S. Dutz: Formation kinetics, stability, and field-mediated interaction with biological systems of a protein corona generated on magnetic na-noparticles
Description
This project focuses on the controlled formation of a protein corona on magnetic nanoparticles (MNP), the influence of different factors onto this process, as well as the influence of the formed corona on interac-tions of these hybrid particles with a biological sys-tem. We will therefore synthesize core-shell hybrid particles, consisting of a magnetic core and a polymer shell of varying charge. These hybrid particles then are incubated in fetal calve serum (FSC) for the formation of a protein corona. We are particularly interested in elucidating the interaction of particles after incubation with different biological systems. This is investigated by monitoring the contact between MNP@corona hy-brid particles with blood, different cell lines, and ani-mals – in certain cases also under the influence of an external field or field gradient. Suitable methods are µ-rheology, established cell toxicity assays, as well as 2D and 3D microscopic techniques for cell lines and animals. Further, we are also interested in how such particles have to be prepared or stored for in-vivo applications.
Another important goal of this project is to gain in-depth understanding about the kinetics of protein corona formation. For this, key technologies are mag-netic relaxometry, AC-susceptometry, and small angle X-ray scattering (SAXS) – all of which are highly sensi-tive towards small changes in hydrodynamic diameter and, hence, the growth of the protein corona.
During the first period of this priority program, we established techniques for a defined and reproducible corona formation. We are now interested in probing mechanical properties as well as the (chemical) stabil-ity of the protein corona. This is realized by incorpo-rating two dyes representing a FRET pair (Förster res-onance energy transfer) within polymer shell and pro-tein corona. As long as the corona is intact, both counterparts are in close proximity and FRET can be detected. Upon release of the protein corona, this energy transfer disappears and we plan to use this for probing corona stability under different conditions, including in-vivo experiments. In another part of this project, we investigate the effect of a compartmental-ized polymer shell, e.g. through the presence of hy-drophobic and hydrophilic patches, onto the protein corona formation. We anticipate that using core-shell particles featuring such patches influences both the mechanism of protein adsorption as well as the amount and composition of the corona being formed.
Project Manager
Dr.-Ing. Silvio Dutz, TU Ilmenau
Staff
Dipl.-Ing. Andreas Weidner
Grant period
2016 - 2017
Publications
[1] M. v.d. Lühe, U. Günther, A. Weidner, C. Gräfe, J.H. Clement, S. Dutz, F.H. Schacher. SPION@Polydehydroalanine Hybrid Particles. RSC Advances 5: 31920–31929 (2015).
[2] A. Weidner, C. Gräfe, M. v.d. Lühe, H. Remmer, J.H. Clement, D. Eberbeck, F. Ludwig, R. Müller, F.H. Schacher, S. Dutz. Preparation of Core-Shell Hybrid Materials by Produc-ing a Protein Corona around Magnetic Nanoparticles. Nanoscale Research Letters 10: 282 (2015).
[3] C. Gräfe, A. Weidner, M. v.d. Lühe, C. Bergemann, F.H. Schacher, J.H. Clement, S. Dutz. Intentional formation of a protein corona on nanoparticles - Serum concentration affects protein corona mass, surface charge, and nanoparticle-cell interaction. The International Journal of Biochemistry & Cell Biology (2016) dx.doi.org/10.1016/j.biocel.2015.11.005
Contact
TU Ilmenau
Fakultät Informatik und Automatisierung
Institut für Biomedizinische Technik und Informatik
Gustav-Kirchhoff-Straße 2
98693 Ilmenau