Equipment
Table of contents
Equipment belonging to our group
Ultrasound Cell Disruptor
![A device to lyse cells with the help of ultrasound in a water bath](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/BioRuptor.JPG/@@images/a862e4cf-a800-454e-99bd-7f23653eb3e6.jpeg)
Bioruptor from Diagenode
Company: Diagenode
Model: Bioruptor
Volume: 1.5/2 mL and 15 mL tubes
location: room 134 (coldroom)
Cell disruption and DNA shearing with ultrasound in a water bath; application: e.g. chromartin immunoprecipitation (ChIP)
Sonifier
![A device to lyse cells with the help of ultrasound originating from a metal tip in the cell solution](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/Sonifier.JPG/@@images/96a49634-c42e-4649-84d9-39111bbc98d9.jpeg)
Sonifier W-450-D from Branson
Company: Branson
Model: W-450-D
location: room 134 (coldroom)
cell lysis with ultrasound, 3 different tip sizes, with soundinsulation cabinet
Evaporator
![It is a device to evaporate solvents in parallel under vacuum and during centrifugation in parallel from several samples](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/GeneVac.JPG/@@images/12d4e5af-5ebe-4f7d-95f6-a16599a6d3d2.jpeg)
EZ-2 Plus from GeneVac
Company: GeneVac
Model: EZ-2 Plus
Parallel evaporation of pure and mixed solvents under vacuum and during centrifugation, adaptors for 2 mL and 20 mL glass tubes; different programs for varying solvent groups; automatic stop when dry
Pulse Field Electrophoresis
![Shows the puls filed electrophoresis machine from the company BioRad called CHEF Mapper](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/CHEF-Mapper.jpg/@@images/17171129-6202-4a86-9f88-de1fd1a35679.jpeg)
CHEF Mapper from BioRad
Company: BioRad
Model: CHEF Mapper® XA
It is used to resolve chromosomal sized DNAs. Alternating the electrical field between spatially distinct pairs of electrodes causes magabase size DNAs to re-orient and move at different speeds through the pores in an agarose gel. DNAs ranging from from 100 bases to over 10 megabases may be effectively resolved.
DNA shearing
![A machine which can physically shear DNA and the laptop to operate the device](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/Hydroshear.JPG/@@images/36632c1d-7379-498c-87e7-91ccd51b13cf.jpeg)
Hydroshear from DigiLab
Company: DigiLab Genomic Solutions, Model: HydroShear
The DNA is physically sheared by pressing the solution through a valve. The fragment size can be adjusted.
UV Radiation
![A device to irradiate things with UV light](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/Stratalinker.JPG/@@images/6053fa7e-cfe2-424d-bf50-246bd0a818d1.jpeg)
UV Stratalinker 2400 from Stratagene
Company: Stratagene
Model: UV Statalinker 2400
application: DNA crosslinking on nylon membranes, UV mutagenesis in microorganisms
Electroporator
![A device to parallel transform DNA into several cell batches with the help of an electrical field](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/Electroporator.JPG/@@images/b864cbc2-91ab-48b0-b112-fe3df2836802.jpeg)
Electroporator ECM 360 from BTX Harvard Apparatus
Company: BTX Harvard Apparatus
Model: ECM 360 with modul HT200 for high-throughput electroporation (25 or 96 wells)
Plattform equipment in our resonsibility
PCR
![A PCR machine](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/Mastercycler-epGradient-S.JPG/@@images/7b33af92-0ecc-49a6-ac3f-47835514969b.jpeg)
Mastercycler epGradient S with operating panel from Eppendorf
Company: Eppendorf
Model: Mastercycler epGradient S, five machines
location: room 123
RT-qPCR
![A RT-qPCR machine](https://tu-dresden.de/cmcb/biotec/forschungsgruppen/stewart/resources/bilder/equipment/RT-PCR.JPG/@@images/b5fdb23e-9146-4feb-bae6-2035bf457679.jpeg)
RT-qPCR system Mx30005P from Stratagene
Company: Stratagene
Models: 1 x Mx3000P, 2 x Mx3005P
location: room 123