Nikon Ti-E Superresolution/TIRF/Spinning Disk

Imaging Modes:

• wide-field (epi, dark-field, phase contrast)
• TIRF
• Spinning Disk
• 3D Superresolution Localization Microscopy (STORM/PALM/...)

Two high-end EMCCD cameras:

• Andor iXON Ultra 897 (WF, TIRF, Superresolution)
• Andor iXON Ultra 888 (SpinningDisk)

Additional sCMOS camera:

• Jenoptik ProgRes CT5

Objectives:

• 100x/1.49 SR Apochromat, Oil, DIC
• 60x/1.27 Plan Apo IR, Water, DIC
• 60x/1.2 Plan Apochromat VC, Water, DIC
• 60x/0.95 CFI PlanApo Lambda, Air, DIC
• 40x/0.95 CFI PlanApo Lambda, Air, DIC
• 20x/0.75 Plan Apochromat VC, Air, DIC

Fluorescence Excitation:

• Laser lines: 405, 488, 561, and 647 nm
• Halogen Lamp (w/ excitation filter wheel)

Additional Equipment:

• "Perfect Focus System" auto-focus
• Piezo z-stage
• Objective heater
• stage heater/cooler

© Jens Ehrig