J. Clement, F. v. Eggeling: The dynamic of magnetic field driven interaction of superparamagnetic nanoparticles with cellular barriers in the presence of therapeutic agents
Description
In view of the incremental use of magnetic hybrid materials for biomedical applications a deeper understanding of the fundamental processes is needed. Especially the dynamics of nanoparti-cle–cell interactions on a molecular level are of particular importance.
Therefore the suggested proposal will cover the following aspects in more detail, (i) the shape, charge and chemical compounds of magnetic hybrid materials and their influence on the interac-tion process with cells; (ii) the role of the biologi-cal identity, which is dependent on the composi-tion of the biological fluids and is represented by the protein corona; (iii) the contacting of the magnetic hybrid materials with the cell surface and its consequences for nanoparticles relaxation behavior and the structure of the cell surface; (iv) the intracellular processing and the fate of the magnetic hybrid materials; (v) the consequences of the afore mentioned processes for the appli-cation of the magnetic hybrid materials during pregnancy or during therapeutically interventions, e.g. cancer therapy.
HBMEC cells are incubated with starch coated iron oxide nanoparticles (nanoscreenMAG/R-D, chemicell, Berlin). Incorporated nanoparticles are preferentially located close to the cell nucleus (blue). The cytoskeleton is visualized by phalloidin staining (gr
Project Manager
Dr. Joachim Clement, Universitätsklinikum Jena
Prof. Dr. Ferdinand von Eggeling, Universitätsklinikum Jena
Staff
Christine Gräfe, Universitätsklinikum Jena
Grant period
2013 -
Publications
[1] Gräfe, C., Slabu, I., Wiekhorst, F., Bergemann, C., von Eggeling, F., Hochhaus, A., Trahms, L., Clement, J.H. 2016. A model system for quantitative study of iron oxide nanoparticle passage through an endothelial cell layer. Phys. Med. Biol. 61:3986-4000
[2] Gräfe, C., Weidner, A., von der Lühe, M., Bergemann, C., Schacher, F.H. Clement, J.H., Dutz, S. 2016. The Protein Corona as a Regulator of Nanoparticle-Cell Interactions. Int. J. Biochem. Cell Biol. 75: 196-202
[3] Zhou, M., Liebert, T., Müller, R., Dellith, A., Gräfe, C., Clement, J.H., Heinze, T. 2015. Magnetic biocomposites for remote melting. Biomacromolecules 16: 2308-2315
[4] Weidner, A., Gräfe, C., von der Lühe, M., Remmer, H., Clement, J.H., Eberbeck, D., Ludwig, F., Müller, R., Schacher, F.H., Dutz, S. 2015. Preparation of core-shell hybrid materials by producing a protein corona around magnetic nanoparticles. Nanoscale Research Letters: DOI 10.1186/s11671-015-0992-2
Contact
Universitätsklinikum Jena
Abt. Hämatologie und Internistische Onkologie
Am Klinikum 1
07747 Jena