Publikationen
2023
Poetsch MS, Palus S, Van Linthout S, von Haehling S, Doehner W, Coats AJS, Anker SD, Springer J. The small molecule ACM-001 improves cardiac function in a rat model of severe cancer cachexia. Eur J Heart Fail. 2023; Epub ahead of print. doi: 10.1002/ejhf.2840.
Oeing CU, Pepin ME, Saul KB, Agircan AS, Assenov Y, Merkel TS, Sedaghat-Hamedani F, Weis T, Meder B, Guan K, Plass C, Weichenhan D, Siede D, Backs J. Indirect epigenetic testing identifies a diagnostic signature of cardiomyocyte DNA methylation in heart failure. Basic Res Cardiol, 2023;118(1):9. doi: 10.1007/s00395-022-00954-3.
Cachorro E.*, Günscht M.*, Schubert M. *, Sadek M.S., Siegert J., Dutt F., Bauermeister C., Quickert S., Berning H., Nowakowski F., Lämmle S., Firneburg R., Luo X., Künzel S.R., Klapproth E., Mirtschink P., Mayr M., Dewenter M., Vettel C., Heijman J., Lorenz K., Guan K., El-Armouche A., Wagner M., Kämmerer S. CNP promotes antiar-rhythmic effects via phosphodiesterase 2. Circ Res., 2023; 132(4):400-414. doi: 10.1161/CIRCRESAHA.122.322031.
2022
Klapproth E, Witt A, Klose P, Wiedemann J, Vavilthota N, Künzel SR, Kämmerer S, Günscht M, Sprott D, Lesche M, Rost F, Dahl A, Rauch E, Kattner L, Weber S, Mirtschink P, Kopaliani I, Guan K, Lorenz K, Saftig P, Wagner M, El-Armouche A. Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction. Nat Commun, 2022;13(1):7648. doi: 10.1038/s41467-022-35331-0.
Künzel K, Künzel S, Guan K. Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes. Heliyon, 2022;8:e10365. doi: 10.1016/j.heliyon.2022.e10365
Weninger G, Pochechueva T, El Chami D, Luo X, Kohl T, Brandenburg S, Urlaub H, Guan K, Lenz C, Lehnart SE. Calpain Cleavage of Junctophilin-2 Generates a Spectrum of Calcium-dependent Cleavage Products and DNA-rich NT1-Fragment Domains in Cardiomyocytes. Sci Rep, 2022;12(1):10387. doi: 10.1038/s41598-022-14320-9.
Murganti F, Derks W, Baniol M, Simonova I, Trus P, Neumann K, Khattak S, Guan K, Bergmann O. FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes. Front Cardiovasc Med, 2022;9:840147. doi: 10.3389/fcvm.2022.840147.
Poetsch MS, Strano A, Guan K. Human induced pluripotent stem cells: From cell origin, genomic stability and epigenetic memory to translational medicine. Stem Cells, 2022;sxac020. doi: 10.1093/stmcls/sxac020.
Binder P, Nguyen B, Collins L, Zi M, Liu W, Christou F, Luo X, Hille SS, Frey N, Cartwright EJ, Chernoff J, Mueller OJ, Guan K, Wang X. Pak2 regulation of Nrf2 serves as a novel signalling nexus linking ER stress response and oxidative stress in the heart. Front Cardiovasc Med, 2022;9:851419. doi: 10.3389/fcvm.2022.851419.
Li W, Luo X, Poetsch MS, Oertel R, Nichani K, Schneider M, Strano A, Hasse M, Steiner RP, Cyganek L, Hettwer K, Uhlig S, Simon K, Guan K*, Schubert M*. Synergistic Adverse Effects of Azithromycin and Hydroxychloroquine on Human Cardiomyocytes at a Clinically Relevant Treatment Duration. Pharmaceuticals. 2022;15(2):220. doi: 10.3390/ph15020220.
2021
Schüß C*, Vu O*, Schubert M*, Du Y, Mishra NM, Tough IR, Stichel J, Weaver CD, Emmitte KA, Cox HM, Meiler J, Beck-Sickinger AG. Highly Selective Y4 Receptor Antagonist Binds in an Allosteric Binding Pocket. J Med Chem., 2021;64(5):2801-2814.
doi: 10.1021/acs.jmedchem.0c02000.
Li W, Luo X, Ulbricht Y, Guan K. Blebbistatin protects iPSC-CMs from hypercontraction and facilitates automated patch-clamp based electrophysiological study. Stem Cell Res, 2021;56:102565. doi: 10.1016/j.scr.2021.102565.
Künzel SR, Hoffmann M, Weber S, Künzel K, Kämmerer S, Günscht M, Klapproth E, Rausch JSE, Sadek M Kolanowski T, Meyer-Roxlau S, Piorkowski C, Tugtekin SM, Rose-John S, Yin X, Mayr M, Kuhlmann JD, Wimberger P, Grützmann K, Herzog N, Küpper JH, O'Reilly M, Kabir SN, Sommerfeld LC, Guan K, Wielockx B, Fabritz L, Nattel S, Ravens U, Dobrev D, Wagner M, El-Armouche A. Diminished PLK2 Induces Cardiac Fibrosis and Promotes Atrial Fibrillation. Circ Res., 2021;129(8):804-820. doi: 10.1161/CIRCRESAHA.121.319425.
