Equipment
The Molecular Imaging and Manipulation Facility offers a range of devices and techniques, which are mainly aimed at single-molecule studies. Currently, the following techniques are available:
| Atomic Force Microscopy (AFM) | Topography imaging, Force spectroscopy and molecular manipulation |
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Spinning Disk Confocal Microscopy |
High-speed 3D confocal imaging |
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Superresolution Localization Microscopy (PALM/STORM/...) |
Superresolution fluorescence imaging |
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Total Internal Reflection Fluorescence Microscopy (TIRF) |
Structural imaging and single-molecule tracking with improved fluorescence signal-to-noise ratio and thus high localization precision |
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Fluorescence Recovery after Photobleaching (FRAP) |
Evaluation of molecular dynamics |
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Epi-Fluorescence, DIC, Darkfield and Brightfield |
Standard wide-field imaging |
If you are already a user of the MIM Facility you can make reservations in the booking calendar of the CMCB Technology Platform
Microscopes available in the Facility
Combined with a fluorescence microscope.
Equipped with petri dish heater and CellHesion module for extended z-ranges (e.g. for cells).
Operation in air and liquid environment.
Contact-, Tapping-, force modulation-, and Q.I. (force mapping imaging) mode.
Force spectroscopy/molecular manipulation.
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Dual-trap optical tweezers with 3-color confocal laser scanning for simultaneous flurescence measurement.
Molecular Manipulation.
Force Spectroscopy.
TIRF, spinning disk, epi, phase contrast;
Superresolution Localization Microscopy (STORM/PALM/...) measurements.
Equipped with two high-end EMCCD cameras, objective heater and stage heater/cooler.
Laser lines: 405, 488, 561, 647 nm.
Halogen Lamp (w/ filters for fluorescence excitation).
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Imaging in wide-field (epi, dark-field, phase contrast) and TIRF.
FRAP measurements.
Equipped with high-end EMCCD camera, objective heater and stage heater/cooler.
Laser lines: 405, 488, 561, 647 nm.
LEDs: 395, 440, 470, 510, 550, 575, 640 nm.
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Home-built iSCAT microscope;
High-speed & high-precision tracking of single molecules
Label-free detection of single molecules.
Combination with fluorescence detection is possible.