Nicole Poulsen

2023

Diatom adhesive trail proteins acquired by horizontal gene transfer from bacteria serve as primers for marine biofilm formation
J. Zackova Suchanova, G. Bilcke, B. Romanowska, A. Fatlawi, M. Pippel, A. Skeffington, M. Schroeder, W. Vyverman, K. Vandepoele, N. Kröger, N. Poulsen
New Phytologist 240, 770-783 (2023)

Thalassiosira pseudonana (Cyclotella nana) (Hustedt) Hasle et Heimdal (Bacillariophyceae): A genetically tractable model organism for studying diatom biology, including biological silica formation
N. Poulsen, N. Kröger
J. Phycol. early view (2023)

On the role of cell surface associated, mucin-like glycoproteins in the pennate diatom Craspedostauros australis (Bacillariophyceae)*
N. Poulsen, H. Hennig, V. F. Geyer, S. Diez, R. Wetherbee, S. Fitz-Gibbon, M. Pellegrini, N. Kröger
J. Phycol. 59, 54-69 (2023)

2022

Shedding light on silica biomineralization by comparative analysis of the silica-associated proteomes from three diatom species
Skeffington, A*., Gentzel, M., Ohara, A., Milentyev, A., Heintze, C. Böttcher, L., Görlich, S. Shevchenko, A., Poulsen, N., Kröger, N*.
Plant J. 110, 1700-1716 (2022).

© Springer

Chapter 14: Adhesion and motility
Poulsen, N., Davutoglu, M.G., Zackova Suchanova, J.
The Molecular Life of Diatoms, (eds Falciatore, A., Mock, T.) Springer (in press)

2021

Diurnal transcript profiling of the diatom Seminavis robusta reveals adaptations to a benthic lifestyle
Bilcke, G., Osuna-Cruz, C.M., Santana Silva, M., Poulsen, N., D’hondt, S., Bulankova, P., Vyverman, W., De Veylder, L. and Vandepoele, K.
The Plant Journal (2021)107,315–336

2020

Chitin synthase localization in the diatom Thalassiosira pseudonana
M. Wustman, N. Poulsen, N. Kröger, K. H. van Pée
BMC Materials 2, Article number: 10, 2020.

The Seminavis robusta genome provides insights into the evolutionary adaptations of benthic diatoms
C. Osuna-Cruz et al.
Nature Communications, 11, Article: 3320, 2020.

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta
G. Bilcke, K. Van den Berge, S. De Decker, E. Bonneure, N. Poulsen, P. Bulankova, C. M. Osuna-Cruz, J. Dickenson, K. Sabbe, G. Pohnert, K. Vandepoele, S. Mangelinckx, L. Clement, L. De Veylder, W. Vyverman
ISME Journal (2020)

Genetically Programmed Regioselective Immobilization of Enzymes in Biosilica Microparticles
E. Kumari, S. Görlich, N. Poulsen, N. Kröger
Adv. Funct. Mater., 30, 2000442, 2020.

2019

Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis
M. Lachnit, M.T. Buhmann, J. Klemm, N. Kröger, N. Poulsen*
Phil. Trans. R. Soc. B., 374: 20190196, 2019.

2018

Biomineralization as a paradigm of directional solidification: A physical model for molluscan shell ultrastructural morphogenesis
V. Schoeppler, L. Gránásy, E. Reich, N. Poulsen, R. de Kloe, P. Cook, A. Rack, T. Pusztai, I. Zlotnikov*
Adv. Mater. 30 , 1803855, 2018.

2016

Establishing super-resolution imaging for proteins in diatom biosilica
P. Gröger, N. Poulsen, J. Klemm, N. Kröger, M. Schlierf
Scientific Reports 6, Article number: 36824, 2016.

Biochemical composition and assembly of biosilica-associated insoluble organic matrices from the diatom Thalassiosira pseudonana
A. Kotzsch, D. Pawolski, A. Milentyev, Anna Shevchenko, A. Scheffel, N. Poulsen, Andrej Shevchenko, N. Kröger
J. Biol. Chem. 291, 4982-4997, 2016.

2014

A Tyrosine-rich Cell Surface Protein in the Diatom Amphora coffeaeformis identified through Transcriptome Analysis and Genetic Transformation
M. T. Buhmann, N. Poulsen, J. Klemm, M. R. Kennedy, C. D. Sherrill, N. Kröger
PLOS ONE 9, e110369, 2014.

Isolation and biochemical characterization of underwater adhesives from diatoms
N. Poulsen*, N. Kröger , M. J. Harrington, E. Brunner, S. Paasch and M. T. Buhmann
Biofouling 30, 513-523, 2014.

2013

Nitrate and carbonate concentration for high glucose content in microalgae
N. Kröger, S. J. Miller, N. Poulsen
US patent US8569040 B1 (approved).

Pentalysine clusters mediate silica targeting of silaffins in Thalassiosira pseudonana
N. Poulsen, A. Scheffel, V. C. Sheppard, P. M. Chesley, N. Kröger
J. Biol Chem. 288, 20100-20109, 2013.