Marchal GA, Jouni M, Chiang DY, Pérez-Hernández Duran M, Podliesna S, Yu N, Casini S, Potet F, Veerman CC, Klerk M, Lodder EM, Mengarelli I, Guan K, Vanoye CG, Rothenberg E, Charpentier F, Redon R, George A Jr, Verkerk AO, Bezzina CR, MacRae CA, Burridge P, Delmar M, Galjart NJ, Portero V, Remme CA. Targeting the Microtubule EB1-CLASP2 Complex Modulates NaV1.5 at Intercalated Discs. Circ Res., 2021;29(3):349-365.
doi: 10.1161/CIRCRESAHA.120.318643.
Yi C, Spitters TW, Al-Far EAA, Wang S, Xiong T, Cai S, Yan X, Guan K, Wagner M, El-Armouche A, Antos CL. A calcineurin-mediated scaling mechanism that controls a K+-leak channel to regulate morphogen and growth factor transcription. Elife, 2021;10:e60691. doi: 10.7554/eLife.60691
Li W, Henze S, Luo X, Ulbricht Y, Richter A, Di Donato N, Wilde AAM, Guan K. Generation of iPSC lines from CPVT patient carrying heterozygous mutation p.A2254V in the ryanodine receptor 2 gene. Stem Cell Res., 2021;53:102259.
doi: 10.1016/j.scr.2021.102259
Verkerk AO, Knottnerus SJG, Portero VM, Bleeker JC, Ferdinandusse S, Guan K, IJlst L, Visser G, Wanders RJA, Wijburg FA, Bezzina CR, Mengarelli I, Houtkooper RH. Electrophysiological abnormalities in VLCAD deficient hiPSC-cardiomyocytes do not improve with carnitine supplementation. Front Pharmacol., 2021;11:616834.
doi: 10.3389/fphar.2020.616834
Bornstein SR, Guan K, Brunßen C, Mueller G, Kamvissi-Lorenz V, Lechler R, Trembath R, Mayr M, Poston L, Sancho R; IRTG Study Group:, Ahmed S, Alfar E, Aljani B, Alves TC, Amiel S, Andoniadou CL, Bandral M, Belavgeni A, Berger I, Birkenfeld A, Bonifacio E, Chavakis T, Chawla P, Choudhary P, Cujba AM, Delgadillo Silva LF, Demcollari T, Drotar DM, Duin S, El-Agroudy NN, El-Armouche A, Eugster A, Gado M, Gavalas A, Gelinsky M, Guirgus M, Hansen S, Hanton E, Hasse M, Henneicke H, Heller C, Hempel H, Hogstrand C, Hopkins D, Jarc L, Jones PM, Kamel M, Kämmerer S, King AJF, Kurzbach A, Lambert C, Latunde-Dada Y, Lieberam I, Liers J, Li JW, Linkermann A, Locke S, Ludwig B, Manea T, Maremonti F, Marinicova Z, McGowan BM, Mickunas M, Mingrone G, Mohanraj K, Morawietz H, Ninov N, Peakman M, Persaud SJ, Pietzsch J, Cachorro E, Pullen TJ, Pyrina I, Rubino F, Santambrogio A, Schepp F, Schlinkert P, Scriba LD, Siow R, Solimena M, Spagnoli FM, Speier S, Stavridou A, Steenblock C, Strano A, Taylor P, Tiepner A, Tonnus W, Tree T, Watt F, Werdermann M, Wilson M, Yusuf N, Ziegler CG. The transCampus Metabolic Training Programme Explores the Link of SARS-CoV-2 Virus to Metabolic Disease. Horm Metab Res., 2021;53(3):204-206. doi: 10.1055/a-1377-6583.
Siragusa M, Oliveira Justo AF, Malacarne PF, Strano A, Buch A, Withers B, Peters KG, Fleming I. VE-PTP inhibition elicits eNOS phosphorylation to blunt endothelial dysfunction and hypertension in diabetes. Cardiovasc Res. 2021;117(6):1546-1556. doi: 10.1093/cvr/cvaa213.
2020
Li W, Stauske M, Luo X, Wagner S, Vollrath M, Mehnert CS, Schubert M, Cyganek L, Chen S, Hasheminasab SM, Wulf G, El-Armouche A, Maier LS, Hasenfuss G, Guan K. Disease Phenotypes and Mechanisms of iPSC-Derived Cardiomyocytes From Brugada Syndrome Patients With a Loss-of-Function SCN5A Mutation. Front Cell Dev Biol., 2020;8, 1181. doi: 10.3389/fcell.2020.592893.
Luo X, Li W, Künzel K, Henze S, Cyganek L, Strano A, Poetsch MS, Schubert M, Guan K, IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency. Front Cell Dev Biol., 2020;8, 772. doi: 10.3389/fcell.2020.00772.
Poetsch MS, Strano A, Guan K. Role of Leptin in Cardiovascular Diseases. Front Endocrinol (Lausanne). 2020;11:354. doi: 10.3389/fendo.2020.00354.
Grunert M, Appelt S, Schönhals S, Mika K, Cui H, Cooper A, Cyganek L, Guan K, Rickert-Sperling S. Induced pluripotent stem cells of patients with Tetralogy of Fallot reveal transcriptional alterations in cardiomyocyte differentiation. Sci Rep, 2020;10:10921. doi: 10.1038/s41598-020-67872-z.
