96-Gene qRT-PCR Analysis
Messenger RNA (mRNA) is extracted from human iPSCs, contaminating genomic DNA is digested by DNase I, the RNA is reverse transcribed to cDNA followed by quantitative PCR using a human embryonic stem cell PrimerArray®.
The expression of 96 genes including pluripotency, stem cell, differentiation and house keeping genes is compared to a control line (H9 hESCs) or to another hiPSC line.
Dot plot of 96 genes expressed in human iPSCs compared to the control hESC line H9 measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR).