Scientistshavelongarguedoverwhichcellsenablesalamanderstogrowbacklostlimbs.Bytrackinglineagesandcharacterisingindividualcells,researcherscouldnowshowthatconnectivetissuecellsdevelopstem-cell-likepropertiesandunderlietheregenerationoflegs.Thefindingswerepublishedinthejournal“Science” .

Among all four-legged animals, the ability of salamanders to replace lost limbs even as adult animals is unique. This has made some species of salamander, such as the Mexican axolotl (Ambystoma mexicanum), popular model organisms for studying regeneration. If an axolotl loses a limb, then cells from near the stump accumulate and form a tissue called blastema.

The blastema can grow back a fully functional limb composed of many different tissues and cell types, such as muscles, neurons or connective tissue. Until now, it was unclear how mature tissue can produce blastema cells. A study published in “Science” took a closer look at connective tissue cells to shed light on this issue.

There were two possible models leading to the formation of a blastema: either stem cells sit dormant within the connective tissue and wait for when they are needed; or mature connective tissue cells respond to the loss of a limb by “de-differentiating” into limb progenitor cells similar to those in an embryo. So far, isolating a blastema precursor cell and tracking the fate of its lineage in an adult axolotl was not possible due to the variety of  tissues that exist in a limb. An international team of scientists in Vienna, Leipzig and Dresden have now overcome this hurdle.

The scientists created strains of genetically marked axolotls that expressed molecular labels associated with connective tissue cells. They also applied single-cell RNA sequencing to analyse the activity of different genes in specific cells. Combined, the two approaches allowed them to track origin and fate of blastema-precursors and characterise their molecular profiles through the course of limb regeneration.

A particular challenge was posed by the creation of the transgenic axolotl lines that molecularly labels individual tissue types. Transgenesis in axolotls is time consuming, as it takes a year until an axolotl embryo reaches adulthood and can reproduce. The experiments were carried out at the Research Institute of Molecular Pathology (IMP) in Vienna, the Max Planck Institute for Evolutionary Anthropology (MPI-EVA) in Leipzig, and the Center for Regenerative Therapies Dresden (CRTD).

The scientists showed that blastema progenitors originate from mature fibroblasts. If a limb is lost, then these mature cells “de-differentiate” into progenitor cells that are similar to the cells found in embryonic limb buds. The experiments could not find any indication for the presence of pre-existing progenitor cells, which some scientists had thought were the reason for the axolotl’s outstanding gift to regrow limbs and organs.

The findings are ground-breaking, not only because they settle a long-standing debate in developmental biology, but also because they have high relevance for understanding regeneration in mammals. There, too, fibroblasts respond to injuries by differentiating – they turn into myofibroblasts, which then form scars. What makes axolotl fibroblasts different, what empowers them to develop stem-cell properties and replace complex body parts?

Finding the answer will be the next chapter in the scientific journey towards understanding regeneration.

Quotes

Prayag Murawala, postdoctoral researcher in the lab of Elly Tanaka at the IMP:
“When we tried to analyse the fate of cells in regenerating limbs, it used to be like making a fruit juice with a bowl of fruit without knowing what kinds of fruit are inside”, says Prayag Murawala of the Research Institute of Molecular Pathology (IMP), one of the authors. “but now we can pick apples and make an apple-juice, or we can pick cherries and make cherry- juice from this mix-fruit bowl. Using this new level of resolution, we showed that there is no ‘magic cell’ that axolotls would have and that mammals would not have. Both rely on fibroblasts in case of an injury, but one can regrow organs, whereas the other forms fibrotic scars.”

Tobias Gerber, graduate student in the lab of Barbara Treutlein at the MPI for Evolutionary Anthropology:
“I have always been amazed by the enormous regeneration capacity of axolotls and it was great to work together with the Tanaka lab to study this fascinating process”, says Tobias Gerber of the MPI for Evolutionary Anthropology in Leipzig. "Thanks to a new method that allows you to measure gene expression in thousands of single cells, we were able to study how individual axolotl cells can use their genome to help regenerate an arm. We found that the axolotl can take a mature cell and turn it into an embryonic-like precursor cell and then replay development to grow a new arm.”

Dunja Knapp, postdoc at DFG Research Center for Regenerative Therapies Dresden (CRTD):
“When we started this work, it was unclear whether blastema-like cells exist in the mature uninjured limbs ready to get activated in case of an injury. Now we carefully looked through thousands of cells in uninjured limbs and haven't found a single cell like it. This indicated that an injury stimulates reprogramming of mature cells in the limbs. Now it will be important to figure out if an injury can induce similar changes in mature mammalian cells.”

Original publication:
Gerber, T., Murawala, P., Knapp, D., Masselink, W., Schuerz, M., Hermann, S., Gac-Santel, m., Nowoshilow, S., Kagejama, J., Khattak, S., Currie, J., Camp, J. G., Tanaka, E. M., Treutlein, B.: “Single-cell transcriptomics uncovers convergence of cell identities during axolotl limb regeneration”. Science, 27 September 2018. http://science.sciencemag.org/content/early/2018/09/26/science.aaq0681

Illustrations & Media: The image of this press release and further illustrations including a video of a regenerating axolotl limb can be downloaded from the IMP website at www.imp.ac.at/supplements.

About the IMP
The Research Institute of Molecular Pathology (IMP) in Vienna pursues world-class research in basic molecular biology. It is located at the Vienna BioCenter and largely sponsored by Boehringer Ingelheim. With over 200 scientists from 40 countries, the IMP is committed to scientific discovery of fundamental molecular and cellular mechanisms underlying complex biological phenomena. www.imp.ac.at

About the MPI for Evolutionary Anthropology
The Max Planck Institute for Evolutionary Anthropology unites scientists with various backgrounds (natural sciences and humanities) whose aim is to investigate the history of humankind from an interdisciplinary perspective with the help of comparative analyses of genes, cultures, cognitive abilities and social systems of past and present human populations as well as those of primates closely related to human beings. www.eva.mpg.de

About the CRTD
Founded in 2006, the DFG Research Center for Regenerative Therapies Dresden (CRTD), Cluster of Excellence at the TU Dresden has passed the second phase of the Excellence Initiative which aims to promote top-level research and improve the quality of German universities and research institutions. The goal of the CRTD is to explore the human body's regenerative potential and to develop completely new, regenerative therapies for hitherto incurable diseases. The key areas of research include haematology and immunology, diabetes, neurodegenerative diseases, and bone regeneration. At present, eight professors and twelve group leaders are working at the CRTD – integrated into an interdisciplinary network of 87 members at seven different institutions within Dresden. In addition, 21 partners from  industry are supporting the network. The synergies in the network allow for a fast translation of results from basic research to clinical applications. The CRTD is part of the Center for Molecular and Cellular Bioengineering (CMCB). www.crt-dresden.de  www.tu-dresden.de/cmcb