2012

Live Diatom Silica Immobilization of Multimeric and Redox-active Enzymes
V. C. Sheppard, A. Scheffel, N. Poulsen, N. Kröger
Appl. Environ. Microbiol. 78, 211-218, 2012.

2011

Nanopatterned protein microrings from a diatom that direct silica morphogenesis
A. Scheffel, N. Poulsen, S. Shian, N. Kröger
Proc. Natl. Acad. Sci. USA 108, 3175-3180, 2011.

A Systematic Screen to Discover and Analyze Apicoplast Proteins Identifies a Conserved and Essential Protein Import Factor
L. Sheiner, J. Piester, N. Poulsen, W. Beatty, M. Behnke, O. Lucas, M. W. White, B. Striepen
PLOS Pathogens 2011 Dec;7(12):e1002392, 2011

2010

Characterization of an endoplasmic reticulum-associated silaffin kinase from the diatom Thalassiosira pseudonana
V. C. Sheppard, N. Poulsen, N. Kröger
J. Biol. Chem. 285, 1166-1176, 2010.

2009

Protein-Mediated Layer-by-Layer Syntheses of Freestanding Microscale Titania Structures with Biologically Assembled 3-D Morphologies
Y. Fang, Q. Wu, M. B. Dickerson, Y. Cai, S. Shian, J. Berrigan, N. Poulsen, N. Kröger, K. H. Sandhage
Chem. Mater. 21, 5704-5710, 2009.

Protein-enabled synthesis of monodisperse titania nanoparticles on and within polyelectrolyte matrices
E. Kharlampieva, J. M. Slocik, S. Singamaneni, N. Poulsen, N. Kröger, R. R. Naik, V. V. Tsukruk
Adv. Funct. Mat. 19: 2303-2311, 2009.

2008

Diatoms - from cell wall biogenesis to nanotechnology
N. Kröger, N. Poulsen
Annu. Rev. Genet. 42, 83-107, 2008.

The Phaeodactylum genome reveals the evolutionary history of diatom genomes
C. Bowler, et al.
Nature. 456, 239-244, 2008.

Bio-enabled Surface-mediated Growth of Uniform Titania Nanoparticles
E. Kharlampieva, T. Tsukruk, J. M. Slocik, H. Ko, N. Poulsen, R. R. Naik, N. Kröger, V. V. Tsukruk
Adv. Mater. 20, 3274-3279 (2008).

Identification of peptides capable of inducing the formation of titania but not silica via a subtractive bacteriophage display approach
Y. Fang, N. Poulsen, M. B. Dickerson, Y. Cai, S. E. Jones, R. R. Naik, N. Kröger, K. H. Sandhage
J. Mater. Chem. 18, 3871-3875, 2008.

2007

Biochemistry and Molecular Genetics of Silica Biomineralization in Diatoms
N. Kröger, N. Poulsen
Handbook of Biomineralization - Biological Aspects and Structure Formation (E. Baeuerlein, ed.), Wiley-VCH, p. 43-58, 2007.

Silica immobilization of an enzyme via genetic engineering of the diatom Thalassiosira pseudonana
N. Poulsen, C. Berne, J. Spain, N. Kröger
Angew. Chem. Int. Ed. 46, 1843-1846, 2007.

2006

Bio-enabled synthesis of Rutile (TiO₂) at ambient temperature and neutral pH
N. Kröger, M. B. Dickerson, G. Ahmad, Y. Cai, M. S. Haluska, K. H. Sandhage, N. Poulsen, V. C. Sheppard
Angew. Chem. Int. Ed. 45, 7239-7243, 2006.

Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae)
N. Poulsen, P. M. Chesley, N. Kröger
J. Phycol. 42, 1059-1065, 2006.

2005

Biosilica Nanofabrication in Diatoms: The Structures and Properties of Regulatory Silaffins
N. Kröger, N. Poulsen
MRS Proceedings, 873, K1.2 doi:10.1557/PROC-873-K1.2, 2005.

A new molecular tool for transgenic diatoms: Control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette
N. Poulsen, N. Kröger
FEBS J. 272, 3413-3423, 2005.

2004

Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana
N. Poulsen, N. Kröger
J. Biol. Chem. 279, 42993-42999, 2004.

2003

Biosilica formation in diatoms: Characterization of native silaffin-2 and its role in silica morphogenesis
N. Poulsen, M. Sumper, N. Kröger
Proc. Natl. Acad. Sci. USA 100, 12075-12080, 2003.

1999

Diatom gliding is the result of an actin-myosin motility system
N. Poulsen, I. Spector, T. P. Spurck, T. F. Schultz, R. Wetherbee
Cell Motil. Cytoskeleton 44, 23-33, 1999

1998

Phylogenetic diversity of parabasalian symbionts from termites, including the phylogenetic position of Pseudotrypanosoma and Trichonympha
P. J. Keeling, N. Poulsen, G. I. McFadden
J. Euk. Microbiol. 45, 643-50, 1998