Adaptive 4D cell excitation for Optogenetics and 1P/2P Microscopy
Spatial light modulators offer a variety of possibilities and functionalities for targeted light field shaping (see general point "Adaptive Optical Systems"). They can be used to create single or multiple foci in three dimensions, which can specifically excite 1P or 2P fluorescence in cells, or excite an optogenetic reaction.
Example: Setup for holographic optogenetic stimulation
Sketch of the stimulation setup
- 450nm laser diode suited for ChR2 activation
- Ferroelectric spatial light modulator (Forth Dimension Displays) for display of FullHD computer generated holograms
- Up to 1.7kHz frame rate for fast kinetics
- Multiple simultaneous spot stimulation at single cell resolution
-Lateral and axial scanning w/o moving parts-Cellular lateral resolution with long working distance (>30mm)
- Light source easily interchangeable for different (colour-) channels
- GUI for calibration and stimulation protocols
[1] F. Schmieder, S. D. Klapper, N. Koukourakis, V. Busskamp, J. Czarske, "Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator-Based Holographic Illumination", Applied Sciences 8, Special Issue Applications of Digital Holographic Microscopy (2018).