Facility human iPSC lines
The facility generated hiPSC lines from consented healthy donors that can be used by scientists of TU Dresden as wildtype control lines or as parental lines for Genome Engineering projects:
CRTD1 hiPSC line
CRTD1
- hPSCreg name: CRTD1 - CRTDi004-A
- donor sex: male
- donor tissue: skin fibroblasts
- reprogrammed in collaboration with the laboratory of Prof. Dr. Min Ae Lee-Kirsch
- characterization: Pluripotency Flow Cytometry, 96 Gene qRT-PCR Array, 3 Germ Layer Differentiation, Karyotyping, Mycoplasma Test, STR
- culture conditions: hESC-qualified Matrigel®, mTeSR1™, ReLeSR™ clump passaging
- also available as single cell passaging (TrypLE Express, 10 µM Rock inhibitor) adapted line: CRTD1-sc
CRTD2 hiPSC line.
CRTD2 and CRTD3
- hPSCreg names: CRTD2 - CRTDi003-A, CRTD3 - CRTDi003-B
- donor sex: female
- donor tissue: CD34 positive peripheral blood cells
- reprogrammed in collaboration with the laboratory of Prof. Dr. Frank Buchholz
CRTD3 hiPSC line.
- characterization: Pluripotency Flow Cytometry, 96 Gene qRT-PCR Array, 3 Germ Layer Differentiation, Karyotyping, Mycoplasma Test, STR
- culture conditions: hESC-qualified Matrigel®, mTeSR1™, ReLeSR™ clump passaging
CRTD4 hiPSC line.
CRTD4 and CRTD5
- hPSCreg names: CRTD4 - CRTDi005-A, CRTD5 - CRTDi005-B
- donor sex: male
- donor tissue: BJ fibroblasts obtained from ATCC
- reprogrammed in collaboration with the laboratory of Dr. Jörg Mansfeld
CRTD5 hiPSC line.
- characterization: Pluripotency Flow Cytometry, 3 Germ Layer Differentiation, Karyotyping, Mycoplasma Test, STR
- culture conditions: hESC-qualified Matrigel®, mTeSR1™, ReLeSR™ clump passaging
- also available as single cell passaging (TrypLE Express, 10 µM Rock inhibitor) adapted lines: CRTD4-sc and CRTD5-sc
- CRTD5-sc was used in a publication from the laboratory of Dr. Volker Busskamp: doi: 10.1016/j.cels.2018.08.011