Knottnerus SJG, Mengarelli I, Wüst RCI, Baartscheer A, Bleeker JC, Coronel R, Ferdinandusse S, Guan K, IJlst L, Li W, Luo X, Portero VM, Ulbricht Y, Visser G, Wanders RJA, Wijburg FA, Verkerk AO, Houtkooper RH, Bezzina CR. Electrophysiological abnormalities in VLCAD deficient hiPSC-cardiomyocytes can be improved by lowering accumulation of fatty acid oxidation intermediates. Int J Mol Sci, 2020;21(7):2589. pii: E5105. doi: 10.3390/ijms21072589.
Ruiz-Velasco A, Zi M, Hille SS, Azam T, Kaur N, Jiang J, Nguyen B, Sekeres K, Binder P, Collins L, Pu F, Xiao H, Guan K, Frey N, Cartwright EJ, Müller OJ, Wang X, Liu W. Targeting mir128-3p alleviates myocardial insulin resistance and prevents ischemia-induced heart failure. Elife, 2020;9:e54298. doi: 10.7554/eLife.54298.
Schubert M, Hansen S, Leefmann J, Guan K. Repurposing Antidiabetic Drugs for Cardiovascular Disease. Front Physiol. 2020;11, 1168. doi: 10.3389/fphys.2020.568632.
Li W, Luo X, Ulbricht Y, Wagner M, Piorkowski C, El-Armouche A, Guan K. Establishment of an automated patch-clamp platform for electrophysiological and pharmacological evaluation of hiPSC-CMs. Stem Cell Res, 2019;41:101662. doi: 10.1016/j.scr.2019.101662
Kolanowski TJ*, Busek M*, Schubert M*, Dmitrieva A, Binnewerg B, Pöche J, Fisher K, Schmieder F, Grünzner S, Hansen S, Richter A, El-Armouche A, Sonntag F, Guan K. Enhanced structural maturation of human induced pluripotent stem cell-derived cardiomyocytes under a controlled microenvironment in a microfluidic system. Acta Biomater. 2020;102, 273–286. doi: 10.1016/j.actbio.2019.11.044.
Busek M, Schubert M, Guan K, Sonntag F, Schmieder F, Marschner U, Richter A. Microphysiological system for heart tissue - going from 2D to 3D culture. Curr Direct Biomedical Eng, 2019;5:269-272. doi: 10.1515/cdbme-2019-0068.
Hoffmann M, Kant TA, Emig R, Rausch JSE, Newe M, Schubert M, Künzel K, Winter, L, Klapproth E, Peyronnet R, Ravens U, El-Armouche A, Künzel SR. Repurposing mesalazine against cardiac fibrosis in vitro. Naunyn-Schmiedeberg’s Arch Pharmacol. 2020;394, 533-543. doi: 10.1007/s00210-020-01998-9.
Volland C, Schott P, Didié M, Männer J, Unsöld B, Toischer K, Schmidt C, Urlaub H, Nickels K, Knöll R, Schmidt A, Guan K, Hasenfuß G, Seidler T. Control of p21Cip by BRCA1-associated protein is critical for cardiomyocyte cell cycle progression and survival. Cardiovasc Res. 2020;116(3):592-604. doi: 10.1093/cvr/cvz177.
Pötsch MS, Ishida J, Palus S, Tschirner A, von Haehling S, Doehner W, Anker SD, Springer J. MT-102 prevents tissue wasting and improves survival in a rat model of severe cancer cachexia. J Cachexia Sarcopenia Muscle. 2020;11(2):594-605. doi: 10.1002/jcsm.12537.
Siragusa M, Oliveira Justo AF, Malacarne PF, Strano A, Buch A, Withers B, Peters KG, Fleming I. VE-PTP inhibition elicits eNOS phosphorylation to blunt endothelial dysfunction and hypertension in diabetes. Cardiovasc Res. 2021;117(6):1546-1556. doi: 10.1093/cvr/cvaa213.
2019
Schubert M*, Binnewerg B*, Voronkina A, Muzychka L, Wysokowski M, Petrenko I, Kovalchuk V, Tsurkan M, Martinovic R, Bechmann N, Ivanenko VN, Fursov A, Smo-lii OB, Fromont J, Joseph Y, Bornstein SR, Giovine M, Erpenbeck D, Guan K, Ehrlich H. Naturally Prefabricated Marine Biomaterials: Isolation and Applications of Flat Chitinous 3D Scaffolds from Ianthella labyrinthus (Demospongiae: Verongiida). Int J Mol Sci. 2019;20, 5105. doi: 10.3390/ijms20205105.
Binder P, Wang S, Radu M, Zin M, Collins L, Khan S, Li Y, Sekeres K, Humphreys N, Swanton E, Reid A, Pu F, Oceandy D, Guan K, Hille SS, Frey N, Müller OJ, Cartwright EJ, Chernoff J, Wang X, Liu W. Pak2 as a Novel Therapeutic Target for Cardioprotective Endoplasmic Reticulum Stress Response. Circ Res. 2019; 124(5):696-711. doi: 10.1161/CIRCRESAHA.118.312829.
