Equipment
Inhaltsverzeichnis
Equipment belonging to our group
Ultrasound Cell Disruptor

Bioruptor from Diagenode
Company: Diagenode
Model: Bioruptor
Volume: 1.5/2 mL and 15 mL tubes
location: room 134 (coldroom)
Cell disruption and DNA shearing with ultrasound in a water bath; application: e.g. chromartin immunoprecipitation (ChIP)
Sonifier

Sonifier W-450-D from Branson
Company: Branson
Model: W-450-D
location: room 134 (coldroom)
cell lysis with ultrasound, 3 different tip sizes, with soundinsulation cabinet
Evaporator

EZ-2 Plus from GeneVac
Company: GeneVac
Model: EZ-2 Plus
Parallel evaporation of pure and mixed solvents under vacuum and during centrifugation, adaptors for 2 mL and 20 mL glass tubes; different programs for varying solvent groups; automatic stop when dry
Pulse Field Electrophoresis

CHEF Mapper from BioRad
Company: BioRad
Model: CHEF Mapper® XA
It is used to resolve chromosomal sized DNAs. Alternating the electrical field between spatially distinct pairs of electrodes causes magabase size DNAs to re-orient and move at different speeds through the pores in an agarose gel. DNAs ranging from from 100 bases to over 10 megabases may be effectively resolved.
DNA shearing

Hydroshear from DigiLab
Company: DigiLab Genomic Solutions, Model: HydroShear
The DNA is physically sheared by pressing the solution through a valve. The fragment size can be adjusted.
UV Radiation

UV Stratalinker 2400 from Stratagene
Company: Stratagene
Model: UV Statalinker 2400
application: DNA crosslinking on nylon membranes, UV mutagenesis in microorganisms
Electroporator

Electroporator ECM 360 from BTX Harvard Apparatus
Company: BTX Harvard Apparatus
Model: ECM 360 with modul HT200 for high-throughput electroporation (25 or 96 wells)
Plattform equipment in our resonsibility
PCR

Mastercycler epGradient S with operating panel from Eppendorf
Company: Eppendorf
Model: Mastercycler epGradient S, five machines
location: room 123
RT-qPCR

RT-qPCR system Mx30005P from Stratagene
Company: Stratagene
Models: 1 x Mx3000P, 2 x Mx3005P
location: room 123