Isolation of Staphylococcus aureus in Food by Biomagnetic Separation and Novel Methods of Detection e.g. Surface Plasmon Resonance Based Biosensor

Staphylococcus aureus is one bacterial pathogen of human diseases and is also a major cause of food poisoning. Therefore, detection of this bacterial contamination in food is very important for public health protection. Traditional methods are used to detect S. aureus in food requiring a few day and may involve selective enrichment followed by isolation from selective agar and confirmed by biochemical tests. One alternative method for rapid pre-enrichment or directly concentration without enrichment is biomagnetic separation (BMS) combination with specific ligand to shorten time.To achieve the goal of this research to be rapid and accurate in detection of S. aureus cells, this project will be focused on:

• to select specific peptides to S. aureus using phage display,
• to apply biomagnetic separation to capture and enrich S. aureus in buffer and food matrix sample particular in meat products,
• to establish biosensors to rapidly detect S. aureus cells such as Surface Plasmon Resonance (SPR) and real-time polymerase chain reaction (PCR).

Project financing:

Deutscher Akademischer Austauschdienst (DAAD)

Term of project:

01.10.2006 - 31.03.2010

Contact:

Juthong, Bley