Binnewerg B*, Schubert M*, Voronkina A, Muzychka L, Wysokowski M, Petrenko I, Djurović M, Kovalchuk V, Tsurkan M, Martinovic R, Bechmann N, Fursov A, Ivanenko VN, Tabachnick KR, Smolii OB, Joseph Y, Giovine M, Bornstein SR, Stelling AL, Tunger A, Schmitz M, Taniya OS, Kovalev IS, Zyryanov GV, Guan K, Ehrlich H, Marine biomaterials: Biomimetic and pharmacological potential of cultivated Aplysina aerophoba marine demosponge. Materials Science and Engineering. 2019;109, 110566. doi: 10.1016/j.msec.2019.110566.
Kovalchuk V, Voronkina A, Binnewerg B, Schubert M, Muzychka L, Wysokowski M, Tsurkan MV, Bechmann N, Petrenko I, Fursov A, Martinovic R, Ivanenko VN, Fromont J, Smolii OB, Joseph Y, Giovine M, Erpenbeck D, Gelinsky M, Springer A, Guan K, Bornstein SR, Ehrlich H. Naturally Drug-Loaded Chitin: Isolation and Applications. Mar Drugs. 2019;17, 574. doi: 10.3390/md17100574.
Machałowski T, Wysokowski M, Żółtowska-Aksamitowska S, Bechmann N, Binnewerg B, Schubert M, Guan K, Bornstein SR, Czaczyk K, Pokrovsky O, Kraft M, Bertau M, Schimpf C, Rafaja D, Tsurkan M, Galli R, Meissner H, Petrenko I, Fursov A, Voronkina A, Figlerowicz M, Joseph Y, Jesionowski T, Ehrlich H. Spider Chitin. The biomimetic potential and applications of Caribena versicolor tubular chitin. Carbohydr Polym. 2019;226, 115301. doi: 10.1016/j.carbpol.2019.115301.
Machałowski T, Wysokowski M, Tsurkan MV, Galli R, Schimpf C, Rafaja D, Brendler E, Viehweger C, Żółtowska-Aksamitowska S, Petrenko I, Czaczyk K, Kraft M, Bertau M, Bechmann N, Guan K, Bornstein SR, Voronkina A, Fursov A, Bejger M, Biniek-Antosiak K, Rypniewski W, Figlerowicz M, Pokrovsky O, Jesionowski T, Ehrlich H. Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle. Molecules. 2019;16;24(20):3736. doi: 10.3390/molecules24203736.
Veerman CC, Mengarelli I, Koopman CD, Wilders R, van Amersfoorth SC, Bakker D, Wolswinkel R, Hababa M, de Boer TP, Guan K, Milnes J, Lodder EM, Bakkers J, Verkerk AO, Bezzina CR. Genetic variation in GNB5 causes bradycardia by augmenting the cholinergic response via increased acetylcholine-activated potassium current (IK,ACh). Dis Model Mech. 2019;12(7):dmm037994. doi: 10.1242/dmm.037994.
Casini S, Marchal GA, Kawasaki M, Nariswari FA, Portero VM, Guan K, Driessen AHG, Veldkamp MW, Mengarelli I, de Groot JR, Verkerk AO, Remme CA. Absence of functional Nav1.8 channels in non-diseased atrial and ventricular cardiomyocytes. Cardiovasc Drugs Ther, 2019;33(6):649-660. doi: 10.1007/s10557-019-06925-6.
2018
Cyganek L, Tiburcy M, Sekeres K, Gerstenberg K, Bohnenberger H, Lenz C, Henze S, Stauske M, Salinas G, Zimmermann WH, Hasenfuss G, Guan K. Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes. JCI Insight. 2018 Jun 21;3(12):e99941. doi: 10.1172/jci.insight.99941.
Singh S, Lämmle S, Giese H, Kämmerer S, Meyer-Roxlau S, Alfar EA, Dihazi H, Guan K, El-Armouche A, Richter F. The reduced activity of PP-1α under redox stress condition is a consequence of GSH-mediated transient disulfide formation. Sci Rep, 2018;8:17711. doi: 10.1038/s41598-018-36267-6.
Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, Bernhard O, Balleiniger M, Alfar EA, Zieseniss A, Toischer K, Guan K, Rizzoli SO, Brügger B, Fischer A, Katschinski DM, Rehling P, Dudek J. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia. Cell Rep, 2018;25:561-570.e6. doi: 10.1016/j.celrep.2018.09.057.
Mohamed BA, Hartmann N, Tirilomis P, Sekeres K, Li W, Neef S, Richter C, Zeisberg EM, Kattner L, Didié M, Guan K, Schmitto JD, Lehnart SE, Luther S, Voigt N, Seidler T, Sossalla S, Hasenfuss G, Toischer K. Sarcoplasmic reticulum calcium leak contributes to arrhythmia but not to heart failure progression. Sci Transl Med, 2018;10:eaan0724. doi: 10.1126/scitranslmed.aan0724.
Ben Jehuda R, Eisen B, Shemer Y, Mekies LN, Reiter I, Guan K, Cui H, Haron-Khun S, Sperling SR, Gherghiceanu M, Arad M, Binah O. CRISPR correction of the PRKAG2 gene mutation in the patient's iPSC-derived cardiomyocytes eliminates the electrophysiological and structural abnormalities. Heart Rhythm, 2018;1:267-276. doi: 10.1016/j.hrthm.2017.09.024.
