent high vacuum pump
- further accessories: connectable to HPLC system (Bruker Elute UHPLC with HPG 1300 pump)
Applications: HRMS determination of pure samples via direct injection, analysis of reactions and culture extracts via LC-MS measurements.
Polarimeter
Model:
- KRÜSS P3000
Applications: measurements of the angle of rotation for quantitative determination of the concentration of optically active substances in solutions.
UV Spectrometry
Model:
- biochrom Ultraspec 2100 pro
Amount: 10
Specifications: preprogrammed measuring methods, multiwavelength mode.
Applications: Bradford and Ellmann assay, determination of peptide/protein concentrations, kinetic studies.