Optimization of Biomagnetic Separation with variable magnetic fields

Rapid detection of pathogens is essential for estimating health risks, and enrichment of the pathogens is generally required to reach detection limits of current analytical methods. Biomagnetic separation is ideal for such enrichment because of its high selectivity and efficiency. However, efficient mixing of magnetic particles and cells is essential to ensure high separation success. A conceptual design of an experimental device for this purpose is shown in the figure. It generates variable magnetic fields, which can accelerate magnetic particles in a highly controlled fashion, thereby increasing their velocity relative to cells in the same vial. Thus, it increases rates of collision between the particles and cells, enhancing the efficiency of biomagnetic separation.
The device has three magnetic cores (1), with the central core reversely oriented relative to the others. To generate the magnetic fields each magnetic core has an inductor (2). The sample (3) is positioned in the center of the pole shoe geometry of the magnets. The temperature is controlled by two Peltier elements and dissipaters (4) mounted on opposite sides of the device.

CAD-simulation with magnetic cores and inductors as well as attached Peltier elements for active cooling.

The device will be developed, refined and manufactured in a three-phase project:

The geometry of the magnetic cores will be refined at Dresden University of Technology, where both the magnetic fields and streaming of the magnetic particles (and fluid) will also be simulated.
The system (including a controlling unit and user interface) will be constructed and manufactured by our cooperative partner, Systemanalyse & Automatisierungsservice GmbH.
To test the setup separation experiments will be conducted at Dresden University of Technology.