2017 und früher
Streckfuß-Bömeke K, Tiburcy M, Fomin A, Luo X, Li W, Fischer C, Özcelik C, Perrot A, Sossalla S, Haas J, Vidal RO, Rebs S, Khadjeh, S, Meder B, Bonn S, Linke WA, Zimmermann W-H, Hasenfuß G, Guan K. Severe DCM phenotype of patient harboring RBM20 mutation S635A can be modeled by patient-specific induced pluripotent stem cell-derived cardiomyocytes. J Mol Cell Cardiol. 2017;113:9-21. doi: 10.1016/j.yjmcc.2017.09.008.
Busek M, Kolanowski T, Grünzner S, Steinfelder C, Guan K*, Sonntag F*. Microfluidic system for enhanced cardiac tissue formation. Curr Direct Biomedical Eng, 2017;3:367-370. doi: 10.1515/cdbme-2017-0076. doi: 10.1515/cdbme-2017-0076.
Weber S, Zeller M, Guan K, Wunder F, Wagner M, El-Armouche A. PDE2 at the crossway between cAMP and cGMP signalling in the heart. Cell Signal, 2017;38:76-84. doi: 10.1016/j.cellsig.2017.06.020.
Borchert T, Hübscher D, Guessoum CI, Lam TD, Ghadri JR, Schellinger IN, Tiburcy M, Liaw NY, Li Y, Haas J, Sossalla S, Huber MA, Cyganek L, Jacobshagen C, Dressel R, Raaz U, Nikolaev VO, Guan K, Thiele H, Meder B, Wollnik B, Zimmermann WH, Luscher TF, Hasenfuss G, Templin C and Streckfuss-Bömeke K. Catecholamine-Dependent beta-Adrenergic Signaling in a Pluripotent Stem Cell Model of Takotsubo Cardiomyopathy. J Am Coll Cardiol. 2017;70:975-991. doi: 10.1016/j.jacc.2017.06.061.
Chowdhury SK, Liu W, Zi M, Li Y, Wang S, Tsui H, Prehar S, Castro S, Zhang H, Ji Y, Zhang X, Xiao R, Zhang R, Lei M, Cyganek L, Guan K, Millar CB, Liao X, Jain MK, Boyett MR, Cartwright EJ, Shiels HA and Wang X. Stress-Activated Kinase Mitogen-Activated Kinase Kinase-7 Governs Epigenetics of Cardiac Repolarization for Arrhythmia Prevention. Circulation. 2017;135:683-699. doi: 10.1161/CIRCULATIONAHA.116.022941.
Gröschel C*, Hübscher D*, Nolte J, Monecke S, Sasse A, Elsner L, Paulus W, Trenkwalder C, Polic B, Mansouri A, Guan K*and Dressel R*. Efficient Killing of Murine Pluripotent Stem Cells by Natural Killer (NK) Cells Requires Activation by Cytokines and Partly Depends on the Activating NK Receptor NKG2D. Front Immunol. 2017;8:870. doi: 10.3389/fimmu.2017.00870.
Meyer-Roxlau S, Lämmle S, Opitz A, Künzel S, Joos JP, Neef S, Sekeres K, Sossalla ST, Schöndube F, Alexiou K, Maier LS, Dobrev D, Guan K, Weber S, El-Armouche A. Differential regulation of protein phosphatase 1 (PP1) isoforms in human heart failure and atrial fibrillation. Basic Res Cardiol, 2017;112:43. doi: 10.1007/s00395-017-0635-0.
Kolanowski TJ, Antos CL and Guan K. Making human cardiomyocytes up to date: Derivation, maturation state and perspectives. Int J Cardiol. 2017;241:379-386. doi: 10.1016/j.ijcard.2017.03.099.
Hübscher D, Kaiser D, Elsner L, Monecke S, Dressel R and Guan K. The Tumorigenicity of Multipotent Adult Germline Stem Cells Transplanted into the Heart Is Affected by Natural Killer Cells and by Cyclosporine A Independent of Its Immunosuppressive Effects. Front Immunol. 2017;8:67. doi: 10.3389/fimmu.2017.00067.
Veerman CC, Mengarelli I, Lodder EM, Kosmidis G, Bellin M, Zhang M, Dittmann S, Guan K, Wilde AAM, Schulze‐Bahr E, Greber B, Bezzina CR*, Verkerk AO*. Switch from fetal to adult SCN5A isoform in human induced pluripotent stem cell–derived cardiomyocytes unmasks the cellular phenotype of a conduction disease–causing Mutation. J Am Heart Assoc, 2017;6:e005135, doi: 10.1161/JAHA.116.005135.
Dudek J, Cheng IF, Chowdhury A, Wozny K, Balleininger M, Reinhold R, Grunau S, Callegari S, Toischer K, Wanders RJ, Hasenfuss G, Brügger B, Guan K and Rehling P. Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome. EMBO Mol Med. 2016;8:139-54. doi: 10.15252/emmm.201505644.
Liu X, Qi J, Xu X, Zeisberg M, Guan K and Zeisberg EM. Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method. Differentiation. 2016;92:225-236. doi: 10.1016/j.diff.2016.05.004.
