Equipment of the Facility
Q-Exactive HF Orbitrap Mass Spectrometer (ThermoScientific)
The Q-Exactive MS is our state-of-art instrument for Quantitaive Proteomics applications. The MS is hyphenated to one of the Nanoflow-UPLC systems (Eksigent 425 or Dionex RLSC) for high resolution chromatographic separation and thus enables deep analysis of complex samples.
- Quantitative Proteomics
- (Quantitative) Analysis of Protein Post-translational Modifications
- Functional Proteomics (Co-IP/AP-MS)
Q-qTOF impact HD (Bruker) with Dionex3000 Nano-UPLC (Thermo Dionex) or Nanomate Triversa (Advion)
The main applications of the instrument system are
- (Quantitative) Analysis of Protein Post-translational Modifications
- Determination of Intact Protein Mass
- Biochemical Activity Assays, e.g. kinase or protease activity
LTQ-Orbitrap XL ETD (Thermo Scientific) with Dionex3000 Nano-HPLC (Thermo Dionex)
The LTQ-Orbitrap XL ETD MS provides three different fragmentation modes (CID, HCD, ETD); A combination that is especially benefical for the detailed characterization of PTMs or sequence validation of proteins.
- Analysis of Protein Post-translational Modifications
- Protein Identification and sequence determination
- Quality Control (QC) of recombinant protein