Veerman CC, Mengarelli I, Guan K, Stauske M, Barc J, Tan HL, Wilde AA, Verkerk AO and Bezzina CR. hiPSC-derived cardiomyocytes from Brugada Syndrome patients without identified mutations do not exhibit clear cellular electrophysiological abnormalities. Sci Rep. 2016;6:30967. doi: 10.1038/srep30967.
Chuykin I., Stauske M., Guan K. Chapter 9 Spermatogonial Stem Cells. In: Gustav Steinhoff (Hg, 2nd ed.) Regenerative Medicine: From Protocol to Patient. Springer Verlag, London, 2016;219-249.
Chuykin I, Stauske M, Guan K. Chapter 4 Spermatogonial Stem Cells. In: Gustav Steinhoff (Hg, 3nd ed.) Regenerative Medicine - From Protocol to Patient: 2. Stem cell Science and Technology. Springer Verlag, Switzerland, 2016;77-110.
Chang Liao M-L, Mutoh H, de Boer T, Raad N, Richter C, Wagner E, Downie BR, Unsöld B, Arooj I, Streckfuss-Bömeke K, Doeker S, Luther S, Guan K, Wagner S, Lehnart S, Maier LS, Stühmer W, Wettwer E, van Veen T, Morlock MM, Knöpfel T, Zimmermann WH. Sensing cardiac electrical activity with a cardiomyocyte targeted optogenetic voltage indicator. Circ Res, 2015;117:401-412. doi: 10.1161/CIRCRESAHA.117.306143.
Kruse V, Hamann C, Hübscher D, Cyganek L, Streckfuss-Bömeke K, Monecke S, Guan K*, Dressel R*. Human induced pluripotent stem cells are targets for allogeneic and autologous natural killer cells. PLoS One, 2015;10(5):e0125544. doi: 10.1371/journal.pone.0125544.
van Putten RMEM, Mengarelli I, Guan K, Zegers JG, van Ginneken ACG, Verkerk AO, Wilders R. Ion channelopathies in human induced pluripotent stem cell derived cardiomyocytes: a dynamic clamp study with virtual IK1. Front Physiol, 2015;6:7. doi: 10.3389/fphys.2015.00007.
Streckfuss-Bömeke K, Jende J, Cheng I-F, Hasenfuss G, Guan K. Efficient generation of hepatic cells from multipotent adult mouse germline stem cells using an OP9 co-culture system. Cell Reprogram, 2014;16:65-76. doi: 10.1089/cell.2013.0057.
Cyganek L, Chen S, Borchert T, Guan K. Cardiac progenitor cells and their therapeutic application for cardiac repair. J Clin Exp Cardiol, 2013;S11:008.
Dudek J, Cheng I-F, Balleininger M, Vaz FM, Streckfuss-Bömeke K, Hübscher D, Vukotic M, Wanders RJA, Rehling P, Guan K. Cardiolipin deficiency affects respiratory chain function and organization in an induced pluripotent stem cell model of Barth syndrome. Stem Cell Res, 2013;11:806-819. doi: 10.1016/j.scr.2013.05.005.
Streckfuss-Bömeke K, Wolf F, Azizian A, Stauske M, Tiburcy M, Wagner S, Hübscher D, Dressel R, Chen S, Jende J, Wulf G, Lorenz V, Schön MP, Maier LS, Zimmermann WH, Hasenfuss G, Guan K. Comparative study of human-induced pluripotent stem cells derived from bone marrow cells, hair keratinocytes, and skin fibroblasts. Eur Heart J, 2013;34:2618-2629. doi: 10.1093/eurheartj/ehs203.
Guan K, Hasenfuss G. Cardiac resident progenitor cells: evidence and functional significance. Eur Heart J, 2013;34:2784-2787. doi: 10.1093/eurheartj/ehs208.
Chuykin I, Schulz H, Guan K, Bader M. PTHRP/Adenylate cyclase promotes parietal whereas Wnt/beta-catenin signaling promotes visceral endoderm differentiation of rat XEN cells. J Cell Sci, 2013;126:128-138. doi: 10.1242/jcs.110239.
Unsöld B, Schotola H, Jacobshagen C, Seidler T, Sossalla S, Emons J, Klede S, Knöll R, Guan K, El-Armouche A, Linke WA, Kögler H, Hasenfuss G. Age-dependent changes in contractile function and passive elastic properties of myocardium from mice lacking muscle LIM protein (MLP). Eur J Heart Fail, 2012;14:430-437. doi: 10.1093/eurjhf/hfs020.
Cheng I-F, Kaiser D, Hübscher D, Hasenfuss G, Guan K*, Schaefer K*. Differentiation of multipotent adult germline stem cells derived from mouse testis into functional endothelial cells. J Vasc Res, 2012;49:207-220. (*shared correspondence author). doi: 10.1159/000332910.
Guan K, Cheng I-F, Baazm M. Human spermatogonial stem cells: a novel therapeutic hope for heart regeneration and repair? Future Cardiol, 2012;8:39-51. doi: 10.2217/fca.11.78.
Dressel R, Nolte J, Elsner L, Novota P, Guan K, Streckfuss-Bömeke K, Hasenfuss G, Jaenisch R, Engel W. Pluripotent stem cells are highly susceptible targets for syngeneic, allogeneic, and xenogeneic natural killer cells. FASEB J, 2010;24:2164-2177. doi: 10.1096/fj.09-134957.
Ciraolo E, Morello F, Hobbs RM, Wolf F, Marone R, Iezzi M, Lu X, Mengozzi G, Altruda F, Sorba G, Guan K, Wymann MP, Hirsch E. Essential role of the p110β subunit of phosphoinositide 3-OH kinase in male fertility. Mol Biol Cell, 2010;21:704-711. doi: 10.1091/mbc.e09-08-0744.
Dressel R*, Guan K*, Nolte J, Elsner L, Monecke S, Nayernia K, Hasenfuss G, Engel W. Multipotent adult germ-line stem cells, like other pluripotent stem cells, can be killed by cytotoxic T lymphocytes despite low expression of major histocompatibility complex class I molecules. Biol Direct, 2009;4:31. (*shared first authorship). doi: 10.1186/1745-6150-4-31.
Streckfuss-Bömeke K, Vlasov A, Yin D, Hülsmann S, Nayernia K, Engel W, Hasenfuss G, Guan K. Generation of functional neurons and glia from multipotent adult mouse germline stem cells. Stem Cell Res, 2009;2:139-154. doi: 10.1016/j.scr.2008.09.001.
Guan K*, Wolf F, Becker A, Engel W, Nayernia K, Hasenfuss G. Isolation and cultivation of stem cells from adult mouse testes. Nat Protoc, 2009;4:143-154. (*correspondence author). doi: 10.1038/nprot.2008.242.
Mardanpour P, Guan K, Glaeser T, Lee JH, Nolte J, Wulf G, Hasenfuss G, Engel W, Brüstle O, Nayernia K. Reprogramming male germ cells to pluripotent stem cells. In: Baharvand H (ed.) Trends in Stem Cell Biology and Technology. Humana Press Inc., Totowa, New Jersey, 2009;71-81.
Mardanpour P, Guan K, Nolte J, Lee JH, Hasenfuss G, Engel W, Nayernia K. Potency of germ cells and its relevance for regenerative medicine. J Anat, 2008;213:26-29. doi: 10.1111/j.1469-7580.2008.00930.x.
Zovoilis A, Nolte J, Drusenheimer N, Zechner U, Hada H, Guan K, Hasenfuss G, Nayernia K, Engel W. Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles. Mol Hum Reprod, 2008;14:521-529. doi: 10.1093/molehr/gan044.
Guan K*, Wagner S*, Unsöld B, Maier LS, Kaiser D, Hemmerlein B, Nayernia K, Engel W, Hasenfuss G. Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells. Circ Res, 2007;100:1615-1625. (*shared first authorship). doi: 10.1161/01.RES.0000269182.22798.d9.
Guan K, Hasenfuss G. Do stem cells in the heart truly differentiate into cardiomyocytes? J Mol Cell Cardiol, 2007; 43:377-87. doi: 10.1016/j.yjmcc.2007.07.056.
Nayernia K, Lee JH, Engel W, Nolte J, Drusenheimer N, Rathsack K, Dev A, Wulf G, Ehrmann IE, Elliott D, Zechner U, Haaf T, Meinhardt A, Michelmann HW, Hasenfuss G, Guan K. From stem cells to germ cells and from germ cells to stem cells. Iran J Reprod Med, 2007;5:41-44.
Guan K, Hasenfuss G. The prospect of adult spermatogonial stem cells for therapeutic cardiac regeneration. In: L.J. Field and K.C. Wollert (eds.) Rebuilding the Infarcted Heart. Informa Healthcare, 2007;3-24.
Guan K, Nayernia K, Maier L, Wagner S, Dressel R, Lee JH, Nolte J, Wolf F, Li M, Engel W, Hasenfuss G. Pluripotency of spermatogonial stem cells from adult mouse testis. Nature, 2006;440:1199-1203. (*shared first authorship). doi: 10.1038/nature04697.
Czyz J, Guan K, Zeng Q, Wobus AM. Loss of b1 integrin function results in upregulation of connexin expression in embryonic stem cell-derived cardiomyocytes. Int J Dev Biol, 2005;49:33-41. doi: 10.1387/ijdb.041835jc.
Czyz J, Guan K, Zeng Q, Nikolova T, Meister A, Schönborn F, Schuderer J, Kuster N, Wobus AM. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics, 2004;25:296-307. doi: 10.1002/bem.10199.
Höcht-Zeisberg E, Kahnert H, Guan K, Wulf G, Hemmerlein B, Schlott T, Tenderich G, Körfer R, Raute-Kreinsen U, Hasenfuss G. Cellular repopulation of myocardial infarction in patients with sex-mismatched heart transplantation. Eur Heart J, 2004;25:749-758. doi: 10.1016/j.ehj.2004.01.017.
Wobus AM, Guan K, Yang HT, Boheler KR. Embryonic stem cells as a model to study cardiac, skeletal muscle and vascular smooth muscle cell differentiation. Methods Mol Biol, 2002;185:127-156. doi: 10.1385/1-59259-241-4:127.
Guan K, Chang H, Rolletschek A, Wobus AM. Embryonic stem cell-derived neurogenesis: Retinoic acid induction and lineage selection of neuronal cells. Cell Tissue Res, 2001;305:171-176. doi: 10.1007/s004410100416.
Wobus AM, Guan K, Pich U. In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes. (2001) Methods Mol Biol, 185:263-286. doi: 10.1385/1-59259-220-1:263.
Rohwedel J, Guan K, Hegart C, Wobus AM. Embryonic stem cells as an in vitro model for mutagenicity, cytotoxicity and embryotoxicity studies: Present state and future prospects. Toxicol In Vitro, 2001;15:741-753. doi: 10.1016/S0887-2333(01)00074-1.
Hegert C, Kramer J, Hargus G, Müller J, Guan K, Wobus AM, Müller PK, Rohwedel J. Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells. J Cell Sci, 2002;115:4617-4628. doi: 10.1242/jcs.00171.
Gottwald E, Wobus AM, Guan K, Sontag W, Weibezahn KF, Dertinger H. Interferential electric field treatment revealed a low increase of spontaneous cardiac differentiation but no cyclic cardiac-specific gene expression in pluripotent embryonal carcinoma P19 cells. Electromagn Biol Med, 2002;21: 05-118. doi: 10.1081/JBC-120006783.
Rolletschek A, Chang H, Guan K, Czyz J, Meyer M, Wobus AM. Differentiation of embryonic stem cell-derived dopaminergic neurons is enhanced by survival-promoting factors. Mech Develop, 2001;105:93-104. doi: 10.1016/s0925-4773(01)00385-9.
Schmidt MM, Guan K, Wobus AM. Lithium effects lineage-specifically differentiation patterns and gene expression levels of mouse embryonic stem (ES) cells in vitro. Int J Dev Biol, 2001;45:421-429.
Guan K, Czyz J, Fürst DO, Wobus AM. Expression and cellular distribution of av integrins in b1 integrin-deficient embryonic stem cell-derived cardiac cells. J Mol Cell Cardiol, 2001;33:521-532. doi:10.1006/JMCC.2000.1326.
Vassilieva S, Guan K, Pich U, Wobus AM. Establishment of SSEA-1 and Oct-4 expressing rat ES-like cell lines and effects of cytokines of the IL-6 family on clonal growth. Exp Cell Res, 2000;258:361-373. doi: 10.1006/excr.2000.4940.
Kramer J, Hegert C, Guan K, Wobus AM, Müller PK, Rohwedel J. Embryonic stem cell-derived chondrogenic differentiation in vitro: activation by BMP-2 and BMP-4. Mech Develop, 2000;92:193-205. doi: 10.1016/s0925-4773(99)00339-1.
Guan K, Fürst DO, Wobus AM. Modulation of sarcomere organization during embryonic stem cell-derived cardiomyocyte differentiation. Eur J Cell Biol, 1999;78:813-823. doi: 10.1016/S0171-9335(99)80032-6.
Guan K, Rohwedel J, Wobus AM. Embryonic stem cell differentiation models: cardiogenesis, myogenesis, neurogenesis, epithelial and vascular smooth muscle cell differentiation in vitro. Cytotechnology, 1999;30:211-226. doi: 10.1023/A:1008041420166.
Guan K, Schmidt MM, Ding Q, Chang H, Wobus AM. Embryonic stem cells in vitro - prospects for cell and developmental biology, embryotoxicology and cell therapy. ALTEX-Altern Anim Ex, 1999;16:135-141.
Rohwedel J, Guan K, Wobus AM. Induction of cellular differentiation by retinoic acid in vitro. Cells Tissues Organs, 1999;165:190-202. doi: 10.1159/000016699.
Rohwedel J, Guan K, Zuschratter W, Jin S, Ahnert-Hilger G, Fürst DO, Fässler R, Wobus AM. Loss of b1 integrin function results in a retardation of myogenic, but an acceleration of neuronal differentiation of embryonic stem cells in vitro. Dev Biol, 1998;201:167-184 (*shared first authorship). doi: 10.1006/dbio.1998.9002.
Wobus AM, Guan K. Embryonic stem cell-derived cardiac differentiation: Modulation of differentiation and "loss-of-function" analysis in vitro. Trends Cardiovas Med, 1998;8:64-74. doi: 10.1016/S1050-1738(97)00129-1.
Rohwedel J, Kleppisch T, Pich U, Guan K, Jin S, Zuschratter W, Hopf C, Hoch W, Hescheler J, Witzemann V, Wobus AM. Formation of postsynaptic-like membranes during differentiation of embryonic stem cells in vitro. Exp Cell Res, 1998;239:214-225. doi: 10.1006/excr.1997.3903.
Wobus AM, Guan K, Shan J, Wellner MC, Rohwedel J, Ji Guanju, Fleischmann B, Katus HA, Hescheler J, Franz WM. Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes. J Mol Cell Cardiol, 1997;29:1525-1539. doi: 10.1006/jmcc.1997.0433.
Fässler R, Rohwedel J, Maltsev V, Bloch W, Lentini S, Guan K, Gullberg D, Hescheler J, Addicks K, Wobus AM. Differentiation and integrity of cardiac muscle cells are impaired in the absence of b1 integrin. J Cell Sci, 1996;109:2989-2999. doi: 10.1242/jcs.109.13.2989.